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Helicobacter pylori antigen colloidal gold detection kit

A technology of Helicobacter pylori and a detection kit, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as complex treatment plans, recurrence, and further confirmation of the relationship, and achieve rapid detection, quick and easy operation, and accurate results Effect

Active Publication Date: 2015-09-09
SHANGHAI CHEMTRON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

HP can also be detected in 40-70% of non-ulcer dyspepsia patients, but the relationship between the two remains to be further confirmed
On the other hand, the treatment plan for HP is complicated and the short-term effect is good, but there is still the possibility of recurrence

Method used

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  • Helicobacter pylori antigen colloidal gold detection kit
  • Helicobacter pylori antigen colloidal gold detection kit
  • Helicobacter pylori antigen colloidal gold detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The preparation of embodiment 1 test paper card of the present invention

[0043] 1) Use the pretreatment buffer to pretreat the gold standard pad. The pretreatment buffer is: 18-crown-66mg / ml sodium chloride 0.3mg / ml, glycine 2.0mg / ml aqueous solution, pH=7.4, pretreatment The specific steps of the treatment are: soak the gold standard pad in the pretreatment solution for 2 hours, take it out and dry it at 37°C; The gold-labeled pad, the gold-labeled pad coated with Anti-Helicobacter pylori antibody [51-13] (ab8081), the mass ratio of colloidal gold and monoclonal antibody in the solution is 5:1, and the concentration of the solution is 10mg / ml , the spray volume is 4ul / cm;

[0044] 2) Spray 1 mg / ml Anti-Helicobacter pylori antibody [SPM526], prediluted (ab82084) solution and goat anti-mouse IgG polyclonal antibody (purchased from MAXMED LABORATORIES INC. ) solution, spraying amount is 1ul / cm, makes the nitrocellulose film after coating;

[0045] 3) Paste the sample...

Embodiment 2

[0046] The preparation of embodiment 2 comparison kit

Embodiment 3

[0051] The sensitivity experiment of embodiment 3 detection kits

[0052] Get the Helicobacter pylori antigen colloidal gold detection kit that embodiment 1, 2 make, kit is placed on the horizontal surface, detects respectively the Helicobacter pylori antigen standard, each concentration was repeated 5 times. Take 2-3 drops of the sample solution with a straw and add it to the sample tank, and read the result 8-15 minutes after adding the sample.

[0053] Table 1 Kit sensitivity results

[0054]

[0055]

[0056] The results in Table 1 confirm that the Helicobacter pylori antigen colloidal gold detection kit prepared by the present invention has a sensitivity ≤ 100 pg / ml, and has very high sensitivity. A sensitivity test was further performed on the detection kit of Example 2, and it was found that the sensitivity of the detection kit of Example 2 was ≥ 1 ng / ml.

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Abstract

The invention relates to the field of biological detection, in particular to a helicobacter pylori antigen colloidal gold detection kit, and a preparation method and an application thereof. The helicobacter pylori antigen colloidal gold detection kit provided by the invention comprises a test paper card, wherein the test paper card comprises a bottom plate, a sample pad, a gold mark pad, a nitrocellulose membrane and an absorbent pad; the sample pad, the gold mark pad, the nitrocellulose membrane and the absorbent pad are located on the surface of the bottom plate and are sequentially arranged from a sampling end; the gold mark pad comprises a colloidal gold-marked anti-helicobacter pylori monoclonal antibody-1; and the nitrocellulose membrane is coated with a detection line and a quality control line. The helicobacter pylori antigen colloidal gold detection kit provided by the invention has sensitivity and specificity and has the advantages of being fast, simple and convenient to operate, accurate in result, economical and practical.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a Helicobacter pylori antigen colloidal gold detection kit and its preparation method and application. Background technique [0002] Helicobacter pylori (HP for short) is a multi-flagellated, spiral-shaped Gram-negative bacterium that lives in the human gastric mucosa. In 1983, Australian scientists Marshall and Warren first successfully isolated Helicobacter pylori from gastric mucosal biopsy specimens of patients with chronic active gastritis, and won the 2005 Nobel Prize in Physiology and Medicine for their contributions to the field of Helicobacter pylori research. Helicobacter pylori is obligate microaerophilic, requires high nutrition for growth, and grows slowly. It usually takes 3 to 5 days to form colonies. It has a stronger tolerance to low pH than ordinary bacteria. HP has a special affinity for gastric epithelial cells. Under natural conditions, a large amount of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/531G01N33/577
CPCG01N33/531G01N33/558G01N33/56911G01N33/577G01N2333/195
Inventor 姜伟民
Owner SHANGHAI CHEMTRON BIOTECH
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