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Method for desalinating thymopeptide alpha 1

A thymosin and desalination technology, applied in the field of desalination of thymosin α1, can solve the problems of long time consumption of ion exchange resin, small amount of sample processing, low work efficiency, etc., and achieve the effect of easy industrial implementation, low environmental pollution and low production cost

Inactive Publication Date: 2016-01-20
GL BIOCHEM SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method suitable for large-scale industrialized desalination of thymosin α1, which mainly solves the long time-consuming and troublesome pretreatment of using ion exchange resin for desalination, and the small amount of sample processing at one time, low work efficiency and low reproducibility. Relatively poor, not suitable for technical problems of large-scale industrial production

Method used

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  • Method for desalinating thymopeptide alpha 1
  • Method for desalinating thymopeptide alpha 1
  • Method for desalinating thymopeptide alpha 1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. Sample treatment: After weighing 5g of thymosin α1 and dissolving it, adjust the pH value of the solution to 7, filter through a filter membrane with a pore size of 0.45um, and collect the filtrate.

[0015] 2. Desalination

[0016] Conditions: Chromatographic column: a chromatographic column with octadecyl silica gel bonded silica gel as the stationary phase, the diameter and length of the column are: 5cm×25cm. Mobile phase: A phase: aqueous solution; B phase: methanol solution. Flow rate: 60-80ml / min. Detection wavelength: 220nm. Concentration gradient: B%: 20-60%, 30-50min.

[0017] Process: equilibrate the chromatographic column with phase B with a concentration of 5% by mass, load the sample, elute with a linear gradient, collect the target peak, and place the collected peptide solution in a collection bottle.

[0018]

[0019] 3. All the peptide solutions obtained after salt conversion were concentrated under reduced pressure to 1g / 50ml, the concentratio...

Embodiment 2

[0021] 1. Sample treatment: After weighing 40g of thymosin α1 and dissolving it, adjust the pH of the solution to 8, filter through a filter membrane with a pore size of 0.45um, and collect the filtrate.

[0022] 2. Desalination

[0023] Conditions: Chromatographic column: a chromatographic column with octadecyl silica gel bonded silica gel as the stationary phase, the diameter and length of the column are: 10cm×25cm. Mobile phase: Phase A: aqueous solution; phase B: methanol solution. Flow rate: 180-200ml / min. Detection wavelength: 220nm. Concentration gradient: B%: 20-60%, 30-50min.

[0024] Process: equilibrate the chromatographic column with phase B with a concentration of 5% by mass, load the sample, elute with a linear gradient, collect the target peak, and place the collected peptide solution in a collection bottle.

[0025]

[0026] 3. All the peptide solutions obtained after salt conversion were concentrated under reduced pressure to 1g / 50ml, the concentration ...

Embodiment 3

[0028] 1. Sample treatment: Quantitatively purify thymosin α1, the content of the solution is 5.5g, adjust the pH value of the solution to 6.5, filter through a filter membrane with a pore size of 0.45um, and collect the filtrate.

[0029] 2. Desalination

[0030] Conditions: Chromatographic column: a chromatographic column with octadecyl silica gel bonded silica gel as the stationary phase, the diameter and length of the column are: 5cm×25cm. Mobile phase: Phase A: aqueous solution; phase B: methanol solution. Flow rate: 60-80ml / min. Detection wavelength: 220nm. Concentration gradient: B%: 20-60%, 30-50min.

[0031] Process: Equilibrate the chromatographic column with phase B with a concentration of 5% by mass and load the sample. Linear gradient elution, collect the target peak, put the collected peptide solution into the collection bottle.

[0032]

[0033] 3. All the peptide solutions obtained after salt conversion were concentrated under reduced pressure to 1g / 50m...

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Abstract

The invention discloses a method for desalinating thymopeptide alpha 1 through reversed-phase high-performance liquid chromatography and mainly solves the technical problems that much time is consumed in a method for desalination through iron exchange resin, pretreatment is troublesome, only a few of samples are treated each time, the work efficiency is low, reproducibility is poor, and the method is not suitable for large-scale industrial production. According to the technical scheme, the method comprises steps as follows: 1), the pH (potential of hydrogen) of a purified solution of thymopeptide alpha 1 is adjusted to range from 6 to 8, the purified solution is filtered with a filter membrane with the specification of 0.45 mu m for standby application, gradient elution for purification is performed through a reverse phase silica gel column with octadecyl silane as a fixed phase, a water solution as an A phase and a methanol solution as a B phase, and a peptide solution reaching a target peak value is collected; the peptide solution obtained finally is subjected to rotary evaporation, concentration and freeze drying under reduced pressure, and the finished powdery peptide is obtained. The method is used for large-scale and industrial desalination of thymopeptide alpha 1.

Description

technical field [0001] The invention relates to a method for desalting thymosin α1, in particular to a method for desalting thymosin α1 in batches and industrialization. Background technique [0002] Thymosin is a mixture of biologically active polypeptides secreted by the thymus, and is an immune enhancer. It plays an important regulatory role in the body's immune system, neuroendocrine system, and reproductive system. It can improve the body's immune status, coordinate endocrine, and improve the body's resistance to bacterial and viral infections. Thymosin has been used as an adjuvant therapy for many diseases, and its curative effect is remarkable. Adding it to feed can also play a role in promoting growth. A variety of polypeptides contained in thymosin have certain medicinal effects, some of which have been used in the treatment of diseases. [0003] Thymosin α1 is a polypeptide drug for treating chronic hepatitis B and enhancing the reactivity of the immune system. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/575C07K1/34C07K1/04
CPCC07K14/57581
Inventor 徐红岩秦敬国
Owner GL BIOCHEM SHANGHAI
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