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Serum gamma-glutamyl transferase reagent and detection method thereof

A technology of glutamyl amino and transferase, which is applied in the measurement of color/spectral characteristics, etc., can solve the problems of poor anti-interference ability of reagents, rise of water blank, and influence of reagent application, etc., achieve good accuracy and stability, and improve performance , enhance the effect of stability and anti-interference ability

Inactive Publication Date: 2015-07-22
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1) The substrate L-γ-glutamyl-3-carboxy-p-nitroaniline naturally hydrolyzes with the prolongation of storage time, resulting in an increase in the water blank and affecting the application of the reagent;
[0006] 2) There are many components that affect the activity of GGT, and the anti-interference ability of the reagent is poor

Method used

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  • Serum gamma-glutamyl transferase reagent and detection method thereof
  • Serum gamma-glutamyl transferase reagent and detection method thereof
  • Serum gamma-glutamyl transferase reagent and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A stable and strong anti-interference ability serum γ-glutamyl aminotransferase detection reagent, including reagent R1 and reagent R2:

[0041] The components of reagent R1 are:

[0042] PIPES (piperazine-1,4-diethanesulfonic acid) buffer (pH=7.6, 25°C) ·························· 100mmol / L

[0043] Glycyl peptide ········································································································ 100mmol / L

[0044] BSA ·················································································································· 1g / L

[0045] Trehalose ··············································································································· 2g / L

[0046] APG (Alkyl Glycoside) ·································································································· 2g / L

[0047] preservative ·········································································································· 0.5g / L

[...

Embodiment 2

[0063] Interfering test

[0064] Take fresh mixed serum, divide it into 2 equal parts, and then divide each equal part into 5 equal parts, add different interfering substances, so that the concentration in the serum reaches the requirements in Table 2. Then, the reagents obtained in Example 1 were used to compare and measure the content of GGT in the serum at the same time as the common and recognized GGT reagents in the market. Relative deviation (%) = (measuring mean value of interference samples - measuring mean value of control samples) / measured mean value of control samples × 100%.

[0065]It can be seen from Table 2 that the reagent of Example 1 has no obvious interference on the test results when ascorbic acid ≤ 50 mg / dL, bilirubin ≤ 40 mg / dL, hemoglobin ≤ 200 mg / dL, and triglyceride ≤ 500 mg / dL. However, the reagents of the control group were significantly interfered in the presence of the above-mentioned concentration of interfering substances, which shows that the a...

Embodiment 3

[0069] correlation experiment

[0070] Utilize the formula of Example 1 to prepare the reagents, and conduct a control test with the γ-glutamyl aminotransferase kit of a company approved by the State Food and Drug Administration, which is common in the market, and detect 20 clinical serum samples at the same time, and the test results are shown in the table 3. And obtained the correlation curve of the two reagents (such as figure 1 As shown), the test results show that the correlation coefficient of the two kits is 0.9985, indicating that there is a great correlation between the two.

[0071] Table 3 Comparative detection results of the reagent in Example 1 and the common and recognized gamma-glutamyl aminotransferase assay kit in the market

[0072]

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Abstract

The invention relates to a biological detection reagent and in particular relates to a stable serum gamma-glutamyl transferase reagent having strong anti-jamming capacity and a detection method of the serum gamma-glutamyl transferase reagent, belonging to the technical field of the clinical in-vitro detection. Reagent R1 contains a buffer solution, glycylglycine, BSA (Bull Serum Albumin), trehalose, APG (alkyl glycoside) and preservative; reagent R2 contains a buffer solution, L-gamma-glutamyl-3-carboxyl-4-nitranilide, BSA, trehalose, APG and preservative; a PIPES (piperazine-1,4-Piperazinediethanesulfonic acid) buffer solution is adopted, and stabilizers (BSA and trehalose) are added, so that the stability of the reagent is improved greatly; and a novel surface active agent-alkyl glycoside is adopted, so that the measurement performance is remarkably improved and the stability and the anti-jamming capability of the reagent are enhanced.

Description

technical field [0001] The invention relates to a biological detection reagent, in particular to a stable and strong anti-interference ability serum gamma-glutamyl aminotransferase detection reagent and detection method, belonging to the technical field of clinical in vitro detection. Background technique [0002] GGT, that is, glutamyl transpeptidase, is present in tissues such as kidney, pancreas, liver, spleen, intestine, brain, lung, skeletal muscle and cardiac muscle. In the liver, it mainly exists in the hepatic cytoplasm and intrahepatic bile duct epithelium. GGT in normal human serum mainly comes from the liver. The normal value is 3~50U / L. This enzyme is only mildly to moderately elevated in acute hepatitis, chronic active hepatitis, and decompensated cirrhosis. However, in obstructive jaundice, this enzyme reverses into the blood due to excretion disorders, and in primary liver cancer, the synthesis of this enzyme in the liver is hyperactive, which can cause a s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 谭柏清李志明甘宜梧李静王春艳王绮
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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