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Method of producing nicotinamide by catalysis of rhodococcus

A technology of rhodococcus and nicotinamide, which is applied in the field of rhodococcus to catalyze the synthesis of nicotinamide, can solve the problem of low product concentration, achieve the effect of reducing concentration treatment, high total enzyme activity, and simple separation and purification process

Inactive Publication Date: 2015-07-08
ANHUI COSTAR BIOCHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above traditional biocatalysts have achieved certain results, the disadvantage is that the product concentration is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 A method for catalyzing the synthesis of nicotinamide by Rhodococcus comprises the following steps:

[0022] (1) Preparation of biocatalyst

[0023] Put 60% seed medium into 1L Erlenmeyer flask A, insert 5% strain after sterilization, and shake and culture at 30°C (120rpm) for 72h. Put 60% of the fermentation medium into the 1L Erlenmeyer flask B, put in 6% of the seed liquid after disinfection for fermentation, the ventilation rate is 0.5; the pressure is 0.05MPa; the stirring speed: 120rpm; Under fermentation 72h carry out centrifugation, collect the sediment after centrifugation, set aside,

[0024] Among them, each liter of seed medium contains the following components (g): glucose 15, yeast extract 2, urea 0.6, K 2 HPO 4 0.15, MgSO 4 0.03, NaCl0.15, ammonium molybdate 0.02, adjust pH =7.5 with 3% HCl,

[0025] Each liter of fermentation medium contains the following components (g): glucose 15, yeast extract 3, urea 0.6, K 2 HPO 4 0.15, MgSO 4 0.03...

Embodiment 2

[0028] In this example, the reaction conditions and parameters were kept consistent with those in Example 1, only the feeding times of the substrate and the biocatalyst were changed, and the influence of the feeding times on the catalytic reaction was investigated. For the rest, in this embodiment, 100 g of 3-cyanopyridine and 10 g of biocatalyst were first added to the reactor. When the concentration is lower than 0.5g / L, it starts to add, and the added amount is consistent with the initial amount, and it is continuously added for 5 times. The concentration of 3-cyanopyridine in the reaction solution was detected 5 hours after the fifth supplementary reaction, and the reaction could be terminated when the substrate concentration was less than 0.02 g / L. The reaction solution was centrifuged at 3000rpm for 20min, and the supernatant was frozen and crystallized at 0°C for 3h. After the crystallization of the product nicotinamide was completed, it was separated by refrigerated ce...

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Abstract

The invention belongs to the field of bio-chemical engineering and discloses a method of producing nicotinamide by catalysis of rhodococcus. In the method, by means of nitrile hydratase generated by the rhodococcus, a catalytic reaction is carried out between the nitrile-production hydratase and a substrate, 3-cyanopyridine, to produce the nicotinamide. In the reaction process, a method of adding the substrates and the catalyst in batches is employed for increasing the conversion rate. In the invention, a novel nitrile hydratase is employed so that the method is simple and convenient in operation processes and is less in environmental pollution. The nicotinamide product is easy to separate and is high in purity. The method is good in industrial application prospect.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a method for synthesizing nicotinamide catalyzed by rhodococcus. Background technique [0002] Nicotinamide (VB3), also known as Nicotinamide, has a molecular formula of C6H6N2O and a molecular weight of 122.13. It is a white needle crystal or powder with a melting point of 129-131°C. Soluble in water, soluble in alcohol and glycerin, insoluble in ether, slightly toxic. Its structural formula is as follows: [0003] Niacin and nicotinamide belong to B vitamins, collectively referred to as vitamin PP, which can be replaced in equal amounts in practical applications. Niacin in nature exists in liver, kidney, yeast and rice bran, and is a component of dehydrocoenzyme I or II in organisms , involved in the metabolism of carbohydrates, fats and proteins. They can be used in medicine to treat pellagra, etc., and can also be added to food and flour to supplement the vi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/12C12R1/01
Inventor 谷顺明胡玉兵黄永升韦琛鸿王晓亭
Owner ANHUI COSTAR BIOCHEM CO LTD
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