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Method for two-aqueous-phase extraction of nevadensin

A lycocylin and two-phase water-phase technology, which is applied in the field of separation and purification of active ingredients of natural products, achieves the effects of high efficiency, rapid extraction, and easy scale-up production

Active Publication Date: 2016-02-24
SHENZHEN XIANHU BOTANICAL GARDEN ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Licotyrandin has the effect of inhibiting tuberculosis in vitro. Clinically, it has been initially used to treat patients with cervical lymphatic tuberculosis. No obvious side effects have been found, and it is still in trial.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Pulverizing the raw material of the hanging stone lettuce to 20 meshes to obtain the raw material powder of the hanging stone lettuce, which is sealed and stored. Weigh 10g of the raw material powder of the hanging stone lettuce and add it into the two-phase system, the ratio of solid to liquid is 1g / 18ml. The extraction was carried out for 80 minutes under stirring and shaking, and the extraction temperature was 45°C. Take the lycocylin-enriched phase (sodium carbonate solution phase), extract it three times with solvent chloroform, collect the chloroform extract and carry out vacuum distillation to dryness, the temperature of vacuum distillation is 40 ° C, the concentrate is dissolved in ethanol, and the second decompression Distilled to dryness, the temperature of vacuum distillation was 45° C., and the extract lycophyllin was obtained, the content (purity) of which was 97.10% by mass fraction.

[0020] Described two-phase system, it contains solute ethanol and sodi...

Embodiment 2

[0022] Pulverizing the raw material of the hanging stone lettuce to 20 meshes to obtain the raw material powder of the hanging stone lettuce, which is sealed and stored. Weigh 10g of the raw material powder of the hanging stone lettuce and add it into the two-phase system, the ratio of solid to liquid is 1g / 30ml. The extraction was carried out for 80 minutes under stirring and shaking, and the extraction temperature was 45°C. Take the lycocylin-enriched phase (sodium carbonate solution phase), extract it three times with solvent chloroform, collect the chloroform extract and carry out vacuum distillation to dryness, the temperature of vacuum distillation is 40 ° C, the concentrate is dissolved in ethanol, and the second decompression After distillation and drying, the temperature of vacuum distillation is 45° C. to obtain the extract lycophyllin, the content (purity) of which is 89.34% by mass fraction.

[0023] Described two-phase aqueous system, it contains solute ethanol a...

Embodiment 3

[0025] Pulverizing the raw material of the hanging stone lettuce to 20 meshes to obtain the raw material powder of the hanging stone lettuce, which is sealed and stored. Weigh 10g of the raw material powder of the hanging stone lettuce and add it into the two-phase system, the ratio of solid to liquid is 1g / 15ml. The extraction was carried out for 80 minutes under stirring and shaking, and the extraction temperature was 45°C. Take the lycocylin-enriched phase (sodium carbonate solution phase), extract it three times with solvent chloroform, collect the chloroform extract and carry out vacuum distillation to dryness, the temperature of vacuum distillation is 40 ° C, the concentrate is dissolved in ethanol, and the second decompression After distillation and drying, the temperature of vacuum distillation is 45° C. to obtain the extract lycophyllin, the content (purity) of which is 94.74% by mass fraction.

[0026] Described two-phase system, it contains solute ethanol and sodiu...

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Abstract

The invention discloses a method for two-aqueous-phase extraction of nevadensin. Lysionotus pauciflorus raw material powder is added to a two-aqueous-phase system for extraction, and a nevadensin enriched phase is transferred and taken after extraction is completed, nevadensin in the nevadensin enriched phase is extracted through an organic solvent, extract is concentrated and dried, and nevadensin extract is obtained. Water serves as the solvent of the two-aqueous-phase system, ethyl alcohol and inorganic salt serve as solutes of the two-aqueous-phase system, the mass fraction of ethyl alcohol ranges from 10% to 30%, the mass fraction of inorganic salt ranges from 15% to 20%, and inorganic salt is ammonium sulfate or dipotassium phosphate or sodium carbonate or sodium bicarbonate. The method for two-aqueous-phase extraction of nevadensin has the advantages that moderate production conditions are achieved, the technology is easy to amplify, production is rapid, the solvent is easily recycled, environment friendliness and free pollution are achieved, and the quality of nevadensin is high.

Description

Technical field: [0001] The invention belongs to the field of separation and purification of effective components of natural products, and in particular relates to a method for extracting shicrophyllin with two aqueous phases. Background technique: [0002] Lysionotus pauciflorus Maxim, a plant of the Gesneriaceae family, is widely distributed in southern my country, and Guizhou is particularly rich in resources. It is a commonly used medicinal material of the Miao nationality. The whole plant is used as medicine. Analgesic effect. It is clinically used to treat lymphatic tuberculosis, chronic bronchitis, hematemesis, bruises, cough due to lung heat, metrorrhagia, bacillary dysentery, malnutrition, and rheumatic arthralgia. This herbal medicine is used in the treatment of lymphatic tuberculosis among the Miao people. It has remarkable curative effect in clinical application and is non-toxic and has no side effects. Gesneriaceae plants Loxostigmaca avalerie (Level. et Van.),...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/30C07D311/40
Inventor 邱志敬陈朋谢锐星彭杨杨平
Owner SHENZHEN XIANHU BOTANICAL GARDEN ADMINISTRATION