Graphene derivative based glucose oxidase electrode and preparation method thereof

A technology of glucose oxidase and graphene, which is applied in the field of electrochemical analysis and detection, can solve the problems of poor conductivity of graphene oxide and low electrode reusability, and achieve the effects of improving detection sensitivity, facilitating reusability, and good stability

Inactive Publication Date: 2015-12-09
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

JuanTian et al. directly used graphene oxide to build electrodes for signal amplification. However, graphene oxide has a high solubility in water. In water system detection, the electrode’s reusability is low. In addi

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  • Graphene derivative based glucose oxidase electrode and preparation method thereof
  • Graphene derivative based glucose oxidase electrode and preparation method thereof
  • Graphene derivative based glucose oxidase electrode and preparation method thereof

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preparation example Construction

[0019] The preparation method of the glucose oxidase electrode based on graphene derivative, concrete steps are as follows:

[0020] Step 1, the base electrode is polished, cleaned and dried;

[0021] Step 2. Dissolve equal mass of dopamine hydrochloride and graphene oxide in Tris-HCl buffer solution at pH 8.5, disperse evenly, stir and react, then centrifuge and wash, dry to obtain graphene derivative / dopamine complex, and then compound The graphene derivative / dopamine complex solution with a concentration of 0.5 to 3 mg / mL was prepared by dissolving the dopamine;

[0022] Step 3, drop-coating the graphene derivative / dopamine complex solution obtained in step 2 on the treated conductive electrode substrate;

[0023] Step 4, use the electrode obtained in step 3 as the working electrode, Ag / AgCl as the reference electrode, the platinum electrode as the counter electrode, and the dopamine phosphate buffer solution with a concentration of 4mM and a pH value of 7.5 as the electro...

Embodiment 1

[0029] Step 1, the platinum electrodes are respectively made of Al with a particle size of 0.3 μm and 0.05 μm 2 o 3 The suspension was polished into a mirror surface on suede, then ultrasonicated with absolute ethanol and ultrapure water for 2 minutes each, and dried in a desiccator for later use;

[0030] Step 2. Dissolve 30 mg of dopamine hydrochloride in PH=8.5 Tris-HCl solution, then add graphene oxide of equal mass, disperse evenly by ultrasonic, stir and react for 4 hours, centrifuge at 14,000 rpm and 4°C for 30 minutes, remove the supernatant, and Centrifuge and wash three times with sub-distilled water, then dry the precipitate, dissolve the complex in sub-distilled water, and ultrasonically disperse to prepare a 0.5 mg / mL graphene derivative / dopamine complex solution;

[0031] Step 3, drop 10 μ L of the graphene derivative / dopamine complex solution obtained in step 2 on the treated platinum electrode;

[0032] Step 4, use the electrode obtained in step 3 as the work...

Embodiment 2

[0039] Step 1, the platinum electrodes are respectively made of Al with a particle size of 0.3 μm and 0.05 μm 2 o 3The suspension was polished into a mirror surface on suede, then ultrasonicated with absolute ethanol and ultrapure water for 2 minutes each, and dried in a desiccator for later use;

[0040] Step 2. Dissolve 30 mg of dopamine hydrochloride in PH=8.5 Tris-HCl solution, then add graphene oxide of equal mass, disperse evenly by ultrasonic, stir and react for 4 hours, centrifuge at 14,000 rpm and 4°C for 30 minutes, remove the supernatant, and Centrifuge and wash with sub-distilled water three times, then dry the precipitate, dissolve the complex in sub-distilled water, and ultrasonically disperse to prepare a 1 mg / mL graphene derivative / dopamine complex solution;

[0041] Step 3, drop 10 μ L of the graphene derivative / dopamine complex solution obtained in step 2 on the treated platinum electrode;

[0042] Step 4, use the electrode obtained in step 3 as the working...

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Abstract

The invention discloses a graphene derivative based glucose oxidase electrode and a preparation method thereof. The electrode is sequentially composed of a conductive electrode substrate, a graphene derivative/dopamine composite layer, a polydopamine layer, a nanogold array, a 4-mercaptophenylboronic acid layer, and a glucose oxidase layer. According to the invention, dopamine is employed to reduce graphene oxide partially into reduced graphene oxide to form a graphene derivative/dopamine composite layer gathering on the surface of the conductive electrode substrate, also electropolymerization of a layer of polydopamine is carried out on the surface of the graphene derivative/dopamine composite layer, self-assembly of nanogold particles is performed on the platform, and then 4-mercaptophenylboronic acid is loaded to the nanogold array so as to fix glucose oxidase. The glucose oxidase electrode has the advantages of simple preparation method, low detection limit, high sensitivity and high enzyme bonding strength, and can realize sensitive detection of glucose.

Description

technical field [0001] The invention belongs to the technical field of electrochemical analysis and detection, and in particular relates to a graphene derivative-based glucose oxidase electrode and a preparation method thereof. Background technique [0002] Diabetes mellitus, a metabolic disease characterized by hyperglycemia due to insulin deficiency, is one of the leading causes of death and disability. Diagnosis and control of diabetes requires constant monitoring of blood glucose levels, so thousands of diabetics need to check their blood sugar every day. How to carry out reliable and close blood sugar control is still a topic worthy of research. Among them, the glucose electrochemical biosensor plays an important role in blood glucose monitoring, and the current-type enzyme electrode based on the glucose oxidase immobilized on the electrode is widely used in the research of the glucose electrochemical biosensor. [0003] In recent years, glucose detection has graduall...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 单丹孙文秀袁培新
Owner NANJING UNIV OF SCI & TECH
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