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A kind of micro-volume ultraviolet spectrophotometer quality detection method and detection kit

A spectrophotometer and kit technology, used in chemical analysis and biological fields, can solve the problems of no detection pool, only detection window, and can not be placed in a cuvette, etc., and achieves low uncertainty, wide detection range, and operation. easy effect

Active Publication Date: 2018-02-13
NAT INST OF METROLOGY CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the micro-volume ultraviolet spectrophotometer is highly integrated, has only a detection window, no detection cell, cannot be placed in a cuvette, and the wavelength cannot be adjusted. Therefore, the detection indicators specified in JJGl78-2007 can hardly be completed, and various technical indicators of its production It is also significantly different from the provisions of JJGl78-2007, so JJGl78-2007 is not suitable for the detection and calibration of micro-ultraviolet spectrophotometers

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  • A kind of micro-volume ultraviolet spectrophotometer quality detection method and detection kit
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  • A kind of micro-volume ultraviolet spectrophotometer quality detection method and detection kit

Examples

Experimental program
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Effect test

Embodiment 1

[0072] Example 1 Relation between salmon sperm DNA and ultraviolet absorption signal

[0073] 1. Purpose of the experiment:

[0074] To study whether there is a linear relationship between the concentration of salmon sperm DNA and the ultraviolet absorption signal, to prove that the micro-volume ultraviolet spectrophotometer can be used to quantify salmon sperm DNA, and to prove that the micro-volume UV spectrophotometer can be used for quality inspection by using salmon sperm DNA standard substances.

[0075] 2. Instruments and equipment:

[0076] Micro-volume UV spectrophotometer Nanodrop2000 (Thermo, USA); pipette 0.5 μL-10 μL (eppendorf Finland)

[0077] 3. Materials and methods:

[0078] 1. The blank control sample is 18.2mΩ.cm ultrapure water, and its salmon sperm DNA concentration is 0ng / μL.

[0079] 2. Salmon sperm DNA standard substances, concentrations are: 10.7ng / μL, 103.9ng / μL, 508.1ng / μL, 1048ng / μL, 2163ng / μL. The dissolving solvent of salmon sperm DNA standar...

Embodiment 2

[0097] Example 2 Determine the linear range, limit of quantification and detection sensitivity of the micro-volume ultraviolet spectrophotometer to measure the DNA content of salmon sperm

[0098] 1. Purpose of the experiment:

[0099] To study the linear range, limit of quantification and detection sensitivity of micro-volume ultraviolet spectrophotometer to measure salmon sperm DNA content, so as to prove that micro-volume ultraviolet spectrophotometer can be used to quantify salmon sperm DNA.

[0100] 2. Instruments and equipment:

[0101] Trace ultraviolet spectrophotometer 1Nanodrop2000 (Thermo, the United States);

[0102] Micro-volume UV spectrometer 2Gene Quant (Amersham Biosciences, USA);

[0103] Trace ultraviolet photometer 3Biophotometer (Eppendorf Finland),

[0104] Micro-volume UV spectrophotometer 4Nano Q (Boao Biological Co., Ltd. China);

[0105] Pipette 0.5 μL-10 μL (eppendorf Finland)

[0106] 3. Materials and methods

[0107] 1. Blank sample (0ng / μL) ...

Embodiment 3

[0126] Example 3 Inductively Coupled Plasma Emission Spectrophotometry Determination of Salmon Sperm DNA Mass Concentration

[0127] 1. Purpose of the experiment:

[0128] The study uses inductively coupled plasma spectroscopy to measure the content of salmon sperm DNA standard substance, so as to determine its characteristic value, and prove the reliability and traceability of the characteristic value of salmon sperm DNA standard substance.

[0129] 2. Instruments and equipment

[0130] Inductively coupled plasma emission spectrometer: Thermo iCAP6000, Thermo Company (USA); ultrapure water system: Millipore QS type, Millipore Company (USA); analytical balance: 0.0001g, 0.00001g Mettler (Switzerland).

[0131] 3. Materials and methods:

[0132] 1. Salmon sperm DNA standard substances, concentrations are: 10.7ng / μL, 103.9ng / μL, 508.1ng / μL, 1048ng / μL, 2163ng / μL; national primary standard substance of ammonium dihydrogen phosphate (GBW06502); electronic grade Nitric acid: 69%-...

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Abstract

The invention provides a micro-volume ultraviolet spectrophotometer quality detection method and a detection kit. The invention adopts a series of salmon sperm DNA standard substances for the first time to carry out quality inspection on a trace ultraviolet spectrophotometer. Its advantages are high sensitivity, which can detect DNA as low as 10.7ng; wide linear range: it can detect DNA from 0ng / μL to 2163ng / μL, spanning 4 orders of magnitude; covering the measurement range of the existing trace UV spectrophotometer on the market ; The standard substance of salmon sperm DNA is verified by the national standard device of ultraviolet spectrophotometer, and the accuracy is confirmed to be high. The invention solves the problem that the micro-ultraviolet spectrophotometer cannot be calibrated according to the existing national standards for spectrophotometer verification due to limitations such as only a detection window, inability to be placed in a cuvette, and non-adjustable wavelength.

Description

Technical field: [0001] The invention belongs to the field of biological and chemical analysis, and in particular relates to a quality detection method and a detection kit of a trace ultraviolet spectrophotometer. Background technique: [0002] Micro-volume UV spectrophotometer is a kind of UV spectrophotometer with small sample volume and small volume. It is very different from large-scale UV spectrophotometer. At present, there is no suitable reagent for quality detection of micro-volume UV spectrophotometer. Box and supporting method. [0003] The micro-volume ultraviolet spectrophotometer is characterized by high integration and high sensitivity, and is often used for nucleic acid, protein quantification and bacterial growth concentration quantification. Mainly distributed in inspection and quarantine laboratories, clinical testing laboratories, blood product centers, etc. In view of the fact that each laboratory gradually plays its responsibilities and roles as an ind...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33
Inventor 高运华盛灵慧隋志伟武利庆董莲华崔伟群
Owner NAT INST OF METROLOGY CHINA