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Preparation method of fibrous ring inter-vertebral disk tissue engineering scaffold

A tissue engineering scaffold and annulus fibrosus technology, which is applied in medical science, prosthesis, etc., can solve the problems of low simulation degree of fibrous annulus scaffold structure, lack of mechanical properties, uncontrollable degradation rate, etc., and achieve good biocompatibility , low price, and the effect of improving strength

Active Publication Date: 2015-11-18
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is that the simulation degree of the existing fiber ring support structure is not high, the degradation rate is uncontrollable, and the mechanical properties do not have anisotropy, etc.

Method used

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  • Preparation method of fibrous ring inter-vertebral disk tissue engineering scaffold
  • Preparation method of fibrous ring inter-vertebral disk tissue engineering scaffold
  • Preparation method of fibrous ring inter-vertebral disk tissue engineering scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Preparation of spinning stock solution: add konjac glucomannan to distilled water and stir for 15 minutes at a rotational speed of 1400r / min, then seal it and place it in a 700W microwave oven for 22 seconds, take it out and cool it to room temperature to obtain spinning Silk stock solution, the concentration of konjac glucomannan in the spinning stock solution is 20g / L;

[0035] (2) Wet spinning: Pour the spinning stock solution into the spinning equipment (60mL medical syringe), put the nozzle of the spinning equipment into the solidified liquid, and the spinning equipment moves according to the set trajectory during the spinning process, The sprayed spinning stock solution is solidified into filaments in the solidification solution, and with the accumulation of filamentous fibers, it is necessary to replace the new solidification solution in time (the solidification solution is a mixed solution of ethanol and glycerol, wherein, in the mixed solution The volume fr...

Embodiment 2

[0039] (1) Preparation of spinning stock solution: add gelatin to distilled water and let it stand for 10 hours, then heat it in a water bath to 55°C, and stir well until the gelatin is completely dissolved; add konjac glucomannan powder and stir, the stirring speed is 1300r / min, and the time is 10min until the konjac glucomannan powder is fully mixed with the gelatin solution, sealed and placed in a microwave oven with a power of 700W to heat for 24s. After the heating is completed, take it out and cool it to room temperature to obtain the spinning stock solution; the concentration of konjac glucomannan in the spinning stock solution is 30g / L, gelatin concentration is 25g / L;

[0040] (2) Wet spinning: Pour the spinning stock solution into the spinning equipment (60mL medical syringe), put the nozzle of the spinning equipment into the solidified liquid, and the spinning equipment moves according to the set trajectory during the spinning process, The sprayed spinning stock solu...

Embodiment 3

[0044] (1) Preparation of spinning stock solution: Add nano-hydroxyapatite powder into distilled water, stir until the hydroxyapatite powder forms a uniform suspension state in distilled water, add konjac glucomannan powder and stir at a stirring speed of 1350r / min, the stirring time is 20min, until the konjac glucomannan powder is fully mixed with the suspension, sealed and placed in a microwave oven with a power of 700W to heat for 26s, after the heating is completed, take it out and cool it to room temperature to obtain the spinning stock solution; The concentration of glucomannan is 15g / L, and the concentration of nano-hydroxyapatite is 20g / L;

[0045] (2) Wet spinning: Pour the spinning stock solution into the spinning equipment (60mL medical syringe), put the nozzle of the spinning equipment into the solidified liquid, and the spinning equipment moves according to the set trajectory during the spinning process, The sprayed spinning stock solution is solidified into fila...

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Abstract

The invention discloses a preparation method of a fibrous ring inter-vertebral disk tissue engineering scaffold, and belongs to the application field of biomedical material technologies. The preparation method comprises the following steps: taking KGM, gelatin and nano-hap as basic raw materials; taking ethanol and glycerine as curing agents for curing according to the wet spinning technology; after curing, adopting a cross-linking agent comprising ethanol and ammonia water for preliminary cross-linking; then adopting an alkali liquor for further cross-linking; carrying out freeze drying, so as to obtain the non-isotropic fibrous ring inter-vertebral disk tissue engineering scaffold. The preparation method has the advantage that the product is high in structural simulation, anisotropy and porosity rate, optional in design of size and shape, and adjustable in degradation rate. The ring inter-vertebral disk tissue engineering scaffold prepared according to the method has a favorable application prospect.

Description

technical field [0001] The invention relates to a preparation method of an intervertebral disc annulus fibrosus tissue engineering support, which belongs to the technical application field of biomedical materials. Background technique [0002] Degenerative disc disease is a common disease in today's society. In the United States, it ranks second only to upper respiratory tract infection, and is one of the main causes of labor loss; Asian countries such as China have a higher incidence because there are more manual workers. With the development of my country's aging process, intervertebral disc degenerative disease has increasingly become a disease that seriously affects people's work and life. The emergence of tissue engineering technology provides new ideas and methods for the treatment of intervertebral disc diseases, and is expected to provide permanent replacement products for intervertebral disc repair or replacement. Among them, the regeneration and repair of the fib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/24A61L27/20A61L27/12A61L27/56
Inventor 陈希亮陈庆华颜廷亭
Owner KUNMING UNIV OF SCI & TECH
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