Preparation method of coumarin molecularly-imprinted electrochemical sensor

A molecular imprinting and coumarin technology, applied in the direction of electrochemical variables of materials, can solve the problems of poor regeneration and reversibility, high detection limit, slow electron transfer speed and response, and achieve high affinity and selectivity. improved effect

Inactive Publication Date: 2016-02-24
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the thickness of the imprinted film prepared by the traditional imprinting method is difficult to control, and the high cross-linking degree makes the electron transfer speed and response slow, the detection limit is high, and the regeneration and reversibility are poor, which affects the application of molecular imprinting technology in electrochemical sensors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Pretreatment of glassy carbon electrodes: use 0.5 μm, 0.05 μm Al 2 o 3 The surface of the powder was polished, then ultrasonically cleaned with twice distilled water, washed several times with absolute ethanol, dried with nitrogen, and soaked in γ-mercaptopropyltriethoxysilane containing 25% by mass In the dehydrated ethanol solution, soak at room temperature for 5 hours, after taking it out, wash it several times with dehydrated ethanol, dry, and obtain the pretreated glassy carbon electrode;

[0019] (2) Preparation of nano-gold modified glassy carbon electrode: Put the pretreated glassy carbon electrode into 0.12mol / L chloroauric acid solution, soak it at room temperature for 11h, wash it with deionized water after taking it out, and then put it in the mass percent concentration 20% hydrazine hydrate solution for 2 hours, washed with deionized water after taking it out, and dried naturally to obtain nano-gold modified glassy carbon electrode;

[0020] (3) Prepa...

Embodiment 2

[0023] (1) Pretreatment of glassy carbon electrodes: use 0.5 μm, 0.05 μm Al 2 o 3 The surface of the powder was polished, then ultrasonically cleaned with twice distilled water, washed several times with absolute ethanol, dried with nitrogen, and soaked in γ-mercaptopropyltriethoxysilane containing 20% ​​by mass soaked at room temperature for 6 hours, washed several times with absolute ethanol, and dried to obtain a pretreated glassy carbon electrode;

[0024] (2) Preparation of nano-gold modified glassy carbon electrode: Put the pretreated glassy carbon electrode into 0.12mol / L chloroauric acid solution, soak it at room temperature for 10h, wash it with deionized water after taking it out, and then put it in the mass percent concentration 20% hydrazine hydrate solution for 2 hours, washed with deionized water after taking it out, and dried naturally to obtain nano-gold modified glassy carbon electrode;

[0025] (3) Preparation of coumarin molecularly imprinted polymer: In t...

Embodiment 3

[0028] (1) Pretreatment of glassy carbon electrodes: use 0.5 μm, 0.05 μm Al 2 o 3 The powder was surface polished, then ultrasonically cleaned with twice distilled water, washed several times with absolute ethanol, dried with nitrogen, and soaked in γ-mercaptopropyltriethoxysilane containing 00% by mass In the dehydrated ethanol solution, soak at room temperature for 4 hours, after taking it out, wash it several times with dehydrated ethanol, dry, and obtain the pretreated glassy carbon electrode;

[0029] (2) Preparation of nano-gold modified glassy carbon electrode: Put the pretreated glassy carbon electrode into 0.12mol / L chloroauric acid solution, soak it at room temperature for 12h, wash it with deionized water after taking it out, and then put it in the concentration of mass percent 20% hydrazine hydrate solution for 2 hours, washed with deionized water after taking it out, and dried naturally to obtain nano-gold modified glassy carbon electrode;

[0030] (3) Preparati...

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PUM

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Abstract

The invention discloses a preparation method of a coumarin molecularly-imprinted electrochemical sensor. The preparation method is characterized by comprising the steps of firstly, modifying a glassy carbon electrode with a silane coupling agent and nano-gold, adding, by mass, 12-22% of ethylene glycol dimethacrylate, 2.5-10% of methyl methacrylate, 63-83% of N, N-dimethylformamide, 1.0-2.0% of dimethyl 2,2'-azobis(2-methylpropionate) and 1.0-4.0% of coumarin into a reactor, stirring and dissolving the ingredients, introducing nitrogen for conducting deoxygenation for 10 minutes, conducting stirring and a reaction for 10-12 hours at the temperature of 75-80 DEG C under the nitrogen atmosphere, removing template molecules with a mixed solution of ethyl alcohol and hydrochloric acid, conducting drying, obtaining coumarin molecularly-imprinted polymer, then applying the polymer to a modified electrode, obtaining the coumarin molecularly imprinted electrochemical sensor, and connecting the sensor with an electrochemical workstation for forming a sensor capable of conducting specific template molecular recognition. The sensor prepared through the preparation method is low in cost, high in sensitivity, good in specificity, high in detection speed and capable of being used repeatedly.

Description

technical field [0001] The present invention relates to a preparation method of molecularly imprinted sensor and the technical field of rapid detection application, in particular to a preparation method of coumarin molecularly imprinted electrochemical sensor, which is based on molecularly imprinted specific recognition and is used to detect drugs , food, and coumarin technology in biological samples. Background technique [0002] Coumarin (coumarin) is widely found in vanilla plants such as natural tonka bean, snakewort, wild vanilla and orchid, and can be used to make fragrances and act as a fixative. Coumarins are similar in structure to vitamin K and are a class of oral anticoagulant drugs. Dicoumarins can also be used to deal with rodents. Coumarin compounds have a variety of derivatives, and different derivatives have different medicinal value. The core structure of this type of compound has three types: simple coumarins, furanocoumarins, and pyranocoumarins. It is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 李慧芝许崇娟张为民
Owner UNIV OF JINAN
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