A kind of cationic skeleton polymer that can be used for extracting heparin and preparation method thereof

Abstract

The invention relates to a cationic skeleton polymer, its preparation method and its application in heparin extraction. Using the unique three-dimensional skeleton structure of the existing melamine sponge, the chitosan coating is coated on the skeleton, and then diethylene glycol diglycidyl ether reacts with the chitosan coating to make it cross-linked and carried on the surface. A large number of epoxy groups, and then use epoxy groups to react with diethylamine to generate tertiary amine groups, and then use diethyl sulfate for quaternization reaction, so that the surface of the skeleton is strongly hydrophilic due to the suspended cations , and through the electrostatic interaction with the sulfate anion on the heparin macromolecule to achieve efficient adsorption, it plays a key role in the process of extracting heparin from the porcine small intestinal mucosa. The advantages of using this material are: strong adsorption capacity, simplified operation, material can be recycled for many times, cost reduction and production efficiency are improved.

Description

technical field [0001] The invention relates to the technical field of bioactive substance extraction, in particular to a method for rapidly and efficiently extracting heparin by using a cationic skeleton polymer and a preparation method for the polymer material. Background technique [0002] Heparin is a natural anticoagulant drug. 10mg heparin can inhibit 500mL plasma coagulation within 4 hours. It also has the functions of clarifying plasma lipids, lowering blood cholesterol and enhancing the efficacy of anticancer drugs. It is widely used in clinical practice before and after various surgical operations. Prevention and treatment of thrombosis and blood clots, anticoagulant for preventing blood coagulation during blood transfusion and preservation of fresh blood, used for prevention and treatment of hyperlipidemia and atherosclerosis in small doses. Heparin is produced by mast cells of animal connective tissue. It is widely present in various organs and tissues of mammals...

AI Technical Summary

Problems solved by technology

[0004] The primary technical problem to be solved by the present invention is to provide a cationic skeleton polymer with a three-dimensional network structure and 100 micron through-holes for the existing problem that the pore size of the ion-exchange resin is small in the process of extracting heparin from the pig small intestine mucosa. The surface is rich in positive charges, maximizing the adsorption of negatively charged heparin molecules, avoiding the effect of the diffusion rate on the adsorption efficiency and rate, and can easily elute the adsorbed substances, realizing the repeated use of the material and improving the heparin production efficiency

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