A kind of oleanolic acid molecularly imprinted polymer and its preparation method and application
A technology of oleanolic acid and molecular imprinting, applied in chemical instruments and methods, other chemical processes, etc., can solve the problems of low preparation efficiency, poor pertinence and predictability, large workload, etc., achieve good adsorption performance, overcome the shape Irregular, fast binding effects
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Embodiment 1
[0038] (1) Simulation of molecularly imprinted polymer pre-assembly system
[0039] (a) Using Gaussian software, use the density functional method b3lyp to optimize the gas phase geometry of template molecules and functional monomers at the 6-31G(d) level, and determine that each optimized structure is a minimum point, which is useful for the calculation Frequency analysis of the molecular structure of the molecular structure, all molecules have no imaginary frequency, the optimized structure of the three molecules is as follows figure 1 shown.
[0040] (b) At the same level, calculate the NBO charge distribution of the template and the monomer molecule, and analyze the possible binding sites of the template and the functional monomer. The charge distribution of the three molecules is shown in figure 2 . OA contains a carboxyl group and a hydroxyl group. Two H atoms and three O atoms in the two groups can act as proton donors and acceptors to form hydrogen bonds with functi...
Embodiment 2
[0064] (1) The simulation of the molecularly imprinted polymer pre-assembly system is the same as in Example 1.
[0065] (2) The preparation of monodisperse Poly(MAA-co-DVB) polymeric microspheres is the same as in Example 1.
[0066] (3) Dissolve 0.08 mmol OA in 2.0 mL ethanol, then add 6.0 mL acetonitrile and 0.4 mmol MAA, after the solution is mixed, add 2.0 mmol mixed cross-linking agent DVB / EGDMA (mol / mol, 4:1) and initiate AIBN 6.44 mg, sonicated for 2 min, and kept in the dark for 4 h to obtain a pre-polymerization solution; 200 mg of the polymer microspheres obtained in step (2) was added to the pre-polymerization solution, mixed well, and nitrogen gas was passed for 10 min, sealed, and placed in Polymerize at 65°C for 24 h to obtain OA-MIPs polymer; filter the obtained polymer through a G4 sand core funnel, and rinse with acetonitrile, acetone and methanol respectively. The obtained polymer was added to 100 mL methanol / acetic acid mixed solution (8:2, V / V), and Soxhl...
Embodiment 3
[0070] (1) The simulation of the molecularly imprinted polymer pre-assembly system is the same as in Example 1.
[0071] (2) Preparation of monodisperse Poly(MAA-co-DVB / EGDMA) polymeric microspheres
[0072] Dissolve 0.4 mmol of functional monomer MAA, 2.0 mmol of mixed cross-linking agent DVB / EGDMA (mol / mol, 4:1) and 6.44 mg of initiator AIBN in 8 mL of acetonitrile. Polymerized at 65°C for 24 h to obtain polymer microspheres; the obtained polymer was suction-filtered through a G4 sand core funnel, washed with tetrahydrofuran, acetone and ether respectively, and dried for later use.
[0073] (3) Dissolve 0.1 mmol OA in 2.0 mL ethanol, then add 6.0 mL acetonitrile and 0.4 mmol MAA, after the solution is mixed, add 2.0 mmol mixed cross-linking agent DVB / EGDMA (mol / mol, 4:1) and initiate AIBN 6.44 mg, sonicated for 2 min, and kept in the dark for 4 h to obtain a pre-polymerization solution; 200 mg of the polymer microspheres obtained in step (2) was added to the pre-polymerizat...
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