A poisonous microcarbon algae crack algae and its separation method and application

A technology of cyanophage and Microcystis, which is applied in the field of pyrolysis and separation of toxic Microcystis cyanophage, can solve the problems of consuming a lot of manpower and material resources, and chemical agent pollution, and achieve rapid lysis, strong specificity, and high cultivation conditions simple effect

Active Publication Date: 2018-05-18
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the physical method is quick to control algae, it needs a lot of manpower and material resources
The chemical method mainly uses chemical agents to control algae, and its disadvantage is that the chemical agents will cause pollution in the water body

Method used

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  • A poisonous microcarbon algae crack algae and its separation method and application
  • A poisonous microcarbon algae crack algae and its separation method and application
  • A poisonous microcarbon algae crack algae and its separation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of cyanophage MaSSC-P

[0027] The water sample was collected from Ningbo Bang Cultural Park, Ningbo City, Zhejiang Province; the host algae was Microcystis aeruginosa M. aeruginosa FACHB-925, the specific preparation method is as follows:

[0028] (1) Isolation of cyanophages

[0029] The collected surface water samples containing toxic Microcystis lysed cyanophages were filtered through medium-speed filter paper, 0.45 μm and 0.22 μm nitrocellulose filter membranes; the filtrate was mixed with Microcystis aeruginosa in logarithmic growth phase by volume Infection mixture at a ratio of 1:4; at the same time, the water sample (without cyanophage particles) filtered through a tangential flow 10KD membrane was infected with Microcystis aeruginosa in the logarithmic growth phase at the same ratio as a control; 2000 lx, the temperature is 25 ℃, and the light-dark cycle is 12h:12h; the yellowed culture solution is continuously subcultured until the cyanophage...

Embodiment 2

[0038] Morphological Observation of Lysing Cycphages of Microcystis aeruginosa

[0039] Take the cyanophage isolated and purified in Example 1 on the copper grid, negatively stain with 2% uranyl acetate solution (w / v), and then observe the cyanophage under an electron microscope (Hitachi H-7650). form.

[0040] The result is as figure 1 As shown, the cyanophage has a regular polyhedral head structure and a slender tail, the diameter of the head is about 85nm, and the length of the tail is about 250nm.

[0041] The purified cyanophages were infected and mixed with exponentially growing Microcystis aeruginosa, and the algal plaque experiment was carried out, and the algal plaques with uniform size and shape could be obtained, without halos around, and clear and regular edges.

Embodiment 3

[0043] Nucleic acid type identification of cyanophage genome

[0044] Take the cyanophage particles separated and purified in Example 1, and after extracting the nucleic acid, use DNase I, Rnase A and S1 single-stranded nuclease to act on the cyanophage genome nucleic acid respectively. The action spectrum is as follows figure 2 As shown in the figure (Swimming lane 1: Untreated nucleic acid, Swimming lane 2: Dnase I enzyme treatment, Swimming lane 3: Rnase A enzyme treatment, Swimming lane 4: S1 single-strand enzyme treatment) the cyanophage can be detected by Dnase I, single-strand Nuclease digestion, but cannot be digested by RNase A enzyme, and then it is concluded that cyanophage MaSSC-P is a virus particle containing single-stranded DNA (ssDNA); according to the "Virus Classification- According to the Eighth Report of the International Committee on Taxonomy of Viruses, the cyanophage does not belong to Myoviridae, Longoviridae and Brachyviridae, and should represent a n...

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Abstract

The invention discloses a toxic Microcystis lysing cyanophage and its separation method and application. On May 25, 2015, it was preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the preservation number was CGMCC No.10598. The purified cyanophage suspension was added to the Microcystis bloom sample, and the water sample contained Microcystis has a specific cracking effect, and it has a cracking effect on Microcystis aeruginosa, Microcystis whitworthi and Microcystis viridans of the genus Microcystis. The advantage is that it can crack fresh water safely, efficiently and quickly Toxic Microcystis.

Description

technical field [0001] The invention relates to a toxic Microcystis lytic cyanophage, in particular to a toxic Microcystis lytic cyanophage and its separation method and application. Background technique [0002] In recent years, human economic activities have become more and more serious, and various domestic sewage and production wastewater have been discharged into slow-flowing water bodies such as lakes, estuaries, and bays, causing excessive nutrients such as nitrogen and phosphorus in the water body to become eutrophic. The algae that causes is multiplied in great numbers, and the occurrence of " water bloom " becomes more and more serious, and wherein blue-green algae becomes the main alga that causes water bloom outbreak. "Water bloom" is one of the most serious environmental disasters in the world today. Among them, my country is one of the countries with the most serious outbreak of water bloom. About 75% of the lakes are facing different degrees of eutrophication ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00C12N7/02C02F3/32
Inventor 刘飞李登峰严小军刘联国吴寒华顾叶华陈梅娟
Owner NINGBO UNIV
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