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Method for treating pesticide residues and heavy metal mercury in sewage

A technology for pesticide residues and heavy metals, applied in chemical instruments and methods, biochemical equipment and methods, filtration treatment, etc., can solve problems such as secondary pollution of soil and water sources, achieve improved treatment effects, good application prospects, and reduce reaction time Effect

Inactive Publication Date: 2015-09-02
刘昱宁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large amount of poisonous gas is released during the process, and a large amount of heat energy is required
There are also problems with burial, mainly in the secondary pollution of the surrounding soil and water sources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The treatment of embodiment 1 mercury and pesticide residual sewage

[0026] When the sewage enters the water, the concentration of mercury is 30mg / L, dimethoate 20mg / L, fenitrothion 20mg / L, methomyl 20mg / L.

[0027] Pretreatment: remove the suspended solids in the treated sewage by filtration and sedimentation;

[0028] Main treatment: add the bacterium Pseudomonas putida SPl CGMCC No. 3887, which has been cultured to the logarithmic growth phase, disclosed in CN 102373161A, into the sewage, and flow culture for 24 hours under normal temperature conditions, and the sewage can be removed HG;

[0029] Post-treatment: the sewage is treated overnight through the zeolite and activated carbon adsorption pool again to adsorb pesticide residues;

[0030] After the treated sewage is disinfected, it is directly discharged or reused. After testing, the concentration of mercury was 2.2mg / L, dimethoate 15.4mg / L, fenitrothion 14.3mg / L, and methomyl 16.4mg / L.

Embodiment 2

[0031] Example 2 Transformation and acquisition of degradable pesticide genes

[0032] According to the method disclosed in CN1970741A, CYP9G2 and POR genes were cloned. The POR gene was modified as follows, and the corresponding Asp44Ser (indicating that the 44th Asp was mutated to Ser), Gln74Tyr, Leu96Lys, Asp137Ala, His199Gly, Ile231Gly, Asp296Glu, Gly316Asn, Asp346Glu, Pro381Cys, Tyr484Lys, Ser519Arg, Gisl were transformed by multiplex PCR , the mutation sites were respectively introduced into the protein shown in GenBank: L19897, so as to construct different mutant POR genes (can be obtained by referring to the preparation method of the prior art). The mutated POR gene and CYP9G2 gene were respectively connected to the expression vector according to the method for preparing CN1970741A, and the successfully verified recombinant plasmid was transformed into the Pseudomonas putida SP1 CGMCC No. 3887 strain, which was successfully constructed by PCR detection.

Embodiment 3

[0033] Example 3 Verification of sewage degradation effect

[0034] The strains containing different mutant proteins were respectively followed the method of Example 1 to verify the treatment effect on sewage. When the sewage enters the water, the concentration of mercury is 30mg / L, dimethoate 20mg / L, fenitrothion 20mg / L, methomyl 20mg / L. The strains and bacteria transformed with unmutated CYP9G2 and POR genes were used as controls. The specific results are as follows:

[0035] strain Mercury concentration (mg / L) Dimethoate (mg / L) Fenitrothion (mg / L) Methomyl (mg / L) Asp44Ser strain (SR1 strain) 0.2 0.6 0.7 0.8 Gln74Tyr strain (SR2 strain) 0.1 0.5 0.6 0.6 Leu96Lys strain (SR3 strain) 0.2 0.5 0.4 0.5 Asp137Ala strain (SR4 strain) 0.2 0.4 0.4 0.7 His199Gly strain (SR5 strain) 0.1 0.5 0.5 0.4 Ile231Gly strain (SR6 strain) 0.2 0.6 0.6 0.6 Asp296Glu strain (SR7 strain) 0.3 0.3 0.4 0.5 Gly316Asn str...

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PUM

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Abstract

The invention relates to the field of sewage treatment, in particular to a method for reducing mercury and pesticide residues in sewage. Mercury pollution in sewage can be effectively eliminated through the method, and the advantages of being high in efficiency, low in nutritional requirement, easy to operate, high in safety and the like are achieved; compared with existing other technologies, the pollution abatement cost of mercury and pesticide residues in sewage is lowered obviously, the pollution abatement efficiency of mercury and pesticide in sewage is improved, and the practical application value is great.

Description

technical field [0001] The invention belongs to the field of sewage treatment, in particular to the treatment of pesticide residues and heavy metal mercury in sewage, and belongs to the technical field of comprehensive sewage treatment. Background technique [0002] In recent decades, chemical pesticides have always been the main means of controlling pests, weeds and rodents in garden agriculture, and their sales have increased year by year. While controlling diseases, pests, weeds and rodents and increasing production, chemical pesticides also destroy the ecology and pollute the environment. 70% of the annual amount of more than 500,000 tons of pesticides will flow into the soil of rivers and rivers. Moreover, some pesticides degrade slowly, and pesticides in the air The concentration will be maintained for a long time, and the pollution of water and food directly endangers human health and animal safety, and the polluted surface water directly pollutes groundwater. As the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F9/04C02F9/14C02F101/20
CPCC02F1/00C02F1/001C02F1/281C02F1/283C02F1/50C02F1/52C02F1/5245C02F1/56C02F3/34C02F3/342C02F9/00C02F2001/007C02F2101/20C02F2101/306C02F2301/08C02F2303/04C12N9/0042C12Y106/02004
Inventor 朱小明
Owner 刘昱宁
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