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Method of extracting proteins and amino acids from enzymolysis waste liquid of porcine small intestinal mucous membrane

A technology of waste liquid and acetic acid, which is applied in animal feed, animal feed, application, etc., can solve the problems of difficult precise control, difficult to meet discharge standards, and small processing unit area, so as to increase revenue, save auxiliary materials, and reduce consumption costs. Effect

Inactive Publication Date: 2016-01-27
叶青理
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  • Claims
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AI Technical Summary

Problems solved by technology

[0002] In the biochemical industry, animal tissues are used as raw materials, such as intestinal mucosa, cartilage, etc., and after heparin sodium or chondroitin sulfate is extracted by enzymatic hydrolysis or salt hydrolysis, the waste liquid contains oil, fiber, protein, and small peptides. Proteins, amino acids and other components are usually treated with bacteria. Because the salinity in the water is sometimes too high, sometimes it inhibits bacterial reproduction or the production volume is unstable. As a result, the unit area treated by this method is relatively small, and the product cannot be recycled. Fine control is difficult
It is difficult to recycle the waste liquid after treatment in a slightly larger plant or discharge it up to standard

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] Preparation of materials: 1000 kg each of 1% chitosan acetate cellulose solution and 1% sodium hydroxide konjac phosphate sodium solution were prepared 12 hours in advance.

[0021] 1. Flocculation: collect about 100 tons of waste liquid after producing heparin sodium with pig small intestine mucosa in a stirred container, about 15,000 small intestines, at this time, the temperature is about 45°C, slowly add 1% chitosan acetate fiber under stirring 300-500 kg of plain solution, stir well, then add 300-500 kg of alkaline sodium konjac phosphate solution, stir while adding until evenly dispersed, about 3-5 minutes, let it stand for 4-12 hours to settle, then from the bottom The upper layer liquid is discharged from 0.8 meters above, and the lower layer sediment is introduced into the pressure filter bag for filtration with a sewage pump.

[0022] 2. Pressure filtration: After 30 minutes to 2 hours of filtration, filter the bag dry, pour out the solids and dry them to obta...

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Abstract

The present invention relates to a method of extracting proteins and amino acids from enzymolysis waste liquid of a porcine small intestinal mucous membrane. The method belongs to a sewage treatment industry engineering technology in chemical and biological fields. The purpose of the invention is to seek a method which not only can process biochemical waste liquid and recycle feed-grade proteins and amino acids to turn waste into wealth, but also can recycle reuse water and make sewage meet the environmental protection requirements, i.e., up-to-standard discharging. The method has remarkable advantages that proteins and lipids are precipitated by two food-grade celluloses from water through flocculation, the product can be used as a feed; microfiltration and nanofiltration are adopted to remove various substances with molecular weight greater than 300 from water; amino acids are adsorbed by resins; and activated carbon is used to deodorizing so as to make the saline solution can be recycled and largely reduce the COD value of the sewage. Moreover, the method can process all sewage with the similar properties, has good investment prospects, operability and economical efficiency, and is an ideal choice for processing the present type of sewage.

Description

Technical field: [0001] The invention belongs to the sewage treatment engineering technology of environmental protection in the field of biochemical industry. Specifically, it is to use food-grade fiber to treat waste liquid containing protein and amino acid by physical and chemical methods, recover protein and amino acid products from it, and turn waste into treasure, so that waste liquid can be recycled or discharged up to standard. method. Background technique: [0002] In the biochemical industry, animal tissues are used as raw materials, such as intestinal mucosa, cartilage, etc., and after heparin sodium or chondroitin sulfate is extracted by enzymatic hydrolysis or salt hydrolysis, the waste liquid contains oil, fiber, protein, and small peptides. Proteins, amino acids and other components are usually treated with bacteria. Because the salinity in the water is sometimes too high, sometimes it inhibits bacterial reproduction or the production volume is unstable. As a ...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23J1/16
Inventor 叶青理
Owner 叶青理
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