Bacterial strain and microbial agent for preventing and curing apple tree rotten diseases and preparation method of microbial agent

A technology for apple tree rot disease and microbial inoculum, which is applied in the field of strains for preventing and controlling apple tree rot disease, can solve the problems of difficulty in wide application, difficulty in long-term colonization, complicated extraction process, etc., and achieves the effect of no toxic and side effects

Inactive Publication Date: 2016-02-17
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, Duan Zemin, Wang Xianping, Zhou Boling, etc. Research on pollution-free control technology of apple tree rot [J], Shanxi Agricultural Sciences, 2002, 30 (4): 55-59 discloses the use of multi-flavored traditional Chinese medicines such as capillary, flavescens and skullcap. It is used for the prevention and treatment of rot disease, but the composition of traditional Chinese medicine is complex, the extraction process is cumbersome, and the production cost is high, so it is difficult to be widely used
Jiao Rongbin. A new drug for the prevention and treatment of a

Method used

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  • Bacterial strain and microbial agent for preventing and curing apple tree rotten diseases and preparation method of microbial agent
  • Bacterial strain and microbial agent for preventing and curing apple tree rotten diseases and preparation method of microbial agent
  • Bacterial strain and microbial agent for preventing and curing apple tree rotten diseases and preparation method of microbial agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Isolation, purification and identification of Chaetomium globosa for preventing and controlling apple tree rot

[0026] 1. Separation and purification

[0027] It is isolated from the bark of apple trees in the apple orchard of Baishui County, Shaanxi Province. Take 5g of dried apple tree bark, cut it into 1cm pieces, put it into a 1000ml triangular flask, add 10ml of special disinfectant, and shake it for 6-8min. Then add 600ml of sterile water, absorb 2ml of this suspension and spread it on the Martin medium plane, pour off the water, use tweezers to remove the warped skin and put it in a sterile petri dish, cut into small pieces and put them on the Martin medium plane, Incubate at 28°C.

[0028] After the clearly identifiable Chaetomium ascomycetes grew on the separation medium, a single ascomyce was picked under a dissecting microscope, and inoculated in water agar medium or PDA medium for culture and purification.

[0029] 2. Separation and identifica...

Embodiment 2

[0035] Embodiment 2: the control effect determination of the Chaetomium globosa of controlling apple tree rot

[0036] The control effect of 61239 Chaetomium globosa on apple tree rot was determined by plate confrontation culture method and mycelial growth rate method indoors. The confrontation culture results show that it has obvious inhibitory effect on the mycelium growth of apple tree rot fungus, see figure 1 . After confrontation culture for 8 days, a transparent antibacterial zone with a diameter of 13 mm was formed around the biocontrol bacteria. It can be seen under a microscope that the mycelium of the pathogenic bacteria around the antibacterial zone grows slowly, the top of the mycelia expands, and the cell wall is destroyed, and the protoplasts leak out. exhibits bacteriolysis, see figure 1 .

[0037] The 61239 Chaetomium globosa strain was selected for liquid fermentation, and the results showed that its fermentation filtrate had a significant inhibitory effect...

Embodiment 3

[0041] Embodiment 3: the preparation of the microbial inoculant of preventing and treating apple tree rot

[0042] (1) First-level seed cultivation: connect the slant spores of the chaetomium globosa strain 61239 according to claim 1 into the sterilized first-level seed culture medium, at a culture temperature of 32-36°C, with an air flow of 1:0.4-0.8 Cultivate for 45-60 hours under the condition of v / v / min and stirring; among them, the acquisition of primary seed medium: add 50g barley and 40g water to a 500ml Erlenmeyer flask, the pH is natural, stir evenly, and then sterilize at 121°C for 40min.

[0043] (2) Secondary seed cultivation: Inoculate 5-15% of the inoculum into the sterilized secondary seed culture medium, at a cultivation temperature of 32-36°C, with an aeration rate of 1: 0.8-1.2v / v / min and Cultivate under stirring conditions for 20-30 hours to obtain secondary seeds; among them, the acquisition of secondary seed medium: weigh 5Kg barley and 4Kg water, mix well...

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Abstract

Provided are a bacterial strain and a microbial agent for preventing and curing apple tree rotten diseases and a preparation method of the microbial agent. The strain 61239 is preserved in China Center for Type Culture Collection which is called CCTCC for short on July 1st, 2015, and the preservation number is CCTCC M2015420. The invention further comprises the microbial agent containing chaetomium globosum for preventing and curing the apple tree rotten diseases and the preparation method of the microbial agent. Biological control fungi come from the natural world, pollution, public hazards and residues do not exist, toxic and side effects on people and livestock do not exist, the safety is achieved for apple trees, human bodies and ecological environments, the biological control fungi can be applied to any growth period of the apple trees, specially a dormant period, no resistance is produced, and the apple rotten diseases can be thoroughly controlled and radically cured. It is proved through experiments that specificity of the chaetomium globosum strain 61239 to apple tree rotten germs is high, the control effect is good, and the average control effect is 95 percent or above.

Description

technical field [0001] The invention relates to a bacterial strain, a microbial agent and a preparation method thereof, in particular to a bacterial strain for preventing and treating apple tree rot, a microbial agent and a preparation method thereof. Background technique [0002] Apple tree rot (Valsa cankerofapple) is a regional disease, mainly occurs in my country, Japan and Korea, and sporadically distributed in the United States, Canada, Britain and Russia. In history, there have been five rot disease pandemics in my country, which have caused devastating blows to the apple industry in various periods. Since the 1950s, the use of protective chemical fungicides to spray the whole garden to prevent the disease, as well as surgical scaling and smearing of systemic therapeutic fungicides on the diseased parts of apple trees have failed. achieve the desired effect. [0003] In recent years, the prevention and treatment of apple rot disease still uses chemical agents as the...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
Inventor 郭云忠朱明旗孙广宇
Owner NORTHWEST A & F UNIV
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