Water quality detecting method and micro-fluidic chip special for water quality detecting method
A technology of microfluidic chips and microchannels, which is applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of high consumption of medicines, poor portability of instruments, troublesome waste liquid treatment, etc., so as to improve participation and experience, and facilitate the public The effect of simple use and detection method
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Embodiment 1
[0053] Example 1. Microfluidic chip for detecting nitrite content in water samples
[0054] Such as figure 1 As shown, the microfluidic chip of the present invention includes an upper PDMS cover sheet 1 with a thickness of 3 mm, a PDMS middle layer 2 with a thickness of 5 mm, and a glass sheet 3 with a thickness of 0.17 mm, which are sequentially stacked and sealed. A cylindrical sample injection hole 4 with a diameter of 5 mm is provided on the upper PDMS cover slip 1 . The two ends of the PDMS intermediate layer 2 are respectively provided with a cylindrical sampling pool 5 with a diameter of 5 mm and a cylindrical raffinate chamber 7 with a diameter of 5 mm, the sampling pool 5 and the sampling pool The holes 4 are connected and their positions overlap, and a glass fiber filter paper 9 with a diameter of 5 mm is laid in the sampling pool 5 . Such as figure 2 As shown, a main micro-channel 8 with a length of 3 cm, a width of 0.2 mm, and a height of 0.5 mm is connected be...
Embodiment 2
[0068] Embodiment 2, using the microfluidic detection chip in embodiment 1 to detect the content of nitrite in the water sample
[0069] Using the microfluidic chip in Example 1, detect the content of nitrite in the water sample according to the following steps:
[0070](1) Sampling: Take out the vacuum-packaged water quality detection microfluidic chip, add 200 μL of sodium nitrite standard aqueous solution with a mass concentration of 0.1 mg / L sodium nitrite in the sampling pool; after the standard solution fills the sampling pool, use a needle to Break the sealed plastic film of the sampling pool, the standard solution will automatically enter the sampling pool, the standard solution will enter the reaction chamber along the main micro-channel and the branch micro-channels on both sides, the nitrite in the standard solution and the The chromogen N-(1-naphthyl)-ethylenediamine undergoes a color reaction.
[0071] (2) Taking pictures: the above-mentioned microfluidic chip fo...
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