Bacteria BMJHZ-01 for degrading difenoconazole and screening method thereof

A technology of difenoconazole and a screening method, applied in the field of microorganisms, can solve the problems of soil pollution, aggravated soil pollution, slow degradation of difenoconazole, etc., and achieves the effects of quick effect, strong pertinence and short cycle

Inactive Publication Date: 2015-07-22
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially with the large-scale use of difenoconazole, the remaining difenoconazole in the soil gradually accumulates, causing serious soil pollution; in addition, the waste water and waste gas generated during the production of difenoconazole pesticides are also passed through Emissions and losses are accumulated into the soil, which further aggravates the pollution of the soil
However, difenoconazole degrades slowly in soil, and the half-lives are: 51.3d-125.8d, 43.6d-69.3d, 47.5-63.6d
Obviously cannot effectively solve the status quo of the gradual accumulation of difenoconazole in the soil

Method used

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  • Bacteria BMJHZ-01 for degrading difenoconazole and screening method thereof
  • Bacteria BMJHZ-01 for degrading difenoconazole and screening method thereof
  • Bacteria BMJHZ-01 for degrading difenoconazole and screening method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1) In order to find samples that can tolerate difenoconazole and contain a large number of bacteria to be screened, so as to obtain a higher screening effect; in this embodiment, the wastewater drainage channel of the difenoconazole production plant is selected As a sampling location, due to the high concentration of difenoconazole in this location, the surviving microorganisms may utilize difenoconazole to a greater extent. Therefore, the wastewater discharged from the difenoconazole production plant The way makes the samples for testing more purposeful and contributes to the smooth progress of separation and screening.

[0040] Take the mud sample from the sewage area of ​​the difenoconazole production plant, place the mud sample in the first basic salt liquid culture medium, the mass ratio of the mud sample to the first basic salt liquid culture medium is 1:20, and after the shaker culture Obtain 100ml of the first mixed bacterial solution, the temperature of the sha...

Embodiment 2

[0058] Example 2 The degradation rate of difenoconazole under the conditions of different culture temperatures and different pH values ​​of basal salt liquid medium

[0059] In order to obtain optimal culture conditions, the present invention optimizes the conditions during the shaker culture of the BMJHZ-01 single-bacteria liquid prepared in Example 1 using the basic salt liquid medium containing difenoconazole.

[0060] Set different culture temperatures for the BMJHZ-01 single-bacteria liquid obtained in Example 1, and set different gradients for the pH value of the basal salt liquid medium containing difenoconazole, and measure the difenoconazole under the corresponding conditions. The degradation rate of metroconazole, and then optimize its culture parameters, the specific steps are as follows.

[0061] Get 5 identical basal salt liquid mediums, inoculate the BMJHZ-01 single bacterium bacterium liquid prepared in Example 1, cultivate on a shaker, the inoculation amount is...

Embodiment 3

[0066] Example 3 Effect experiment of bacteria BMJHZ-01 degrading difenoconazole

[0067]This example verified the degradation effect of the bacteria BMJHZ-01 obtained in Example 1. By setting different concentrations of the basic salt liquid medium containing difenoconazole, the concentration of difenoconazole in the medium was detected. The residual amount further confirmed that the bacteria BMJHZ-01 has the effect of efficiently degrading difenoconazole.

[0068] The specific method is as follows:

[0069] The experiment is divided into two groups: test group and control group, each group has four basal salt liquid culture medium containing different concentrations of difenoconazole, the basic salt liquid culture medium used includes the following raw materials: sodium chloride 1.0g, diphosphate diphosphate Sodium hydrogen 1.5g, disodium hydrogen phosphate 0.5g, magnesium sulfate 0.2g, ferrous sulfate 0.01g, calcium chloride 0.02g, ammonium sulfate 1.0 and water 1000g, eac...

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Abstract

The invention relates to bacteria BMJHZ-01 for degrading difenoconazole and a screening method thereof. The bacteria BMJHZ-01 can be proliferated by taking difenoconazole as a carbon source. Therefore, the bacteria BMJHZ-01 can serve as microbes for degrading difenoconazole and can be utilized. The experiment proves that according to the bacteria BMJHZ-01, the difenoconazole in a basic salt liquid culture medium of which the concentration of difenoconazole is 60-180mg/L can be completely degraded within 7 days under the corresponding culture conditions. In the specific using process, lots of bacteria BMJHZ-01 subjected to expanding culture can be prepared into degradation agents which take the bacteria as active ingredients, and soil containing difenoconazole pesticide residues is remediated.

Description

technical field [0001] The invention relates to a bacterium BMJHZ-01 for degrading difenoconazole and a screening method thereof, belonging to the field of microorganisms. Background technique [0002] Difenoconazole, English common name is Difenoconazole, molecular formula is C 19 h 17 C l2 N 3 o 3 , and its molecular weight is 406.26. Pure difenoconazole is a colorless solid with a melting point of 76°C, a boiling point of 220°C / 4Pa, and a vapor pressure of 120nPa (20°C). Solubility (20°C): 3.3 mg / L in water, easily soluble in organic solvents. Stable at ≤300℃, low mobility in soil and slow degradation. [0003] Studies have shown that triazole fungicides are systemic fungicides, which are harmless to plants, but have extremely high biological toxicity to pathogenic fungi. Its bactericidal mechanism is that the nitrogen atom of the nitrogen-containing heterocyclic part in the structure of the triazole compound combines with the iron ion of cytochrome P-450, showing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/38
CPCB09C1/10C02F3/34C02F2101/38C12N1/20C12N1/205C12R2001/38
Inventor 赵建庄蔡慧敏魏朝俊贾临芳梁丹
Owner BEIJING UNIV OF AGRI
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