A method for synthesizing salidroside by attapulgite-loaded black Brin seed β-glucosidase cross-linked polymer
A technology of glucosidase and cross-linked polymer, which is applied in the field of synthesizing salidroside by attapulgite-loaded β-glucosidase cross-linked polymer of blackberry seeds, which can solve the problem of low concentration and high cost of salidroside , Low recovery rate of enzyme activity and other problems, to achieve the effect of improving the utilization rate of enzyme, low price, and improving the recovery rate of enzyme activity
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Embodiment 1
[0032] Embodiment 1: extracting black pulin seed β-glucosidase enzyme liquid 1
[0033] 1) Dehull and crush the black pudding seeds to obtain black pudding seed powder with a particle size of 100 mesh, then add 50g / L acetic acid-sodium acetate buffer solution with pH5.4, and then add Zhan black pudding seed powder The pectinase with a mass fraction of 1% was mixed evenly, incubated at a constant temperature of 40°C with shaking for 1 hour, the obtained solution was centrifuged, the supernatant was vacuum freeze-dried, and the powder was ground through a 60-mesh sieve to obtain a dry powder;
[0034]2) Step 1) Add 25 times the dry powder volume of phosphate buffer solution (concentration: 50 mM, pH: 7.4) as the extract, stir at 30 r / min for 1 h at 4°C, centrifuge, collect the extract, Add ammonium sulfate powder with 40% of the mass of the extract, stir for 30 min, centrifuge again, take the supernatant, add ammonium sulfate powder with a mass of 65% of the supernatant, stir at...
Embodiment 2
[0036] Embodiment 2: extracting black pulin seed β-glucosidase enzyme liquid 2
[0037] 1) Dehull and crush the black pudding seeds to obtain black pudding seed powder with a particle size of 100 mesh, then add 50g / L acetic acid-sodium acetate buffer solution with pH5.4, and then add Zhan black pudding seed powder The pectinase with a mass fraction of 2% was mixed evenly, incubated at a constant temperature of 40°C for 2 hours with shaking, the obtained solution was centrifuged, the supernatant was vacuum freeze-dried, and the powder was ground through a 60-mesh sieve to obtain a dry powder;
[0038] 2) Step 1) Add phosphate buffer solution (concentration: 50 mM, pH: 7.4) 50 times the volume of the dry powder to the dry powder as the extract, stir at 30 r / min for 1 h at 4°C, centrifuge, and collect the extract. Add ammonium sulfate powder with a mass of 40% of the extract, stir at 30r / min for 30 min, centrifuge again, take the supernatant, add ammonium sulfate powder with a ma...
Embodiment 3
[0040] Embodiment 3: extracting Heblin seed β-glucosidase enzyme liquid 3
[0041] 1) Dehull and crush the black pudding seeds to obtain black pudding seed powder with a particle size of 100 mesh, then add 50g / L acetic acid-sodium acetate buffer solution with pH5.4, and then add Zhan black pudding seed powder The pectinase with a mass fraction of 1.5% was mixed evenly, incubated at 40°C for 1.5 hours with constant temperature and shaking, the obtained solution was centrifuged, the supernatant was vacuum freeze-dried, and the powder was ground through a 60-mesh sieve to obtain a dry powder;
[0042] 2) Step 1) Add 35 times the dry powder volume of phosphate buffer solution (concentration: 50 mM, pH: 7.4) as the extract, stir at 30 r / min for 1 h at 4°C, centrifuge, collect the extract, Add ammonium sulfate powder with a mass of 40% of the extract, stir at 30r / min for 30 min, centrifuge again, take the supernatant, add ammonium sulfate powder with a mass of 65% of the supernatant...
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