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Method using streptomyces lavendulae fermentation to produce alpha-glucosidase inhibitors

The technology of Streptomyces lilacinus and glucosidase is applied in the field of microbial fermentation to achieve the effects of improving the formation of hyperglycemia, convenient operation and implementation, and high production efficiency

Inactive Publication Date: 2016-01-27
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] The above patents are all about the extraction of natural Chinese herbal medicines or the preparation of α-glucosidase inhibitors by chemical synthesis technology. At present, there is no document disclosing the method of producing α-glucosidase inhibitors by fermentation of Streptomyces lilacinus griseus

Method used

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  • Method using streptomyces lavendulae fermentation to produce alpha-glucosidase inhibitors
  • Method using streptomyces lavendulae fermentation to produce alpha-glucosidase inhibitors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～8

[0036] (1) Activation and preservation of strains

[0037] Streptomyces lilacinus ( Streptomyces lavendulae ) UN-8 was inoculated on a solid slant medium, cultured at a constant temperature of 30°C for 3 days, and stored at 4°C for a short period of time. The concentration components of the solid slant medium prepared with distilled water are: soluble starch 20g / L, KNO 3 0.5g / L, NaCl0.5g / L, K 2 HPO 4 1.0g / L, MgSO 4 ·7H 2 O0.5g / L, FeSO 4 ·7H 2 O0.01g / L, agar 20g / L, pH value 7.2.

[0038] (2) Preparation of seed solution

[0039] Primary seed preparation: put 2.0cm on the above slope 2 The bacterial lawn was inserted into a 500mL Erlenmeyer flask containing 100mL of liquid seed medium, the shaking table was rotated at 160rpm, and cultured at a constant temperature of 30°C for 36h; secondary seed preparation: the primary seed liquid was inserted into 600mL of liquid seed medium In a 3.0L Erlenmeyer flask, the inoculum amount was 2% (v / v), the shaker rotated at 160rpm, ...

Embodiment 9～16

[0045] (1) Activation and preservation of strains

[0046] Streptomyces lilacinus ( Streptomyces lavendulae ) UN-8 was inoculated on a solid slant medium, cultured at a constant temperature of 30°C for 3 days, and stored at 4°C for a short period of time. The concentration components of the solid slant medium prepared with distilled water are: soluble starch 20g / L, KNO 3 0.5g / L, NaCl0.5g / L, K 2 HPO 4 1.0g / L, MgSO 4 ·7H 2 O0.5g / L, FeSO 4 ·7H 2 O0.01g / L, agar 20g / L, pH value 7.2.

[0047] (2) Preparation of seed solution

[0048] Primary seed preparation: put 2.0cm on the above slope 2 The bacterial lawn was inserted into a 500mL Erlenmeyer flask containing 100mL of liquid seed medium, the shaking table was rotated at 160rpm, and cultured at a constant temperature of 30°C for 36h; secondary seed preparation: the primary seed liquid was inserted into 600mL of liquid seed medium In a 3.0L Erlenmeyer flask, the inoculum amount was 2% (v / v), the shaker rotated at 160rpm, ...

Embodiment 17

[0054] (1) Activation and preservation of strains

[0055] Streptomyces lilacinus ( Streptomyces lavendulae ) UN-8 was inoculated on a solid slant medium, cultured at a constant temperature of 28°C for 5 days, and stored at 4°C for a short period of time. The concentration components of the solid slant medium prepared with distilled water are: soluble starch 10g / L, KNO 3 0.2g / L, NaCl0.2g / L, K 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O0.2g / L, FeSO 4 ·7H 2O0.005g / L, agar 20g / L, pH value 7.0.

[0056] (2) Preparation of seed solution

[0057] Primary seed preparation: put 1.0cm on the above slope 2 The bacterial lawn was inserted into a 500mL Erlenmeyer flask containing 100mL of liquid seed medium, the shaking table was rotated at 200rpm, and cultured at a constant temperature of 28°C for 48h; secondary seed preparation: the primary seed liquid was inserted into 600mL of liquid seed medium In a 3.0L Erlenmeyer flask, the inoculum size was 5% (v / v), the shaker was rotated at 200 rp...

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Abstract

The invention discloses a method using streptomyces lavendulae fermentation to produce alpha-glucosidase inhibitors. The method is characterized in that strain activation, seed fermentation broth preparation and liquid fermentation are performed on the strains of Streptomyces lavendulae UN-8 with the preservation number being No. M2015512 to obtain fermentation broth rich in alpha-glucosidase inhibitors. The method has the advantages that the method can widely use cheap carbon and nitrogen sources such as soluble starch, cassava starch, corn starch, dried corn steep liquor powder and inorganic ammonium salt; the method adopting liquid-submerged fermentation is convenient to operate and implement, free of pollution, high in production efficiency and capable of satisfying the requirements of industrial production; preliminary identification shows that the alpha-glucosidase inhibitors produced by the method are novel alpha-glucosidase inhibitors, the alpha-glucosidase inhibitors can intensely inhibit alpha-glucosidase, and the IC50 of the alpha-glucosidase inhibitors is 321 times of that of the commercially available alpha-glucosidase inhibitor, namely acarbose; in-vivo experiments show that the alpha-glucosidase inhibitors can evidently improve the formation of postprandial hyperglycemia, the effect of the alpha-glucosidase inhibitors is equivalent to that of the acarbose, and the alpha-glucosidase inhibitors can be used for preventing and treating diabetes.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting and producing alpha-glucosidase inhibitors from Streptomyces lilacinus griseus. Background technique [0002] Diabetes mellitus is a comprehensive metabolic disease with persistent high blood sugar as the main biochemical feature. Clinically, it is mainly divided into type I diabetes and type II diabetes. In my country, type II diabetes is the majority. The clinical symptoms are abnormally high glucose concentration in blood and urine. Patients have obvious symptoms of polydipsia, polyphagia, polyuria, and weight loss, accompanied by symptoms of fatigue and weakness. Drugs for the treatment of type II diabetes mainly include insulin and its analogues, insulin secretagogues, insulin sensitizers, and α-glucosidase inhibitors. Among them, α-glucosidase inhibitors have been recommended as the first-line drug for lowering postprandial blood su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04C12R1/56
Inventor 曾伟梁智群陈桂光黄熙李娟
Owner GUANGXI UNIV
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