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Soybean-source anti-oxidative peptide nano-liposome and preparation method thereof

A soybean antioxidant peptide and nanoliposome technology, which is applied in the directions of liposome delivery, peptide/protein components, antitoxins, etc., can solve the problems of increased viscosity, microbial growth, complicated operation, etc., and achieves low cost of raw materials, The effect of method innovation and simple process route

Active Publication Date: 2015-09-30
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Peptides will absorb moisture when placed at room temperature, resulting in increased viscosity, and prolonged storage will also lead to the growth of microorganisms. These processes have seriously hindered the application of soybean antioxidant peptides in biology, food, chemistry, medicine, etc.
At present, there are many protection methods, such as adding protective agents and exploring synergistic effects with other functional factors, but these methods are complicated to operate, so simple and fast products that can make soybean antioxidant peptides play their functional roles need to be developed urgently

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for preparing soybean-derived antioxidant peptide nanoliposomes, comprising the steps of:

[0022] Using soybean antioxidant peptides of 3-10 kDa as raw materials, accurately weigh 15 mg of soybean antioxidant peptides, and dissolve them in PBS buffer solution with a pH value of 6 and a concentration of 0.05M at room temperature to obtain an aqueous phase system. Then weigh phospholipids and cholesterol with a mass ratio of 2:1, put them in a round bottom flask, dissolve them in ether at room temperature, and plug cotton on the bottle mouth to obtain a lipid phase system, and place them in -10 ℃ environment for standby. In a fume hood, place a medical needle on a 2mL syringe, quickly draw the aqueous phase and inject it into the lipid phase, and the lipid phase:water phase volume ratio is 5:1. The mixed phase was treated with an ultrasonic cell crushing instrument with a power of 700W and ultrasonication at -10°C for 6min to form a stable W / O emulsion. Transf...

Embodiment 2

[0028]Using 3-10kDa soybean antioxidant peptides as raw materials, accurately weigh 20mg of soybean antioxidant peptides, and dissolve them in PBS buffer solution with a pH value of 6.8 and a concentration of 0.1M at room temperature to obtain an aqueous phase system. Then weigh phospholipids and cholesterol with a mass ratio of 3:1, put them in a round-bottomed flask, dissolve them in ether at room temperature, and plug cotton on the bottle mouth to obtain a lipid phase system, and place them in -8 ℃ environment for standby. In a fume hood, place a medical needle on a 2mL syringe, quickly draw up the aqueous phase and inject it into the lipid phase, and the lipid phase:water phase volume ratio is 1:1. The mixed phase was treated with an ultrasonic cell crushing instrument with a power of 550W and ultrasonication at -8°C for 7 minutes to form a stable W / O emulsion. Transfer the mixed phase to a 100mL spin-dried bottle, and rotate it at 40°C for 12 minutes under low pressure t...

Embodiment 3

[0031] Using 3-10kDa soybean antioxidant peptides as raw materials, accurately weigh 25mg soybean antioxidant peptides, dissolve them in a PBS buffer solution with a pH value of 7.4 and a concentration of 0.5M at room temperature to obtain an aqueous phase system. Then weigh phospholipid and cholesterol with a mass ratio of 5:1, put them in a round-bottomed flask, dissolve them in ether at room temperature, and plug cotton on the bottle mouth to obtain a lipid phase system, and place them in -4 ℃ environment for standby. In a fume hood, place a medical needle on a 2mL syringe, quickly draw up the volume of the aqueous phase and inject it into the volume of the lipid phase, and the volume ratio of lipid phase:water phase is 2:1. The mixed phase was treated with an ultrasonic cell crushing instrument with a power of 500W and ultrasonication at -6°C for 9 minutes to form a stable W / O emulsion. Transfer the mixed phase to a 100mL spin-dried bottle, and evaporate under low pressur...

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Abstract

The invention belongs to the technical field of agricultural product processing, in particular to a soybean-source anti-oxidative peptide nano-liposome and a preparation method thereof. The preparation method comprises the steps of mixing, ultrasonic processing, low-pressure evaporation, vacuum evaporation, re-ultrasonic processing, adding of a freeze-drying protective additive, and freeze drying under the conditions that 3-10 kDa soybean anti-oxidative peptide is taken as a raw material, and the encapsulation efficiency, grain size and electric potential are taken as quality test indicators. The preparation method integrates a reverse evaporating method, an ultrasonic method and a freeze-drying method, and has the advantage that liposome, of which the encapsulation efficiency is 45.43% or higher, the grain size reaches 531.2 mm or lower, and the electric potential reaches -19.8 mV or lower, is obtained finally. The soybean-source anti-oxidative peptide nano-liposome and the preparation method have the advantages that the raw material cost is lower; the designed process route is simple and convenient; the method is creative; a novel way is provided for comprehensive utilization of soybean-source anti-oxidative peptide.

Description

technical field [0001] The invention belongs to the technical field of agricultural product processing, and in particular relates to a soybean-derived antioxidant peptide nanoliposome and a preparation method thereof. Background technique [0002] Soybean peptide has many physiological functions, among which the antioxidant function is remarkable. The physiological functions of soybean peptides include lowering blood pressure, lowering blood fat, lowering cholesterol, promoting energy metabolism, recovering from fatigue, strengthening muscles, anti-allergy, immunity, anti-oxidation and so on. Excessive free radicals in the body can lead to various diseases, such as emphysema, eye diseases, joint diseases, heart diseases such as atherosclerosis and diabetes, etc., causing serious damage to the body. There are many active substances in soybean protein hydrolyzate, including active peptides and activated histidine, tyrosine, etc. These active substances can improve the digest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K38/02A61P39/06
Inventor 林松毅杨睿雯段浏嘉黄延军
Owner JILIN UNIV
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