Clerodane type diterpene compound for treating neuroglioma
A glioma and compound technology, applied in organic chemistry, antineoplastic drugs, drug combination, etc., can solve the problems of separation and identification of unseen flavonoid components
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Embodiment 1
[0025] Example 1: Compound (I) Separation Preparation and Structure Confirmation
[0026] Sources of medicinal materials and reagents: ethanol, petroleum ether, ethyl acetate, n-butanol, and dichloromethane are analytically pure, purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. Methanol, analytically pure, are purchased from Jiangsu Hanbang Chemical Reagent Co., Ltd.
[0027] Preparation method: (a) crush the dried near-ripe fruit (8kg) of cardamom, extract with 75% ethanol under heat reflux (25L×3 times), combine the extracts, concentrate until no alcohol smell (6L), and successively add petroleum ether (6L×3 times), ethyl acetate (6L×3 times) and water-saturated n-butanol (6L×3 times) were extracted and concentrated to obtain petroleum ether extract, ethyl acetate extract (315g) and n-butanol Alcohol extract; (b) ethyl acetate extract in step (a) is dissolved to 2L with purified water, and medical absorbent cotton is filtered, and filtrate is separated with AB-8 t...
Embodiment 2
[0029] Embodiment 2: compound (I) pharmacological action test
[0030] 1. Materials and Instruments
[0031] In this experiment, the human brain malignant glioblastoma U251 cell line was purchased from the American Type Culture Collection. DMEM high-glucose medium was purchased from Hyclone Company of the United States; sodium chloride, sodium hydroxide, potassium chloride, oxygen chloride, sodium hydroxide, disodium hydrogen phosphate, sodium dihydrogen phosphate, potassium dihydrogen phosphate, and methanol were purchased from Nanjing chemical plant. Compound (I) is self-made, and the HPLC normalized purity is greater than 98%. Glycine, Tris, Tween20, and dimethyl sulfoxide (DMEM) were purchased from Sigma, USA; Aimexin-V / PI cell death kit was purchased from Invitrogen, USA; cell lysate, 0.25% trypsin, CellCountingKit-8 kit , penicillin, streptomycin, Hoechst33258, nuclear dye DAPI, PMSF, Westernblot gel preparation kit, nuclear protein extraction kit, Coomassie brilliant...
Embodiment 3
[0056] Preparation of tablets: firstly prepare compound (I) according to the method of Example 1, and utilize organic acids such as tartaric acid, or citric acid, formic acid or oxalic acid, etc., inorganic acids such as hydrochloric acid or sulfuric acid or phosphoric acid to make salts, according to its The weight ratio of the excipient to the excipient is 1:9, and the excipient is granulated and compressed into tablets.
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