A microbial complex for the elimination of sulfide in sediments
A technology of complex flora and sulfide, applied in microorganism-based methods, methods of using microorganisms, microorganisms, etc., can solve the problems of elimination, narrow ecological niche of pure bacteria, and inability to adapt to the sediment environment, so as to reduce the adaptation process. , the effect of maintaining structure and composition
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Embodiment 1
[0025] Example 1: Screening, identification and genetic stability of microbial complexes for sulfide elimination in sediments
[0026] (1) Screening of microbial complex flora for sulfide elimination in sediments
[0027] 1) Collect the surface sediments within 10cm of depth in offshore aquaculture areas, and inoculate at a volume percentage of 10% to make a bacterial suspension:
[0028] In an Erlenmeyer flask, inoculate sterilized old seawater with a volume percentage of 10% of the collected surface sediment within a depth of 10 cm in an offshore culture area. Seal the Erlenmeyer flask with aluminum foil and place it on a constant temperature shaker at 30°C and shake at 120r / min for 30min to fully release the bacteria in the sediment. After shaking, let it stand for 30 minutes until the sediment re-precipitates, and the supernatant is the bacterial suspension;
[0029] 2) Inoculate the bacterial suspension in the above step 1) with an inoculum size of 10% by volume percent...
Embodiment 2
[0053] Embodiment 2: This study microbial compound bacterial agent is to the removal effect of sulfide
[0054] 1) Quickly thaw the microbial complex flora stored at -80°C in step 6) of the screening of microbial complex flora for sulfide elimination in sediments determined in Example 1 in a 30°C water bath;
[0055] 2) Inoculate the microbial complex flora after thawing in step 1) in an inoculum size of 10% by volume percentage in an inorganic salt medium with an initial concentration of sulfide of 1000 mg / l, in an anaerobic bottle at 30° C. , 150r / min constant temperature shaking culture. Among them, the inorganic salt medium is: K 2 HPO 4 : 1.2g / l, KH 2 PO 4 : 1.2g / l, NH 4 Cl: 0.4g / l, MgCl 2 : 0.2g / l, ferric citrate: 0.01g / l, NaHCO 3 : 2g / l, Na 2 S 2 o 3 ·5H 2 O: 3.875g / l, prepared with sterilized aged seawater.
[0056] At the same time, the microorganisms identified in Example 1 were selected for specific strains (Vibrio mimeticus, Desulfovibrio, Pseudomonas h...
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