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Monascus ruber strain with high extracellular yellow pigment yields, method for breeding monascus ruber strain and application thereof

A technology of Monascus and Monascus red, which is applied in the field of microbial strain breeding, can solve the problems of complicated production process, limited use, unfavorable water-soluble occasions, etc., and achieve the effect of simple operation, mild cultivation conditions and less investment

Active Publication Date: 2015-11-18
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, most of the red yeast yellow pigments are intracellular fat-soluble yellow pigments, the production process is relatively complicated, and it is not conducive to use in water-soluble occasions
Chemical transformation methods are usually used in production to change its structure and color development properties to become water-soluble pigments, but strictly speaking, after transformation, it becomes a non-natural pigment, and its use has been questioned and restricted.

Method used

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  • Monascus ruber strain with high extracellular yellow pigment yields, method for breeding monascus ruber strain and application thereof
  • Monascus ruber strain with high extracellular yellow pigment yields, method for breeding monascus ruber strain and application thereof
  • Monascus ruber strain with high extracellular yellow pigment yields, method for breeding monascus ruber strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Obtaining of Monascus strains with high production of extracellular yellow pigment

[0051] (1) Preparation of spore suspension: inoculate the original Monascus ruber strain (Monascus ruber GIM3.240, purchased from Guangdong Microbial Culture Collection Center) on a PDA slant, and after culturing at 30°C for 3 days, wash the slant of the strain with 5 mL of normal saline. Disperse with glass beads, filter out mycelium with 4 layers of sterile lens tissue, and make 1×10 spore-containing 5 individual / mL suspension.

[0052] (2) Ultraviolet mutagenesis: each petri dish with a diameter of 7 cm is loaded with 3 mL of the suspension prepared in step (1); the petri dish is placed at a vertical distance of 30 cm from a 15W ultraviolet lamp, and irradiated for 5 min under magnetic stirring to carry out UV radiation mutagenesis.

[0053] (3) Plate separation and screening: Take 0.5 mL of the bacterium solution after the mutagenesis, add 4.5 mL of sterile water to dilu...

Embodiment 2

[0056] Example 2 Utilize the Monascus strain of high-yield extracellular yellow pigment to prepare Monascus yellow pigment by conventional method

[0057] (1) Preparation of seed liquid: Inoculate the plate seeds of the original strain (MonascusruberGIM3.240) and mutant strain (MonascusruberWQ15) streaked on the PDA plate into sterilized 50mL seed medium (glucose 1g, yeast extract powder 0.15g) , fish meal peptone 0.5g, KCl 0.025g, KH 2 PO 4 0.2g, FeSO 4 ·7H 2 (00.5mg, be fixed to 50mL with distilled water, pH is natural) in carrying out culture proliferation, inoculum size is 5 single bacterium colony of cultivating 6 days, is placed in shaker control 180rpm and cultivates 30h, makes seed culture medium have mature spore, obtains seed liquid.

[0058] (2) Fermentation culture: inoculate the seed liquid into 25mL basic fermentation culture liquid (glucose 1.25g, (NH 4 ) 2 SO 4 0.125g, KH 2 PO 4 0.125g, MgSO 4 ·7H 2 O0.0125g, KCl0.0125g, FeSO 4 ·7H 2 O0.25mg, ZnSO ...

Embodiment 3

[0061] Example 3 Preparation of Monascus Yellow Pigment by Feeding Method Using Monascus Strain with High Production of Extracellular Yellow Pigment

[0062] The high-yield extracellular yellow pigment MonascusruberWQ15 and the original strain MonascusruberGIM3.240 were fermented and cultivated according to the method of Example 2. The difference from Example 2 was that after the third day of fermentation, 1.25g of glucose was added to make the fermentation culture Glucose concentration in the base rises to about 60g / L, and the fermentation is continued to 6 days, and the color value and hue of the extracellular fermentation liquid are measured according to the method in Example 2.

[0063] The result is as image 3 As shown, the absorbance at 350, 470, and 510nm of the mutant strain's fermented extracellular liquid was measured by an ultraviolet spectrophotometer to be 80.40, 4.14, and 1.00 AU / mL, respectively, and the yellow hue of the fermented extracellular red yeast rice ...

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Abstract

The invention discloses monascus ruber strain with high extracellular yellow pigment yields, a method for breeding the monascus ruber strain and application thereof. The monascus ruber strain is named as monascus ruber WQ15 and is preserved in the China General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms of the institute of microbiology of the Chinese Academy of Science in the No.3 yard on the No.2 Beichen West road of the Chaoyang district of Beijing on 2 July, 2015, and a preservation number of the monascus ruber strain is CGMCC No.10910. The monascus ruber strain with the high extracellular yellow pigment yields is obtained from riginal strains by means of ultraviolet mutation breeding. The monascus ruber strain with the high extracellular yellow pigment yields, the method and the application have the advantages that color level of extracellular yellow pigment of the monascus ruber strain is upgraded by 72% as compared with original strains by means of conventional liquid fermentation; the color level of the extracellular yellow pigment of the monascus ruber strain can be upgraded by 7.04 times as compared with the original strains if the monascus ruber strain is refilled with 300g / L of glucose during fermentation, and accordingly the monascus ruber strain can be used for producing the extracellular yellow pigment.

Description

technical field [0001] The invention belongs to the technical field of microbial strain breeding, in particular to a Monascus strain with high extracellular yellow pigment production and its breeding method and application Background technique [0002] Monascus, as a filamentous fungus, belongs to the kingdom Fungi, phylum Ascomycota, class Ascomycetes, order Phytomycota, and family Monascus. Monascus pigment is a series of natural pigments of polyketides produced by the secondary metabolism of Monascus fungus. It has been widely used as a food additive in beverages, sauces, edible oils, bread, pastries and other fields in China, Korea and Japan. It has a history of thousands of years. At present, the red yeast rice pigments used in commercial products are generally mixed pigments, which are mainly divided into three categories: red, orange, and yellow. Since the Monascus pigments fermented by Monascus in liquid state are mainly intracellular pigments, these pigments are e...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N13/00C12N15/01C12P17/18C12R1/645
Inventor 吴振强陈功汪梅花
Owner SOUTH CHINA UNIV OF TECH
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