Preparation method for acellular xeno-dermal matrix

Abstract

The invention relates to a preparation method for an acellular xeno-dermal matrix. Fresh salted pigskin is employed as a raw material, physical, chemical and biochemical combination methods are employed, the pigskin is subjected to a series of treatments of degreasing, alkaline swelling, alkaline swelling elimination, keratinase processing, secondary enzyme treatment and the like, and an acellular xeno (pig)-dermal matrix with excellent performances and wide purposes is prepared and obtained. In the method, keratinase processing and secondary enzyme treatment are employed, the method has low environmental pollution, and the acellular xeno (pig)-dermal matrix processed through the method has advantages of complete collagen three-dimensional structure, good moisture permeability, high porosity and excellent mechanical properties. The acellular xeno-dermal matrix can be applied in the burn and plastic surgery fields.

Description

technical field [0001] The invention relates to the field of bioengineering, more specifically, to a method for preparing heterogeneous (pig) acellular dermal matrix Background technique [0002] Acellular matrix (acellular dermal matrix, ADM) is obtained by using physical, chemical, biological and other methods to completely remove the epidermis and cells containing antigenic components in the skin, leaving only the extracellular matrix containing collagen in the dermis. It has wound coverage , Tissue defect filling, guiding tissue regeneration and so on. Because ADM has no cellular components at all and low immune activity, it generally does not induce immune responses. However, ADM retains the three-dimensional structure of normal collagen and the extracellular matrix containing collagen scaffolds in the dermis. These components can support the growth of the split skin and are also conducive to the adhesion and proliferation of seed cells. Therefore, the acellular matrix...

AI Technical Summary

Problems solved by technology

[0003] Porcine acellular dermal matrix (PADM) prepared by traditional methods has the disadvantages of incomplete cell removal, poor permeability, low porosity, and low tensile strength.

The Chinese patent No. CN1387923 discloses a heterogeneous acellular dermal scaffold and its preparation method. The scaffold is obtained by treating suckling pig skin with trypsin and glutaraldehyde. The dermal scaffold treated by this method has tensile strength High and good biocompatibility, but its permeability is poor, the wound is prone to fluid accumulation, gas accumulation, slow vascularization, and glutaraldehyde is toxic to cells, which is potentially dangerous

The Chinese patent with the patent number CN1343523 discloses a microporous allogeneic or heterogeneous acellular dermal substitute that can be used for wound repair, using mechanical or laser methods to regularly make dense penetrating holes on the acellular dermis. Although it can solve the shortcomings of poor permeability of acellular dermal substitutes, too large or too dense pore size will increase the exposure of antigenic sites and the chances of antigen-presenting cells contacting ADM, resulting in poor cell compatibility and a certain degree of disrupt the structure of the collagen scaffold

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