Production method of beta-glucan

Abstract

The invention discloses a production method of beta-glucan. The production method comprises the following steps of (1) schizophyllum commune activation; (2) seed activation; (3) fermentation culture; (4) coarse extraction; (5) purification. A colorless liquid culture medium prepared from 25 to 30g / L of glucose, 10 to 15g / L of sorbitol, 0.2 to 0.8g / L of monopotassium phosphate, 3 to 5g / L of epsom salt, 0.5 to 1g / L of ammonium sulphate, 0.1 to 0.5g / L of triethylamine hydrochloride, 1 to 2.2ml / L of trace element mixed solution and water is used as a fermentation culture medium for performing deep layer liquid fermentation culture on strains to obtain the colorless fermentation liquid. No pigment exists in the whole process; a decoloring step is not needed; loss due to adsorption is reduced; the problem of beta-glucan separation purification is overcome; high-purity white beta-glucan is obtained; the production method is suitable for mass industrial production.

Description

technical field [0001] The invention relates to a production method of β-glucan. Background technique [0002] Glucans are natural polysaccharides found in oats, barley, wheat, yeast and fungi, including 1,3-glucan, 1,4-glucan (with branched chains) and 1,6-glucans (unbranched), they may be alpha or beta. A large number of studies have shown that β-glucan is a good immune enhancer, which can effectively stimulate immune cells, not only enhance the specific immune response of the organism, but also improve the non-specific immune response. In addition, β-glucan can also enable organisms to resist infection by bacteria, fungi, viruses and parasites, and inhibit the growth of tumors, and has been well clinically applied abroad. Due to the huge application value of β-glucan in biology, medicine, health care and food, how to effectively obtain high-purity β-glucan, especially the biologically active β-glucan that can meet the injection standard Dextran has become a key directi...

AI Technical Summary

Problems solved by technology

Although the raw material cost of the first method is low, the extraction process is complicated and inefficient, and the obtained β-glucan has low branching degree, small molecular weight, poor water solubility and structural stability, and its application value is greatly reduced; although the obtained β-glucan obtained by the second method - The branching degree, molecular weight, water solubility and structural stability of dextran are relatively high, but the source cost of mushrooms is high, the cycle is long, the production space is large, the output is low, the post-processing of the product is difficult, and impurities are easy to remain; the third method The cycle is relatively short, and the branching degree, molecular weight, water solubility and other properties of the product can be selected by selecting different strains and controlling the fermentation conditions, but it takes a lot of research to determine the optimal conditions for fermentation

[0004] However, no matter which of the above methods, a large amount of pigments with complex components will be produced in the production process, and the decolorization step must be performed to improve the purity and product quality

Taking into account many factors such as maintaining the biological activity of β-glucan, the cycle should not be too long, the environment is friendly and the use of too many organic solvents should not be used, and the production cost should be low. At present, the only practical decolorization method is to use activated carbon or resin adsorption.

However, the principles of these two methods are all physical adsorption, and the product β-glucan will be adsorbed at the same time when the pigment is removed, so that the loss rate of the product is very large, and the yield is greatly reduced.