Low-temperature quick-forming three-dimensional printing collagen silk fibroin material

A silk fibroin and three-dimensional printing technology, applied in medical science, prosthesis, etc., can solve the problems of poor three-dimensional porous network structure, poor biomechanical properties, and structural instability, and achieve good biocompatibility and mechanical properties The effect of good performance, good properties and stable structure

Inactive Publication Date: 2015-11-11
THE CHINESE PEOPLES ARMED POLICE LOGISTICS INST AFFILIATED HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ability of a single collagen to construct a three-dimensional porous network structure is poor, the structure is unstable, the biomechanical properties are poor, and the degradation rate in vivo is relatively fast.

Method used

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  • Low-temperature quick-forming three-dimensional printing collagen silk fibroin material
  • Low-temperature quick-forming three-dimensional printing collagen silk fibroin material
  • Low-temperature quick-forming three-dimensional printing collagen silk fibroin material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment 1 collagen / silk fibroin material

[0028]

[0029]

[0030] (1) Preparation of collagen solution

[0031] Tender pigskin or beef tendon is used as collagen biomaterial, cleaned and degreased with NaOH solution of pH 8.0-12, dissolved in acetic acid solution of pH 5.0-6.5 after degreasing, mixed with compound protease, collagen and The mass ratio of the composite protease is 500-1000:1, and the stirring is continued overnight;

[0032] Centrifuge, collect the supernatant, add NaCl, stir, collect the precipitate, add acetic acid, repeat the operation, repeat the centrifugation, collect the supernatant, add NaCl, stir, collect the precipitate, add the step of acetic acid 3 times, the obtained precipitate For collagen, dissolve collagen in 0.01-0.1% acetic acid solution to obtain 1-3% collagen solution.

[0033] (2) Preparation of silk fibroin solution

[0034] Take the silk, put the silk raw material into 80-120℃ Na 2 CO 3 In the so...

Embodiment 2

[0037] Example 2 Printing of Collagen / Silk Fibroin Scaffold Material

[0038](1) The solution of the biological material obtained in Example 1 is placed in the material storage of the printer; the diameter of the needle of the bioprinter is 60-400 μm, the number of needles is 6-16, the printing layer from the needle to the base is 100-300 μm, and the printing speed 2-10mm / sec, the printing gap is 100μm-400μm, and the temperature is set at -30℃-5℃.

[0039] (2) The scaffold material to be printed 60 Co sterilized and sterilized, put in 10 6 —10 8 / ml neural stem cell culture medium, cultured for 8-14 days.

[0040] (3) Rat craniocerebral injury model is prepared, and the prepared scaffold material containing cells is placed into the injury site to form local cell directional differentiation, which can be evenly distributed and can replace the biological scaffold material.

Embodiment 3

[0041] The preparation of embodiment 3 collagen / silk fibroin material

[0042]

[0043] (1) Preparation of collagen solution

[0044] Tender pigskin or beef tendon is used as collagen biomaterial, cleaned and degreased with NaOH solution of pH 8.0-12, dissolved in acetic acid solution of pH 5.0-6.5 after degreasing, mixed with compound protease, collagen and The mass ratio of the composite protease is 500-1000:1, and the stirring is continued overnight;

[0045] Centrifuge, collect the supernatant, add NaCl, stir, collect the precipitate, add acetic acid, centrifuge, collect the supernatant, add NaCl, stir, collect the precipitate, add acetic acid, repeat the operation Repeat the centrifugation, collect the supernatant, add NaCl , stirring, collecting the precipitate, and adding acetic acid for 4 times, the precipitate obtained is collagen, and the collagen is dissolved in 0.01-0.1% acetic acid solution to obtain a 1-3% collagen solution.

[0046] (2) Preparation of silk ...

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Abstract

The invention provides a low-temperature quick-forming three-dimensional printing collagen silk fibroin material. The collagen silk fibroin material is prepared by, by mass, 40-80 parts of collagen, 6-45 parts of silk fibroin, 0.5-6 parts of glycerol and 0.1-5 parts of compatilizer. Adopted materials are uniform, high in biocompatibility and mechanical performance and controllable in biodegradability and can be degraded to be nontoxic and harmless substances absorbable for human bodies in different tissues in living organisms; in-vivo degrading time can be controlled by controlling a mass ratio of components so as to promote tissue regeneration.

Description

technical field [0001] The invention relates to a low-temperature rapid prototyping three-dimensional printing collagen silk fibroin material and a preparation method and application thereof. Background technique [0002] Biomaterials are an important part of tissue engineering technology, and biodegradable materials with controllability in vivo are the development trend of tissue engineering materials in the future. Low-temperature rapid prototyping technology has gradually matured in recent years. On the basis of maintaining the biological activity of materials, this technology can quickly print out materials according to the three-dimensional parameters of the design or object under the control of computer control programs, special software, and precision motors. An object or active biological material with a three-dimensional structure. Combining the advantages of low-temperature rapid prototyping technology and the characteristics of biomaterials, developing its applic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/58A61L27/24A61L27/22
Inventor 陈旭义涂悦李瑞欣张赛汤锋武马军刘红斌杨凯
Owner THE CHINESE PEOPLES ARMED POLICE LOGISTICS INST AFFILIATED HOSPITAL
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