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Tumor-targeting fusion protein drug carriers for optical imaging and photodynamic therapy

A fusion protein and drug technology, applied in the field of biomedicine, can solve the problem of poor targeting selectivity of water-soluble tumors

Active Publication Date: 2019-10-29
FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Likewise, many photosensitizers including CPZ also have their application limitations, such as water solubility and poor selectivity for tumor targeting

Method used

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  • Tumor-targeting fusion protein drug carriers for optical imaging and photodynamic therapy
  • Tumor-targeting fusion protein drug carriers for optical imaging and photodynamic therapy
  • Tumor-targeting fusion protein drug carriers for optical imaging and photodynamic therapy

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Embodiment

[0076] Construction, expression, purification and characterization of ATF-HSA fusion protein drug carrier:

[0077] The ATF-HSA fusion protein gene (SEQ ID NO.2) pPICZαA expression vector (purchased from Invitrogen, USA) was constructed by gene cloning. After identification by gene sequencing, the expression vector was electrotransformed into competent cells of Pichia pastoris (Pichia pastoris) X-33 (purchased from Invitrogen, USA). After a small amount of expression was identified, the fusion protein drug carrier was expressed in large quantities in the eukaryotic expression system, and methanol was added every 24 hours to a final concentration of 1%. After induction of expression for 4 days, centrifuge at 4°C, 7000rpm, 10min to remove the bacteria, and use sodium acetate buffer (AB) to adjust the pH to pH4.5. Centrifuge at 4°C, 10000rpm for 40min, and vacuum filter through a 0.45μm, 0.22μm filter membrane Finally, the treated mass-induced expression culture solution was dil...

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Abstract

The present invention relates to the field of biomedicine, more specifically to medical applications of fusion proteins, and provides a novel tumor-targeting fusion protein drug vector for optical imaging and photodynamic therapy, wherein the sequence is represented by SEQ ID NO.1. According to the fusion protein drug vector ATF-HSA of the present invention, the natural HSA ligand binding site FA1 in the vector is utilized to load CPZ to form a ATF-HSA:CPZ complex so as to make the water solubility of the CPZ be enhanced, provide targeting selectivity for tumor cells having high uPAR expression, and make the vector can be used for optical tumor imaging and photodynamic tumor therapy in vitro and in vivo.

Description

technical field [0001] The present invention relates to the field of biomedicine. More specifically, the present invention relates to biomedical applications of fusion proteins. Background technique [0002] Photodynamic therapy (PDT) is the use of light of a certain wavelength to activate the photoactive substance (photosensitizer) that gathers in the target cells, and the activated photosensitizer uses the dissolved oxygen in its surrounding tissue to generate a cytotoxic singlet state. Oxygen or free radicals, and then exert a killing effect on target cells as a treatment method. Photodynamic therapy is a selective, non-invasive treatment method, which has a good therapeutic effect on many diseases, especially tumors. In the process of photodynamic therapy, the selection and use of photosensitizer is the core issue. For example, asymmetric monocarboxy substituted zinc phthalocyanine (β- c arboxy p hthalocyanine z inc, CPZ) is an ideal strong photosensitizer, its max...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/64A61K41/00A61K49/00A61P35/00C12N15/62C12N15/81C12N1/19C12R1/84
Inventor 黄明东李睿陈卓胡萍陈锦灿周山勇袁彩陈松
Owner FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI