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Bitter apricot kernel essential oil extraction method and application as plant pathogenic fungus inhibitor

A technology of phytopathogenic fungi and extraction method, which is applied in the field of phytopathogenic fungal inhibitors and extraction of bitter almond essential oil. Low, high yield of essential oil, simple extraction process

Inactive Publication Date: 2015-08-12
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although amygdalin has important pharmacological effects and is also the precursor of benzaldehyde, one of the spices with the largest demand in the international market, the research on bitter almonds at home and abroad mainly focuses on the chemical components in bitter almonds and the properties of almond oil. The extraction technology and its physical and chemical indicators, the development of almond protein beverages and the application of rough processing technology, etc., have discarded other by-products, such as amygdalin as a toxic substance, which not only pollutes the environment, but also causes a huge waste of resources. waste
[0008] At present, the research on bitter almond essential oil is mainly concentrated in the fields of food, health care products, cosmetics and pharmaceuticals. So far, there has been no report of bitter almond essential oil as an inhibitor of plant pathogenic fungi

Method used

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  • Bitter apricot kernel essential oil extraction method and application as plant pathogenic fungus inhibitor
  • Bitter apricot kernel essential oil extraction method and application as plant pathogenic fungus inhibitor
  • Bitter apricot kernel essential oil extraction method and application as plant pathogenic fungus inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Antibacterial activity of bitter almond essential oil against wheat scab pathogen in vitro

[0041] Prepare bitter almond essential oil into medicament culture media with concentrations of 1.0, 0.625, 0.5, 0.3125, and 0.25 mg / mL respectively. After the culture medium in the petri dish is cooled and solidified, use a puncher to sterilize the prepared strains Cake, select the part where mycelium grows more densely, pick up the prepared fungus cake with an inoculation loop, with the mycelium facing down, stick it on the medium, and place three fungus cakes symmetrically according to the center in each petri dish, as a repeat. After the inoculation, the culture dish was placed in an artificial climate box for cultivation, the temperature was 28°C, the humidity was 80%, and the light was set to 0%. After cultivating for 72 hours, use a ruler to measure the diameter of the bacteria cake using the cross method, and calculate the bacteriostatic rate according to the formula. ...

Embodiment 2

[0046] In vitro antibacterial activity of bitter almond essential oil against tobacco Alternaria

[0047] Prepare bitter almond essential oil into medicament culture medium with concentrations of 1.25, 0.5, 0.312, 0.25, and 0.156 mg / mL respectively. After the culture medium in the petri dish is cooled and solidified, use a puncher to sterilize the prepared strains Cake, select the part where mycelium grows more densely, pick up the prepared fungus cake with an inoculation loop, with the mycelium facing down, stick it on the medium, and place three fungus cakes symmetrically according to the center in each petri dish, as a repeat. After the inoculation, the culture dish was placed in an artificial climate box for cultivation, the temperature was 28°C, the humidity was 80%, and the light was set to 0%. After cultivating for 72 hours, use a ruler to measure the diameter of the bacteria cake using the cross method, and calculate the bacteriostatic rate according to the formula. ...

Embodiment 3

[0052] In vitro antibacterial activity of bitter almond essential oil against apple rot pathogen

[0053] Prepare the bitter almond essential oil into medicament medium with concentrations of 1.25, 0.625, 0.5, 0.312, 0.25, and 0.156mg / mL respectively. Make the fungus cake, select the part where the mycelium grows densely, pick up the prepared fungus cake with the inoculation loop, the mycelium face down, and stick it on the medium, and place three fungi in each petri dish symmetrically according to the center. Pie, as a repeat. After the inoculation, the culture dish was placed in an artificial climate box for cultivation, the temperature was 28°C, the humidity was 80%, and the light was set to 0%. After cultivating for 72 hours, use a ruler to measure the diameter of the bacteria cake using the cross method, and calculate the bacteriostatic rate according to the formula. The results are shown in Table 3.

[0054] Table 3 Determination of antibacterial activity of bitter al...

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Abstract

The invention discloses a bitter apricot kernel essential oil extraction method. The extraction method comprises the following steps: removing grease oil from dry apricot kernels by a hot squeezing method, soaking the obtained powdery residual dregs into water with a pH value of 5 and a temperature of 40 DEG C for one hour to hydrolyze the amygdalin, and then extracting essential oil from the water solution by a water steam distillation method; wherein the distillation lasts for one hour. The invention further discloses an application of the bitter apricot kernel essential oil as a plant pathogenic fungus inhibitor. The extraction technology is simple, and the essential oil yield is high. The bitter apricot kernel has the advantages of low residue, safety, and easy degradation, and thus is a harmless plant-origin bacteriostatic agent.

Description

technical field [0001] The invention belongs to the field of botanical pesticides, and in particular relates to a method for extracting bitter almond essential oil and its use as an inhibitor of plant pathogenic fungi. Background technique [0002] The effective prevention and control of plant diseases is the guarantee for the efficient production and steady development of modern agriculture, and the wide application of pesticides is the main means of plant disease control. As a large agricultural country, the creation and application of new pesticides has extremely important strategic significance for the development of my country's agriculture and even the national economy. [0003] However, excessive and frequent use of chemical pesticides often leads to resistance of pests. In order to kill pests and increase food production, the research and development of new pesticides has never stopped. With the improvement of people's awareness of environmental protection and food ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/02A01N65/34A01P3/00
Inventor 耿会玲赵忠逯嫒琳于新池
Owner NORTHWEST A & F UNIV
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