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Microwave-assisted enzymatic extraction process of tea polyphenol

A microwave-assisted, enzymatic extraction technology, applied in the chemical industry, sustainable manufacturing/processing, climate sustainability, etc., can solve the problems of large use of organic solvents, solvent residues, etc., to improve purity, promote dissolution, and improve efficiency high effect

Inactive Publication Date: 2015-10-14
HUNAN FEIXIANG JINYANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention utilizes a solvent to extract tea polyphenols, and the product has problems such as a large amount of organic solvent used and solvent residues.

Method used

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  • Microwave-assisted enzymatic extraction process of tea polyphenol
  • Microwave-assisted enzymatic extraction process of tea polyphenol
  • Microwave-assisted enzymatic extraction process of tea polyphenol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] a) Impurity removal and cleaning: select fresh green tea leaves, remove impurities such as wood chips and branches, wash the dust on the surface of the tea leaves with clean water, drain the surface water, and set aside;

[0028] b) Pulverization of tea leaves: take the tea leaves in step a) and pulverize them to 30 mesh sieves with a pulverizer for subsequent use;

[0029] c) Adjust liquid and add enzyme: add pure water according to the material-to-liquid ratio of 1:13, adjust the tea pH to 4.0 after mixing evenly, stir evenly, add compound enzyme liquid according to 0.1% of the tea quality, stir evenly, and set aside; The enzyme is a mixture of cellulase and pectinase according to the enzyme activity of 1:1;

[0030] d) Vacuum microwave treatment: place the substance in step c) in a vacuum microwave oven, adjust the vacuum degree to -0.090 ~ -0.080MPa, and use pulse microwave treatment for microwaves. Under the condition of pulse microwave dose of 0.01kw / kg, the pulse...

Embodiment 2

[0041] a) Impurity removal and cleaning: select fresh green tea leaves, remove impurities such as wood chips and branches, wash the dust on the surface of the tea leaves with clean water, drain the surface water, and set aside;

[0042] b) Pulverization of tea leaves: take the tea leaves in step a) and pulverize them to a 40-mesh sieve with a pulverizer for subsequent use;

[0043] c) Adjust liquid and add enzyme: add pure water according to the material-to-liquid ratio of 1:15, adjust the pH of the tea to 5.0 after mixing evenly, stir evenly, add compound enzyme liquid according to the mass of 0.25%, stir evenly, and set aside; wherein, the compound enzyme It is prepared by mixing cellulase and pectinase according to the enzyme activity of 1:1;

[0044] d) Vacuum microwave treatment: put the substance in step c) in a vacuum microwave oven, adjust the vacuum degree to -0.090 ~ -0.080MPa, the microwave used is pulse microwave, the dose of pulse microwave is 0.04kw / kg, and the pul...

Embodiment 3

[0055] a) Impurity removal and cleaning: select fresh green tea leaves, remove impurities such as wood chips and branches, wash the dust on the surface of the tea leaves with clean water, drain the surface water, and set aside;

[0056] b) Pulverization of tea leaves: take the tea leaves in step a) and pulverize them to a 50-mesh sieve with a pulverizer for subsequent use;

[0057] c) Adjust liquid and add enzyme: add pure water according to the ratio of material to liquid 1:18, adjust the pH of tea water to 5.5 after mixing evenly, stir evenly, add compound enzyme liquid according to the mass of 0.4%, stir evenly, and set aside; wherein, the compound enzyme It is prepared by mixing cellulase and pectinase according to the enzyme activity of 1:1;

[0058] d) Vacuum microwave treatment: place the substance in step c) in a vacuum microwave oven, adjust the vacuum degree to be -0.090~-0.080MPa, the microwave used is pulse microwave, the dose of pulse microwave is 0.07kw / kg, and t...

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Abstract

The invention relates to a microwave-assisted enzymatic extraction process of tea polyphenol, wherein the process includes the steps of removing impurities from fresh tea, cleaning and crushing the tea, blending a solution and adding an enzyme, performing vacuum-microwave treatment, performing centrifugal separation, performing resin adsorption, performing secondary elution, vacuum-concentration to an eluant and freeze-drying the concentrated liquid to obtain tea polyphenol dry powder. The microwave is a pulse microwave. By means of strict control of pulse microwave dosage, pulse width and pulse period, biological effects of microwave are fully achieved, so that the activities of the enzymes are activated and the object of quickly and high-effectively extracting the tea polyphenol is achieved. The process is high in efficiency, is less in energy consumption and is less in extraction time. The tea polyphenol is high in purity and bio-activity, is green and environment-friendly, and is natural and pollution-free.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a microwave-assisted enzymatic extraction process of tea polyphenols. Background technique [0002] Tea Polyphenols (Tea Polyphenols referred to as TP), also known as tea tannin, is a general term for a class of polyhydric phenolic compounds contained in tea. Tea polyphenols are a new type of natural antioxidants, which can effectively sterilize and inhibit bacterial growth, and can also scavenge active oxygen free radicals, inhibit lipid peroxidation, and have anti-aging properties. At the same time, tea polyphenols have detoxification and anti-radiation effects, can effectively prevent radioactive substances from invading bone marrow, and can quickly excrete strontium 90 and cobalt 60 from the body. additive. It is widely used in food, medicine, agriculture, daily chemical industry and other fields. [0003] At present, the extraction and purification me...

Claims

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Application Information

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IPC IPC(8): C07G99/00
CPCY02P20/10
Inventor 金锡武
Owner HUNAN FEIXIANG JINYANG BIOTECH CO LTD
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