Puerarin extraction process

An extraction process and puerarin technology, applied in the field of puerarin extraction process, can solve the problems of unsatisfactory effect, limited extraction efficiency and high production cost, achieve significant economic and social benefits, facilitate transportation and storage, and meet the requirements of equipment. high effect

Inactive Publication Date: 2015-09-30
SICHUAN LYUCHAO NEW AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction of puerarin is also more and more paid attention to by people. Traditionally, the utilization of puerarin is generally directly edible or medicinal, and its beneficial components such as puerarin cannot be effectively utilized, and it is inconvenient to eat, and the taste is single; The extraction methods are generally water extraction and alcohol precipitation method, n-butanol-water extraction method, alumina decolorization method, polyacrylamide chromatography, glacial a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Take kudzu root and crush it through a 30-mesh sieve at a low temperature of -4°C, add distilled water, the weight ratio of kudzu root to distilled water is 1:7, soak it at room temperature for 5 hours, and then stir it with a mixer to obtain a kudzu root slurry;

[0021] 2. Add enzyme preparation to the slurry in step 1 to carry out enzymatic hydrolysis reaction, and perform low-intensity ultrasonic treatment at the same time;

[0022] ①When the pH value is 4.0-5.0, add pectinase 100u / g kudzu root slurry;

[0023] ②When the pH value is 5.0-6.5, add cellulase 75u / g kudzu root slurry;

[0024] ③When the pH value is 6.5-8.0, add protease 50u / g kudzu root slurry; after enzymolysis, heat up to 100°C, keep it warm for 5 minutes to inactivate the enzyme, and cool down to room temperature; Add enzyme preparation for enzymolysis, the time is 45min, the pH is 7.0, and the ultrasonic frequency is 12kHz;

[0025] 3. Carry out solid-liquid separation, remove the kudzu root slu...

Embodiment 2

[0028] 1. Take kudzu root and pulverize it at a low temperature of 0°C to pass through a 50-mesh sieve, add distilled water, the weight ratio of kudzu root to distilled water is 1:7, soak it at room temperature for 5 hours and stir it with a mixer to obtain a kudzu root slurry;

[0029] 2. Add enzyme preparation to the slurry in step 1 to carry out enzymatic hydrolysis reaction, and perform low-intensity ultrasonic treatment at the same time;

[0030] ①When the pH value is 4.0-5.0, add pectinase 100u / g kudzu root slurry;

[0031] ②When the pH value is 5.0-6.5, add cellulase 75u / g kudzu root slurry;

[0032] ③When the pH value is 6.5-8.0, add protease 50u / g kudzu root slurry; after enzymolysis, heat up to 100°C, keep it warm for 10 minutes to inactivate the enzyme, and cool down to room temperature; Add enzyme preparation for enzymolysis, the time is 50min, the pH is 7.0, and the ultrasonic frequency is 12kHz;

[0033] 3. Carry out solid-liquid separation, remove the kudzu ro...

Embodiment 3

[0036] 1. Take kudzu root and crush it through a 100-mesh sieve at a low temperature of -2°C, add distilled water, the weight ratio of kudzu root to distilled water is 1:3, soak it at room temperature for 8 hours and stir it with a mixer to obtain a kudzu root slurry;

[0037] 2. Add enzyme preparation to the slurry in step 1 to carry out enzymatic hydrolysis reaction, and perform low-intensity ultrasonic treatment at the same time;

[0038] ①When the pH value is 4.0-5.0, add pectinase 100u / g kudzu root slurry;

[0039] ②When the pH value is 5.0-6.5, add cellulase 75u / g kudzu root slurry;

[0040] ③ When the pH value is 6.5-8.0, add protease 50u / g kudzu root slurry; after enzymolysis, heat up to 100°C, keep it warm for 15min to inactivate the enzyme, and cool down to room temperature; Add enzyme preparation for enzymolysis, the time is 55min, the pH is 8.0, and the ultrasonic frequency is 12kHz;

[0041] 3. Carry out solid-liquid separation, remove the kudzu root slurry resi...

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PUM

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Abstract

The invention discloses a puerarin extraction process, relating to extraction of effective components in Chinese herbal medicine plants and belonging to the field of extraction processes. The puerarin extraction process is characterized by comprising the following steps: taking kudzu root, pulverizing at the low temperature of -4-0 DEG C, passing through a 30-100-mesh screen, adding distilled water which is 1-7 times by weight of kudzu root, immersing at room temperature for 5-8 hours, stirring with a stirrer to obtain a kudzu root slurry, adding an enzyme preparation to perform enzymolysis reaction while performing low-intensity ultrasonic treatment, carrying out solid-liquid separation and collecting the filtrate to obtain the kudzu root extract concentrated solution. The technique for extracting effective components of kudzu root has the advantages of high production efficiency, low requirements for equipment, high controllability, high operability and low energy consumption, is suitable for large-scale production of the kudzu root product, is beneficial to overall development and utilization of kudzu root resources, and has obvious economic benefit and social benefit. The kudzu root beverage prepared by the technique has the advantages of high nutrient content and good and diversified tastes, is convenient to drink, and is convenient for transportation and storage.

Description

technical field [0001] The invention relates to an extraction of effective components of Chinese herbal medicine plants, which belongs to the field of extraction technology, in particular to a puerarin extraction technology. Background technique [0002] Pueraria Lobata (Willd.) Ohwi is the dried root of Pueraria Lobata (Willd.) Ohwi, a leguminous plant. It is a commonly used natural plant for both medicine and food. It tastes sweet and cool, and is often used for soup; it contains about 12 % flavonoids, including more than 10 kinds of daidzein, daidzein, puerarin, etc., and also contain carrotin, amino acids, coumarins, etc., which have the functions of expanding blood vessels, improving blood circulation, lowering fat and blood sugar, anti-oxidation and Various functions such as scavenging free radicals. [0003] Puerarin flavonoids and puerarin extracted from pueraria root have been widely used in health food, biopharmaceutical and other fields in the United States. In ...

Claims

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Application Information

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IPC IPC(8): C07D407/04
Inventor 洪继元魏俊良
Owner SICHUAN LYUCHAO NEW AGRI SCI & TECH
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