Lactic dehydrogenase isozyme 1 detection reagent and method

Abstract

The invention relates to a detection reagent for detecting lactic dehydrogenase isozyme 1 through a chemical inhibition method. The detection reagent comprises a reagent R1 and a reagent R2. The detection reagent is characterized in that the reagent R1 comprises a biological buffer solution in which a solute in the reagent R1 ranges from 50 mmol / L to 150 mmol / L in concentration, 1.0 mmol / L to 1.5 mmol / L of L-lithium lactate, 1.5 mmol / L to 2.5 mmol / L of sodium perchlorate, 1ml / L to 5ml / L of hydroxy ethidene diphosphonic acid, 0.1 g / L to 1g / L of octadecyl trimethyl ammonium bromide, 0.1 ml / L to 1 ml / L of polyoxyethylene alkyl ether, and 0.5 ml / L to 5 ml / L of preservative. The reagent R2 comprises a buffer solution in which a solute in the reagent R2 ranges from 50 mmol / L to 150 mmol / L in concentration, 8-12 mmol / L NAD+ and 0.5-5 ml / L of preservative. The lactic dehydrogenase isozyme 1 detection reagent has the advantages of being high in accuracy and cheap in price.

Description

technical field [0001] The invention relates to the detection field of lactate dehydrogenase isoenzyme 1, in particular to a detection reagent and a detection method for detecting lactate dehydrogenase isoenzyme 1 by using a chemical inhibition method. Background technique [0002] Lactate deoxygenase has five isozyme forms, namely LDH1, LDH2, LDH3, LDH4, and LDH5, which can be separated by electrophoresis. LDH1 and LDH2 are the most abundant in human myocardium, kidney, and red blood cells. Liver and striated muscle are dominated by LDH4 and LDH5. There are more LDH3 in the spleen, pancreas, thyroid, and adrenal glands. Lactate dehydrogenase isoenzyme is one of the indicators to observe myocardial disease, liver and gallbladder disease, etc. [0003] Lactate dehydrogenase isozymes refer to a group of enzymes that catalyze the same reaction (lactate-pyruvate) but have different structures. It is widely distributed in various tissues of the body, and most of LDH1 is distr...

AI Technical Summary

Problems solved by technology

The detection methods for lactate dehydrogenase isoenzyme mainly include chemical continuous detection method, and the chemical inhibitors that can be used mainly include perchlorate, hydroxyl compound and guanidine thiocyanate. These methods have advantages and disadvantages, among which The electrophoresis method is accurate and reliable. It can understand the situation of the entire LDH isoenzyme spectrum. The information is comprehensive. The disadvantages are poor sensitivity, long operation time, and high requirements for instruments and operators. The results of ion exchange chromatography are also very good. Accurate, but the operation requirements are also very high and the operation time is also very long, while the chemical inhibition method and the immune method are easy to operate and the use time is short, which is very suitable for promotion. Compared with the immune method, the price of the chemical inhibition method is lower. However, due to the fact that there are many inhibitors that can be selected in the chemical inhibition method, the specificity of the detection reagent will be poor due to the different types of inhibitors and the different contents of the inhibitors, and it is easy to be interfered by other lactate dehydrogenase isoenzymes, or excessive Inhibition situation, the present invention according to this problem, establishes a kind of detection reagent of serum lactate dehydrogenase isoenzyme 1 that a kind of highly specific chemical inhibition method is detected on the basis of chemical inhibitor method

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