Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation and application of a bioactive scaffold with chemotactic function

A biologically active and functional technology, applied in the field of scaffold materials for repairing spinal cord injury, to achieve the effects of good survival and improved microenvironment

Active Publication Date: 2018-05-04
SUN YAT SEN UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, at home and abroad, a scaffold material that has biological activity and can chemoattract endogenous cells to migrate to spinal cord injury / transplantation has not been reported yet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation and application of a bioactive scaffold with chemotactic function
  • Preparation and application of a bioactive scaffold with chemotactic function
  • Preparation and application of a bioactive scaffold with chemotactic function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The main instrument used in the present invention, chemotactic active support and reagent are described in detail below by specific examples:

[0021] 1. Main instruments

[0022] Ultra-clean workbench (Suzhou Purification Electronic Equipment Factory); ordinary centrifuge (Kubota Japan); constant temperature water bath box (Beijing Medical Equipment Factory); 5% CO 2 Incubator (Queue USA); Inverted Phase Contrast Microscope (Olympus Japan); Fluorescence Microscope (Leica Germany); Scanning Electron Microscope (Philips Netherlands); Transmission Electron Microscope (Philips Netherlands); Laser Confocal Imaging System (Carl Zeiss Germany); Low Temperature Oven ( Shanghai Yuejin Medical Instrument Factory); high temperature oven (Shanghai Yuejin Medical Instrument Factory); high pressure sterilizer (Jiangyin Binjiang Medical Equipment Factory); constant cold box slicer (Shandon UK); ultrapure water instrument (Molsheim France); ELISA Detector (Bio-Rad USA); electrophores...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A novel bioactive scaffold for repairing spinal cord injury with chemotaxis of endogenous cell migration, promotion of endogenous cell survival and differentiation, and neuroprotective effects. Using natural silk protein extracted from silkworm cocoons as a medium, using freeze-drying technology and ethanol cross-linking agent to transform the crystal form of neurotrophic factor-3 (NT-3), retinoic acid (RA) and silk protein, cross-linking and fixing on the three-dimensional gelatin sponge in the bracket. The bioactive scaffold with chemotactic function provided by the present invention can release chemokines for a long time after being transplanted into the spinal cord to attract endogenous cells to migrate to the injury / transplantation area, release nutritional factors with neuroprotective effects, and improve the microenvironment , to achieve the purpose of attracting endogenous cells to self-repair spinal cord injury.

Description

Technical field [0001] The patent of the present invention relates to a scaffold material for repairing spinal cord injury, especially a bioactive scaffold that can attract endogenous cell migration and promote endogenous cell survival and differentiation. Background technique [0002] Currently, known scaffolds for repairing spinal cord injuries are composed of scaffolds and exogenous cells. It mainly achieves the effect of replacing lost neurons and treating spinal cord injury by transplanting exogenous cells into the injured area, while the scaffold is only used as a carrier for supporting cell attachment. However, in the process of clinical application, the transplantation of exogenous cells faces many difficulties such as source, ethics and immune rejection, which greatly limits the treatment of spinal cord injury with exogenous cell transplantation. Therefore, it is the key to find an effective way to circumvent the cell source problem of cell transplantation and meet...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/54
Inventor 曾园山李戈
Owner SUN YAT SEN UNIV