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Preparation method of feeding type microecological preparation and application of feeding type microecological preparation

A technology of micro-ecological preparations and bacterial preparations, which can be used in anti-toxins, pharmaceutical formulas, and medical preparations containing active ingredients, etc. It can solve the problems of antibiotic prohibition, relieve poisoning symptoms, promote rapid reproduction, and have a large amount of bacteria. Effect

Active Publication Date: 2016-02-03
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, due to safety concerns, antibiotics are gradually banned

Method used

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  • Preparation method of feeding type microecological preparation and application of feeding type microecological preparation
  • Preparation method of feeding type microecological preparation and application of feeding type microecological preparation
  • Preparation method of feeding type microecological preparation and application of feeding type microecological preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the preparation of feeding type probiotics

[0043] The feeding type microecological preparation provided in this example is composed of active ingredients and auxiliary materials; the active ingredients are composed of Lactobacillus casei, Candida tropicalis and Bacillus subtilis; the auxiliary materials are made of sodium alginate, calcium chloride and chitosan become.

[0044] The feeding-type microecological preparation provided in this example is prepared according to the following method: mix the Lactobacillus casei inoculum, Candida tropicalis and Bacillus subtilis inoculum to obtain the feeding-type probiotics; Lactobacillus casei inoculum The active ingredient of Lactobacillus casei ACCC10640; the active ingredient of Candida tropicalis ACCC21161; the active ingredient of Bacillus subtilis ACCC02157.

[0045] Lactobacillus casei inoculum is prepared by the following method: Lactobacillus casei-sodium alginate liquid is added dropwise to calcium c...

Embodiment 2

[0062] Embodiment 2, feeding type probiotics is used for the treatment of white goat ruminal acidosis

[0063] Healthy male Anhui white goats aged about 4 months and weighing about 20kg were selected as experimental animals. They were modeled by over-feeding grains and fed with cornmeal at a rate of 40g / kg. Rumen fluid examination, blood test, and feces were checked before and after modeling. Urinary pH and ciliate counts. Select 20 Anhui white goats with successful modeling and randomly divide them into a control group and a feeding-type microecological preparation group (hereinafter referred to as the experimental group). The control group used rehydration [intravenous infusion of normal saline or 5% (mass percentage) glucose water] to increase blood volume, promote blood circulation, and relieve acidosis symptoms; Feed-type probiotic preparations were gavaged, and the feed-type probiotic preparations of Example 1 were suspended in distilled water to obtain feed-type probio...

Embodiment 3

[0108] The optimization of the preparation conditions of the feeding type probiotics of embodiment 3, embodiment 1

[0109] Investigation of microsphere characteristics: When the microspheres of each strain were prepared, they were placed at room temperature for 1 month after preparation, and the bacterial load was measured 2 months later. Weigh 1g of microspheres, stir in sterilized distilled water, filter with filter paper, take the supernatant to measure the number of viable bacteria, which is the surface bacteria content. Take an equal amount of microspheres, crush them in sterilized distilled water, shake at a constant temperature of 37°C for 24 hours, filter with filter paper, take the supernatant to measure the number of viable bacteria, which is the total bacterial count, and the bacterial load = total bacterial count - surface bacteria count.

[0110] 1. The influence of single factor investigation on the bacterial load of microspheres of each strain

[0111] Lactob...

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Abstract

The invention discloses a preparation method of a feeding type microecological preparation and application of the feeding type microecological preparation. The feeding type microecological preparation is prepared by mixing a lactobacillus casei inoculant, a candida tropicalis inoculant and a bacillus subtilis inoculant. An active ingredient of the lactobacillus casei inoculant is lactobacillus casei; an active ingredient of the candida tropicalis inoculant is candida tropicalis; an active ingredient of the bacillus subtilis inoculant is bacillus subtilis. The feeding type microecological preparation is high in fungal burdening capacity, stable in structure and capable of promoting rapid propagation of infusorians after rumen acidosis of goats to enable continuous increase of volatile fatty acids in rumen fluid of the goats, a recovery process is shortened while cellulose fodder digestive ability of the goats is improved, and rumen acidosis symptoms of the goats can be effectively alleviated. The feeding type microecological preparation prepared according to the preparation method is capable of quickly rebuilding ruminal environments of the goats to recover physiological functions and is significant to animal husbandry production.

Description

technical field [0001] The invention relates to a preparation method of a feeding type microecological preparation and an application of the feeding type microecological preparation. Background technique [0002] In recent years, with the development of large-scale aquaculture, the proportion of concentrate in ruminant diets has increased significantly. Although concentrate can significantly improve animal growth performance, long-term large-scale feeding of concentrate will cause rumen acidosis. Rumen acidosis can cause the dehydration reaction of the stomach and intestinal epithelium. In severe cases, it can cause the pH value of the blood to decrease, and the body will suffer from dehydration and other serious consequences. Data show that the elimination rate of cows suffering from subacute acidosis can often be higher than 45% every year, and severe acute acidosis can even cause death, and the mortality rate can be as high as 90%. One of the main prevention and treatmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/064A61P39/02A61K35/747A61K35/742
Inventor 韩春杨何燕飞顾金刚李世贵赵秀峰刘翠艳龚明波张瑞颖马晓彤张晓霞王宇洲
Owner ANHUI AGRICULTURAL UNIVERSITY