Underwater transparent silicon dioxide nanofiber substrate as well as preparation method of substrate and application of substrate to capture of circulating tumor cells

A technology of silica and nanofibers, which is applied in the treatment of tumor/cancer cells, animal cells, fibers, etc., can solve the problems of inability to achieve transparency and meet the requirements of tumor cells, and achieve high detection efficiency and sensitivity, good biological High compatibility and production efficiency

Inactive Publication Date: 2015-08-19
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of these substrates can be transparent and cannot meet the requirements of direct monitoring of the captured tumor cells.

Method used

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  • Underwater transparent silicon dioxide nanofiber substrate as well as preparation method of substrate and application of substrate to capture of circulating tumor cells
  • Underwater transparent silicon dioxide nanofiber substrate as well as preparation method of substrate and application of substrate to capture of circulating tumor cells
  • Underwater transparent silicon dioxide nanofiber substrate as well as preparation method of substrate and application of substrate to capture of circulating tumor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1: Polyvinylpyrrolidone (PVP) (Mw≈1,300,000) and polyethylene oxide-polypropylene oxide-polyethylene oxide triblock copolymer (P123) are used as structure directing agents. Dissolve 1.5 g of TEOS, 0.5 g of P123, and 1.18 g of PVP in 10 g of ethanol, and stir magnetically at room temperature for 1 hour to obtain a homogeneous solution. A hydrolysis catalyst of TEOS, namely 0.1 g of 2M HCl solution, was added dropwise to the homogeneous solution. It was then heated at reflux at 70 °C for 0.5 h.

[0028] Step 2: If figure 1 As shown, the spinning voltage is 15kV, the No. 6 needle, the ultra-clean glass sheet is used as the substrate, the substrate is covered with aluminum foil, and the receiving distance is 20cm. The electrostatic spinning process of silica composite nanofibers is carried out. According to the thickness of the spinning fiber , the spinning time was determined to be 45min, and silica composite nanofibers were prepared on the quartz plate substrate. ...

Embodiment 2

[0038] Step 1: polyvinylpyrrolidone (PVP) (Mw≈1,300,000), polyethylene oxide-polypropylene oxide-polyethylene oxide triblock copolymer (P123) as a structure-directing agent. Dissolve 1.5g of TEOS, 0.5g of P123, and 1.18g of PVP in 10g of ethanol, and magnetically stir for 1 hour at room temperature to obtain a homogeneous solution. The TEOS hydrolysis catalyst, i.e. 0.1 g of 2M HNO 3 Was gradually added dropwise to the homogeneous solution. It was then heated at reflux at 75 °C for 0.5 h.

[0039] Step 2: Spinning voltage is 20kV, No. 6 needle, ultra-clean mica sheet is used as the substrate, aluminum foil is placed under the substrate, and the receiving distance is 30cm. The electrospinning process of silica composite fiber is carried out. According to the thickness of the spun fiber, The spinning time was 90 minutes, and a silica composite nanofiber layer with a thickness of 5 μm was obtained.

[0040] Step 3: Place the substrate covered with the silica composite nanofibe...

Embodiment 3

[0048] Step 1: polyvinylpyrrolidone (PVP) (Mw≈1,300,000), polyethylene oxide-polypropylene oxide-polyethylene oxide triblock copolymer (P123) as a structure-directing agent. Dissolve 1.5g of TEOS, 0.5g of P123, and 1.18g of PVP in 10g of ethanol, and stir magnetically for 1 hour at room temperature to obtain a homogeneous solution. The hydrolysis catalyst of TEOS, namely 0.1 g of 2M acetic acid, was gradually added dropwise into the solution. The solution was then heated at reflux at 80 °C for 3 h.

[0049] Step 2: Spinning voltage is 30kV, needle No. 6, with ultra-clean quartz sheet as the base, aluminum foil is placed underneath, and the receiving distance is 5cm, and the electrospinning process of silica composite nanofiber is carried out. According to the thickness of the spun fiber, The spinning time was 60 min, and a silica composite nanofiber layer with a thickness of 3 μm was obtained.

[0050] Step 3: Place the substrate covered with the silica composite nanofiber l...

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Abstract

The invention discloses an underwater transparent silicon dioxide nanofiber substrate as well as a preparation method of the substrate and application of the substrate to capture of circulating tumor cells. The preparation method of the substrate comprises the following steps: coating the surface of the substrate with a hydrolyzed tetraethoxy polymer solution by virtue of an electrospinning technique, then carrying out heat preservation by a drying box for further hydrolyzing; and finally burning the substrate at the high temperature to obtain the underwater transparent silicon dioxide nanofiber substrate; the substrate can be used for enriching and detecting circulating tumor cells after being further modified by a biochemistry surface antibody. The substrate has the characteristics of being high in efficiency of capturing the circulating tumor cells and high sensitivity and can be applied to clinical diagnosis of patients with cancer; meanwhile, the tumor cells captured by the substrate are high in activity; the substrate can be used for monitoring in real time and providing relevant information such as cellular morphology and has potential significance to the subsequent research of the tumor cells.

Description

technical field [0001] The invention relates to the technical fields of functional materials, biomedical materials and nanomaterials, in particular to a method for preparing an underwater transparent silica nanofiber substrate and its use for the enrichment and detection of circulating tumor cells. Background technique [0002] Circulating tumor cells, referred to as CTCs. It refers to the release of tumor cells from the primary tumor or metastases into the peripheral blood circulation due to spontaneous or diagnostic and therapeutic operations, forming circulating tumor cells. As a result, more tumors grow in organs farther away from the lesion, which is the main reason for the vast majority of malignant tumor patients to have distant metastasis and recurrence after surgery. In 1869, Thomas Ashworth first observed CTCs in a patient with metastatic cancer, and they speculated that the recognition of cells from circulating tumor cells found in the blood was likely responsibl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F9/08D01F1/10D01D5/00D06M13/513D06M13/418D06M10/02C12N5/09
Inventor 马兰王女赵勇
Owner BEIHANG UNIV
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