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Preparation used for controlling vibrio number in water, and preparation method and application method thereof

A vibrio, quantitative technology, applied in the fields of botanical equipment and methods, chemicals used for biological control, applications, etc., can solve the problems of secondary pollution of water bodies, and achieve the problem of not easy secondary pollution and resistant to drug resistance. Effect

Inactive Publication Date: 2016-02-10
SHANGHAI OCEAN UNIV +1
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is a preparation for controlling the number of Vibrio in water bodies. The use of the bactericide will not cause the problem of drug resistance of Vibrio, and avoid secondary pollution of water bodies caused by the use of chemical drugs

Method used

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  • Preparation used for controlling vibrio number in water, and preparation method and application method thereof

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Embodiment Construction

[0011] 1. Experimental materials

[0012] 1. High-concentration bacteriophage Bdellovibrio raw powder, the concentration is greater than 300 million per gram (the counting method of high-concentration Bdellovibrio raw powder is recorded in the Chinese patent literature with the patent number ZL201110347334.2), purchased in Shanxi Biological Veterinary Medicine Feed Engineering Technology Research Center.

[0013] 2. Bacillus amyloliquefaciens microcapsules, the concentration of which is greater than 2 billion per gram (Bacillus amyloliquefaciens microcapsules and its preparation method are recorded in Chinese patent literature with patent application number 201210297575.5), prepared and supplied by Shanghai Ocean University.

[0014] 3. Soluble starch, with an effective content greater than 99%, was purchased from Sinopharm Chemical Reagent Co., Ltd.

[0015] 4. Povidone-iodine preparation for aquatic products, with an effective content of 10%, produced by Wuxi Huanuowei Anim...

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Abstract

The invention discloses a preparation used for controlling vibrio number in water, and a preparation method and an application method thereof. The bactericide is composed of high-concentration bdellovibrio bacteriovorus original powder with a concentration higher than 3.3billion / g, bacillus amyloliquefaciens microcapsule with a concentration higher than 2billion / g, and soluble starch with an effective content higher than 99%. A mass ratio of the high-concentration bdellovibrio bacteriovorus original powder to the bacillus amyloliquefaciens microcapsule to the soluble starch is 4:2:3. According to the preparation used for controlling vibrio number in water, the main components are bdellovibrio bacteriovorus and bacillus amyloliquefaciens. The action mechanism is that vibrio can be cracked and inhibited fast. Compared to chemical agents such as povidone-iodine which is used for killing bacteria through releasing free iodine and breaking bacterial cell metabolism, the preparation is safer and more environment-friendly. With the preparation, secondary pollution is prevented, and vibrio drug resistance is prevented.

Description

technical field [0001] The invention belongs to the technical field of water treatment, and relates to a preparation for controlling the quantity of Vibrio in water, and a preparation and use method of the preparation. Background technique [0002] Vibrio is the most common pathogen in aquaculture. Aquaculture vibriosis caused by Vibrio occurs widely all over the world. It has a high incidence and great harm, causing huge economic losses to aquaculture. In recent years, with the widespread use of a large number of antibiotics (such as povidone-iodine preparations for aquatic products), the problem of Vibrio cross-resistance and multi-drug resistance has become increasingly serious, and conventional drugs have been difficult to control the number of Vibrio in aquaculture water. Therefore, it is imminent to seek new methods to control the number of Vibrio in aquaculture water. [0003] In addition, the pollution problem caused by Vibrio also exists in the landscape water body...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P1/00C02F1/50
Inventor 曹海鹏李家胜何珊
Owner SHANGHAI OCEAN UNIV
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