Application of Bacillus amyloliquefaciens B-1619 in control of root-knot nematode disease

A technology of B-1619, amylolytic spores, applied in the fields of application, nematicides, chemicals for biological control, etc., can solve the problems that vegetable root-knot nematode has not been reported, and achieve good control effect, persistent occurrence and The effect of being popular and not polluting the environment

Inactive Publication Date: 2015-08-19
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
View PDF10 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Bacillus amyloliquefaciens is the dominant biological population of soil and plant micro-ecological flora, non-toxic, non-pathogenic, safe for humans and animals; because it can produce spores resistant to heat, drought, ultraviolet rays and organic solvents, it is an ideal Many strains with excellent properties have been successfu

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of Bacillus amyloliquefaciens B-1619 in control of root-knot nematode disease
  • Application of Bacillus amyloliquefaciens B-1619 in control of root-knot nematode disease

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0013] Example 1 Preparation of Bacillus amyloliquefaciens B-1619 water dispersible granules

[0014] Raw materials: Calculated by mass percentage, binder magnesium aluminum silicate 6%, dispersant NNO4%, disintegrant ammonium sulfate 10%, wetting agent sodium lauryl sulfate, Bacillus amyloliquefaciens B-1619 biocontrol bacteria 30% of the bacterial liquid, the rest is kaolin.

[0015] Preparation method: Put all the raw materials except Bacillus amyloliquefaciens B-1619 biocontrol bacteria liquid into a trough mixer (DH-200) at a rate of 24 rpm and mix uniformly for 9-10 minutes; then put the materials in Double spiral mixer (DSH-1M 3 ), add the Bacillus amyloliquefaciens B-1619 biocontrol bacteria liquid at a speed of 1460 rpm and mix for 2-3 minutes; then put the mixed material into the rotary granulator (ZLB-300) for extrusion Press granulation; the particle size of the granules is 1.0-1.5 mm. After granulation, they are put into a fluidized bed granulation coating machine...

Example Embodiment

[0018] Example 2 Application of Bacillus amyloliquefaciens B-1619 water dispersible granules in controlling tomato root knot nematodes

[0019] From August 2014 to January 2015, a demonstration test of Bacillus amyloliquefaciens biocontrol bacteria B-1619 water dispersible granules to control tomato root knot nematodes was carried out in Qianjin Village, Tangzhang Town, Tongshan District, Xuzhou City.

[0020] Experimental group: using the Bacillus amyloliquefaciens B-1619 water dispersible granules obtained in Example 1;

[0021] Control group: the drug used is abamectin;

[0022] Blank control: do not use any drugs.

[0023] 1. Experimental method:

[0024] The method of the experimental group is:

[0025] (1) Facility vegetable seedlings used for transplanting fixed seedlings must be crop seedlings that use substrate plugs to raise seedlings, to prevent planting seedlings that have been infected by root-knot nematodes;

[0026] (2) First open the planting ditch hole in the field, and ...

Example Embodiment

[0045] Example 3 Application of Bacillus amyloliquefaciens B-1619 water dispersible granules in controlling cucumber root knot nematodes

[0046] From February 2014 to July 2014, a demonstration test of Bacillus amyloliquefaciens biocontrol bacteria B-1619 water dispersible granules to control cucumber root knot nematodes was carried out in Qianjin Village, Tangzhang Town, Tongshan District, Xuzhou City. Experimental method and Example 2 Same, the experimental results are shown in Table 2:

[0047] Table 2 The effect of Bacillus amyloliquefaciens biocontrol bacteria B-1619 water dispersible granules against cucumber root knot nematodes

[0048]

[0049] The results of the greenhouse test showed that the Bacillus amyloliquefaciens biocontrol bacteria B-1619 water dispersible granules had a better control effect on cucumber root knot nematodes.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses an application of Bacillus amyloliquefaciens B-1619 in control of root-knot nematode disease. A Bacillus amyloliquefaciens B-1619 water dispersible granule with the bacterium content of at least 1.2*10<8>cfu/mL is used to control root-knot nematode disease of protected vegetables, and the water dispersible granule comprises 6% of a binder, 4% of a dispersant, 10% of a disintegrating agent, 2% of a wetting agent, 20% of a Bacillus amyloliquefaciens B-1619 biocontrol bacterium liquid, and the balance of a filler. The Bacillus amyloliquefaciens is colonized to the body surface or the internal of plant rhizosphere, and competes with root-knot nematodes on nutrition surrounding the plants to deteriorate the survival environment of the root-knot nematodes, so the Bacillus amyloliquefaciens B-1619 has the advantages of good control effect, non-toxicity, no pathogenicity, safety to human and animals, no pollution to environment, and effective and lasting control of the generation and popularization of the root-knot nematodes of the protected vegetables.

Description

technical field [0001] The invention relates to the field of biological control, in particular to the application of bacillus amyloliquefaciens B-1619 in preventing and controlling root-knot nematode diseases. Background technique [0002] Facility vegetables refer to vegetables grown in greenhouses, greenhouses, small sheds, or mulched with plastic film, and have become a pillar industry in modern agricultural production and the main way for farmers to increase their income. In recent years, with the increase of the planting area of ​​protected vegetables and successive years of planting, root-knot nematodes in protected vegetables have been increasing year by year, and have risen to become an important disease in vegetable production. Root-knot nematode (Meloidogyne) is a highly specialized omnivorous phytopathogenic nematode, which can infect more than 3000 kinds of plants and harm a variety of vegetable crops, such as cucumber, loofah, cowpea, kidney bean, eggplan...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01N63/00A01N25/14A01P5/00
Inventor 陈志谊蒋盼盼陆凡明亮刘邮洲
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap