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Method for preparing sea cucumber bait through solid state fermentation of mixed bacteria

A solid-state fermentation, bait technology, applied in animal feed, animal feed, application and other directions, can solve the problems of endangering food safety and human health, decreased immunity of sea cucumber, drug resistance of bacteria, etc., and achieves good product quality and easy application. And promotion, the effect of short production cycle

Inactive Publication Date: 2016-01-06
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional breeding process, in order to reduce the amount of microorganisms carried in the feed or improve the effect of disease treatment, farmers often add antibiotics to the feed. The use of a large number of antibiotics not only reduces the immunity of sea cucumber itself, but also induces drug resistance of the bacteria. Sex, but also brought serious problems such as drug residues, endangering food safety and human health

Method used

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  • Method for preparing sea cucumber bait through solid state fermentation of mixed bacteria
  • Method for preparing sea cucumber bait through solid state fermentation of mixed bacteria
  • Method for preparing sea cucumber bait through solid state fermentation of mixed bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step a, preparing fermentation bacteria: culture Saccharomyces cerevisiae with YPD medium at 30°C for 24 hours; culture Bacillus at 30°C with LB broth medium for 24 hours; cultivate lactic acid bacteria at 37°C for 48 hours with MRS medium. After the cultivation, the number of Saccharomyces cerevisiae was 10 8 cfu / mL, the number of Bacillus bacteria is 5×10 8 cfu / mL, the number of lactic acid bacteria is 2×10 8 cfu / mL;

[0029] Wherein YPD medium, broth medium and MRS medium formulations are as follows:

[0030] YPD medium: glucose 20g, peptone 20g, yeast extract 10g, distilled water 1000mL, natural pH.

[0031] LB broth medium: tryptone 10g, yeast extract powder 5g, sodium chloride 10g, distilled water 1000mL, pH6.9±0.1.

[0032] MRS lactic acid medium: glucose 20g, peptone 10g, beef extract 10g, yeast extract 5g, K 2 HPO 4 2g, sodium acetate 5g, ammonium citrate 2g, MgSO 4 0.2g, MnSO 4 0.05g, Tween-801mL, distilled water 1000mL, pH6.2~6.4.

[0033] Step b, pr...

Embodiment 2

[0045]Step a, preparing fermentation bacteria: culture Saccharomyces cerevisiae with YPD medium at 30°C for 24 hours; culture Bacillus at 30°C with LB broth medium for 24 hours; cultivate lactic acid bacteria at 37°C for 48 hours with MRS medium. After the cultivation, the number of Saccharomyces cerevisiae was 10 8 cfu / mL, the number of bacillus is 8×10 8 cfu / mL, the number of lactic acid bacteria is 5×10 8 cfu / mL; Wherein YPD medium, broth medium and MRS medium formula are identical with embodiment 1;

[0046] Step b, preparation of sea cucumber bait: 20% of sargassum powder, 20% of sea green powder, 4% of fermented soybean meal, 3% of fish meal, 4% of shrimp meal, 2% of scallop powder, 32% of degummed kelp powder, and 6% of corn meal , Broken beer yeast powder 5%, premix 4% formula to prepare sea cucumber bait, mix evenly;

[0047] Step c, inoculation: various probiotics are added according to the ratio of 4% of the bait weight, and the ratio of Saccharomyces cerevisiae:...

Embodiment 3

[0058] Step a, preparing fermentation bacteria: culture Saccharomyces cerevisiae with YPD medium at 30°C for 24 hours; culture Bacillus at 30°C with LB broth medium for 24 hours; cultivate lactic acid bacteria at 37°C for 48 hours with MRS medium. After the cultivation, the number of Saccharomyces cerevisiae was 2×10 8 cfu / mL, the number of bacillus is 8×10 8 cfu / mL, the number of lactic acid bacteria is 3×10 8 cfu / mL; Wherein YPD medium, broth medium and MRS medium formula are identical with embodiment 1;

[0059] Step b, preparation of sea cucumber bait: 20% of sargassum powder, 20% of sea green powder, 4% of fermented soybean meal, 3% of fish meal, 4% of shrimp meal, 2% of scallop powder, 32% of degummed kelp powder, and 6% of corn meal , Broken beer yeast powder 5%, premix 4% formula to prepare sea cucumber bait, mix evenly;

[0060] Step c, inoculation: various probiotics are added according to the ratio of 5% of the bait weight, and the ratio of Saccharomyces cerevisi...

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Abstract

The invention discloses a method for preparing fermented sea cucumber bait through solid state fermentation of mixed bacteria. According to the method, sea cucumber bait comprising main components including sargassum powder, sea lettuce powder, soy meal, fish meal, shrimp meal, scallop skirt meal, degummed kelp powder, corn flour and the like is used as a raw fermentation material, Saccharomyces cerevisiae, Bacillus and lactic acid bacteria are used as fermentation strains, inoculation is performed on the basis of 3%-5% of bait weight, solid state fermentation is performed on the raw material at the temperature of 25-30 DEG C, and the microbial fermented bait for sea cucumbers is obtained. The production technology is simple and easy to operate, the source of the production raw material is wide, the cost is low, popularization is easy, the production cycle is short, the product quality is good, and the method is easy to popularize and apply. The survival rate and the growth performance of the sea cucumbers can be increased when the method is applied to culture of the sea cucumbers; meanwhile, the ecological environment of a water body of a culture pond can be improved, the water can be purified, and the microbial fermented bait for the sea cucumbers is ideal and high in quality.

Description

technical field [0001] The invention relates to microbial fermentation technology, in particular to a method for preparing sea cucumber fermented bait by mixing several beneficial bacteria through solid-state fermentation. Background technique [0002] In recent years, the sea cucumber aquaculture industry has developed rapidly in the northern coastal areas of my country, has huge market potential, and has gradually become one of the important industries of marine aquaculture in my country, and has achieved remarkable economic and social benefits. In the traditional breeding process, in order to reduce the amount of microorganisms in the feed or improve the effect of disease treatment, farmers often add antibiotics to the feed. The use of a large number of antibiotics not only reduces the immunity of sea cucumber itself, but also induces drug resistance of the bacteria. Sex, but also brought serious problems such as drug residues, endangering food safety and human health. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/14A23K1/10A23K1/16A23K1/175A23K1/00
Inventor 肖珊王晗王际辉孙兴
Owner DALIAN POLYTECHNIC UNIVERSITY
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