Water quality detecting method and micro-fluidic chip special for water quality detecting method

A technology of microfluidic chips and microchannels, which is applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of high consumption of medicines, poor portability of instruments, troublesome waste liquid treatment, etc., so as to improve participation and experience, and facilitate the public The effect of simple use and detection method

Active Publication Date: 2015-12-02
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, the portability of the instrument is not good, or the performance of the portable photometer is not high (for example, lack of ultraviolet range, etc.), when testing the water quality in the field, it is necessary to take enough water samples to preserve and package, and then bring them back to the laboratory for further analysis; The operation process of the color method is relatively complicated, and it takes half an hour to start and warm up the equipment (mainly referring to the spectrophotometer), which affects the efficiency of the experimental analysis; finally, the consumption of reagents is also relatively large, and only one index can be measured at a time. relatively low
[0005] Portable photometers of commercial brands (such as Hach, WTW, etc.) adopt the standard colorimetric tube method, which can measure a variety of water quality indicators. The disadvantage is that it needs to carry a battery or a portable power supply, and the volume is relatively large and the cost

Method used

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  • Water quality detecting method and micro-fluidic chip special for water quality detecting method
  • Water quality detecting method and micro-fluidic chip special for water quality detecting method
  • Water quality detecting method and micro-fluidic chip special for water quality detecting method

Examples

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Effect test

Embodiment 1

[0053] Example 1. Microfluidic chip for detecting nitrite content in water samples

[0054] Such as figure 1 As shown, the microfluidic chip of the present invention includes an upper PDMS cover sheet 1 with a thickness of 3 mm, a PDMS middle layer 2 with a thickness of 5 mm, and a glass sheet 3 with a thickness of 0.17 mm, which are sequentially stacked and sealed. A cylindrical sample injection hole 4 with a diameter of 5 mm is provided on the upper PDMS cover slip 1 . The two ends of the PDMS intermediate layer 2 are respectively provided with a cylindrical sampling pool 5 with a diameter of 5 mm and a cylindrical raffinate chamber 7 with a diameter of 5 mm, the sampling pool 5 and the sampling pool The holes 4 are connected and their positions overlap, and a glass fiber filter paper 9 with a diameter of 5 mm is laid in the sampling pool 5 . Such as figure 2 As shown, a main micro-channel 8 with a length of 3 cm, a width of 0.2 mm, and a height of 0.5 mm is connected be...

Embodiment 2

[0068] Embodiment 2, using the microfluidic detection chip in embodiment 1 to detect the content of nitrite in the water sample

[0069] Using the microfluidic chip in Example 1, detect the content of nitrite in the water sample according to the following steps:

[0070](1) Sampling: Take out the vacuum-packaged water quality detection microfluidic chip, add 200 μL of sodium nitrite standard aqueous solution with a mass concentration of 0.1 mg / L sodium nitrite in the sampling pool; after the standard solution fills the sampling pool, use a needle to Break the sealed plastic film of the sampling pool, the standard solution will automatically enter the sampling pool, the standard solution will enter the reaction chamber along the main micro-channel and the branch micro-channels on both sides, the nitrite in the standard solution and the The chromogen N-(1-naphthyl)-ethylenediamine undergoes a color reaction.

[0071] (2) Taking pictures: the above-mentioned microfluidic chip fo...

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Abstract

The invention discloses a water quality detecting method and a micro-fluidic chip special for the water quality detecting method. The micro-fluidic chip comprises an upper-layer cover plate (1), a middle layer (2) and a lower-layer gasket (3) which are overlapped in sequence and in seal fit, wherein a sample feeding hole (4) is formed in the upper-layer cover plate (1); the middle layer comprises a sample feeding tank (5), a reaction chamber (6) and a residual liquid chamber (7); the sample feeding tank (5) and the sample feeding hole (4) are communicated and are corresponding in position; the sample feeding tank (5) and the residual liquid chamber (7) are communicated through a main micro channel (8); the main micro channel (8) extends outwards to form multiple pairs of branch micro channels (10), and the end part of each branch micro channel is communicated with the reaction chamber (6); the lower-layer gasket (3) is made of a transparent material. The micro-fluidic chip can be conveniently used by people, and facilitates the ' Internet +' popularization; a color development chip is separated from a detection device, and is easy to integrate with mobile equipment and Internet of Things; the multi-index detection can be realized.

Description

technical field [0001] The invention relates to a water quality detection method and a special microfluidic control chip thereof, belonging to the technical field of water environment monitoring. Background technique [0002] Water quality index is an important parameter used to evaluate the characteristics and functionality of water samples. According to the water quality index, the water quality can be graded and classified, and the risk can be evaluated and appropriate treatment can be carried out. At present, my country's water quality indicators have formed a relatively complete system, including surface water, groundwater, urban drainage, industrial wastewater and other fields, which are of great significance to environmental protection, water conservancy engineering, biological treatment and other fields. [0003] Water quality indicators mainly include physical indicators and chemical indicators, among which chemical indicators reflect the load state of the environme...

Claims

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Application Information

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IPC IPC(8): G01N21/25
Inventor 李冰邱勇周方明丁宇焜
Owner TSINGHUA UNIV
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