Puerarin extraction process
An extraction process and puerarin technology, applied in the field of puerarin extraction process, can solve the problems of unsatisfactory effect, limited extraction efficiency and high production cost, achieve significant economic and social benefits, facilitate transportation and storage, and meet the requirements of equipment. high effect
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Embodiment 1
[0020] 1. Take kudzu root and crush it through a 30-mesh sieve at a low temperature of -4°C, add distilled water, the weight ratio of kudzu root to distilled water is 1:7, soak it at room temperature for 5 hours, and then stir it with a mixer to obtain a kudzu root slurry;
[0021] 2. Add enzyme preparation to the slurry in step 1 to carry out enzymatic hydrolysis reaction, and perform low-intensity ultrasonic treatment at the same time;
[0022] ①When the pH value is 4.0-5.0, add pectinase 100u / g kudzu root slurry;
[0023] ②When the pH value is 5.0-6.5, add cellulase 75u / g kudzu root slurry;
[0024] ③When the pH value is 6.5-8.0, add protease 50u / g kudzu root slurry; after enzymolysis, heat up to 100°C, keep it warm for 5 minutes to inactivate the enzyme, and cool down to room temperature; Add enzyme preparation for enzymolysis, the time is 45min, the pH is 7.0, and the ultrasonic frequency is 12kHz;
[0025] 3. Carry out solid-liquid separation, remove the kudzu root slu...
Embodiment 2
[0028] 1. Take kudzu root and pulverize it at a low temperature of 0°C to pass through a 50-mesh sieve, add distilled water, the weight ratio of kudzu root to distilled water is 1:7, soak it at room temperature for 5 hours and stir it with a mixer to obtain a kudzu root slurry;
[0029] 2. Add enzyme preparation to the slurry in step 1 to carry out enzymatic hydrolysis reaction, and perform low-intensity ultrasonic treatment at the same time;
[0030] ①When the pH value is 4.0-5.0, add pectinase 100u / g kudzu root slurry;
[0031] ②When the pH value is 5.0-6.5, add cellulase 75u / g kudzu root slurry;
[0032] ③When the pH value is 6.5-8.0, add protease 50u / g kudzu root slurry; after enzymolysis, heat up to 100°C, keep it warm for 10 minutes to inactivate the enzyme, and cool down to room temperature; Add enzyme preparation for enzymolysis, the time is 50min, the pH is 7.0, and the ultrasonic frequency is 12kHz;
[0033] 3. Carry out solid-liquid separation, remove the kudzu ro...
Embodiment 3
[0036] 1. Take kudzu root and crush it through a 100-mesh sieve at a low temperature of -2°C, add distilled water, the weight ratio of kudzu root to distilled water is 1:3, soak it at room temperature for 8 hours and stir it with a mixer to obtain a kudzu root slurry;
[0037] 2. Add enzyme preparation to the slurry in step 1 to carry out enzymatic hydrolysis reaction, and perform low-intensity ultrasonic treatment at the same time;
[0038] ①When the pH value is 4.0-5.0, add pectinase 100u / g kudzu root slurry;
[0039] ②When the pH value is 5.0-6.5, add cellulase 75u / g kudzu root slurry;
[0040] ③ When the pH value is 6.5-8.0, add protease 50u / g kudzu root slurry; after enzymolysis, heat up to 100°C, keep it warm for 15min to inactivate the enzyme, and cool down to room temperature; Add enzyme preparation for enzymolysis, the time is 55min, the pH is 8.0, and the ultrasonic frequency is 12kHz;
[0041] 3. Carry out solid-liquid separation, remove the kudzu root slurry resi...
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