Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of piperine in the preparation of drugs for preventing and treating sepsis

A sepsis and piperine technology, applied in the application field of piperine in the preparation of anti-septic drugs, can solve the problems of weakening the effect of anti-infection treatment, and achieve the effects of enhancing activity, preventing apoptosis, and preventing sepsis

Active Publication Date: 2017-12-12
JINAN UNIVERSITY
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the abuse of antibiotics, many bacteria have developed drug resistance, which weakens the effect of anti-infective treatment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of piperine in the preparation of drugs for preventing and treating sepsis
  • Application of piperine in the preparation of drugs for preventing and treating sepsis
  • Application of piperine in the preparation of drugs for preventing and treating sepsis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Piperine upregulates the activity of intracellular mTORC1

[0042] Add different concentrations (40, 80, 160 μmol / L) of piperine to the cell lines cultured in 6-well plates (prostate cancer cell line LNCaP, human cervical cancer cell line HeLa, mouse macrophage cell line RAW 264.7) and incubate for 2 hours , the protein after cell lysis was collected, and the phosphorylation of mTORC1 downstream substrates p70S6K and 4E-BP1 was detected by Western blot method.

[0043] The results showed that piperine pretreated LNCaP( figure 1 ), HeLa( figure 2 ) and RAW 264.7 cells ( image 3 ), up-regulated the phosphorylation of p70S6K and 4E-BP1 proteins in a dose-dependent manner (concentrations of 40, 80, and 160 μmol / L, respectively), without affecting the expression of p70S6K and 4E-BP1 proteins in cells. Figure 1-3The histogram respectively counts the ratio of p-p70S6K / p70S6K to p-4E-BP1 / 4E-BP1 in the three types of cells, *P<0.05 (the difference is statistically signific...

Embodiment 2

[0045] Piperine acts as an enhancer of mTORC1 during nutrient deprivation (low intracellular mTORC1 activity)

[0046] 40 μmol / L piperine was added to serum-starved HeLa cell culture medium (serum-free EBSS or DMEM medium), and Western blot was used to detect the phosphorylation of p70S6K, a downstream substrate of mTORC1, to evaluate the activity of mTORC1.

[0047] It was found that after HeLa cells were starved by serum-free EBSS, the phosphorylation level of p70S6K (i.e. the activity of mTORC1) was low, and whether the EBSS medium contained glucose (Glc) or piperine did not affect the expression of p70S6K. Phosphorylation level ( Figure 4 ), indicating that the effect of piperine on mTORC1 is independent of glucose. However, serum-free DMEM medium can activate the phosphorylation of p70S6K, and piperine can significantly enhance the phosphorylation of p70S6K induced by DMEM (***P<0.001). The results indicated that the upregulation of mTORC1 by piperine may be related to...

Embodiment 3

[0049] When the activity of mTORC1 in cells is low, piperine combines with glutamine and leucine to activate the mTORC1 signaling pathway

[0050] Add 40 μmol / L piperine, 0.8 mmol / L L-leucine (L-Leucine, Leu) and 2 mmol / L L-glutamine (L-Glutamine, Gln) (the same below) to serum-starved HeLa cells ( Figure 5-6 ) and mouse peritoneal macrophages ( Figure 7 ) medium.

[0051] The results showed that phosphorylation of p70S6K (indicative of mTORC1 activation) is dependent on the presence of intracellular Gln and Leu. Such as Figure 5 As shown, the activity of mTORC1 was inhibited in HeLa cells under serum and amino acid starvation (50 min) conditions (the first lane). Gln itself cannot activate mTORC1. Leu can slightly upregulate mTORC1 due to the presence of a small amount of Gln in cells after a short period of starvation. The addition of piperine significantly up-regulated the Leu-activated mTORC1 activity (*P<0.01).

[0052] Figure 6 It was further shown that piper...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
melting pointaaaaaaaaaa
Login to View More

Abstract

The invention discloses application of piperine to the preparation of drugs for preventing and treating septicemia. Piperine can strongly enhance the leucine-induced activation of mTORC1. Piperine can prevent bacterial abdominal infection and further prevent septicemia, and can also enhance the functions of macrophages, including bacterial phagocytosis and TNF-alpha and IL-6 secretion capability, to prevent bacterial infection induced macrophage apoptosis. Therefore, piperine or the combination of piperine, leucine and glutamine can enhance the mTORC1 activity of bodies and be used for treating bacterial infections and septicemia.

Description

technical field [0001] The invention belongs to the field of medicines, and in particular relates to the application of piperine in the preparation of medicines for preventing and treating sepsis. Background technique [0002] Piperine (Piperine) is a natural compound extracted from plants of the family Piperaceae, and its molecular formula is C 17 h 19 NO 3 ; The chemical name is (E,E)-1-[5-(1,3-benzodioxolan-5-yl)-1-oxo-2,4-pentadienyl]-piperidine. White crystalline powder. The melting point is 130-133°C. Soluble in acetic acid, benzene, ethanol and chloroform, slightly soluble in ether. Its chemical structure is as follows: [0003] [0004] The theory and practice of traditional Chinese medicine show that pepper enters the stomach and large intestine meridian, warms the middle and dispels cold, lowers qi and eliminates phlegm. It can be used for stomach cold and vomiting, abdominal pain and diarrhea, loss of appetite, epilepsy and excessive phlegm. Simply put,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4525A61P31/04
Inventor 欧阳东云何贤辉潘浩徐丽慧黄美云查庆兵施资坚
Owner JINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com