Preparation method for special low-temperature amylase for livestock feedstuff

A low-temperature amylase, livestock and poultry feed technology, applied in the direction of biochemical equipment and methods, animal feed, enzymes, etc., can solve the problems of low digestion utilization rate and high effective temperature of amylase, so as to promote digestion and absorption, and improve animal growth rate. and uniformity, reducing the effect of nutritional diarrhea

Inactive Publication Date: 2016-03-23
HANGZHOU BIOCOM BIOLOGICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The invention provides a preparation method of low-temperature amylase special for livestock and poultry feed. The low-temperature amylase solves the problem of high effective temperature of amylase in the prior art, and is applied to compound feed for livestock and poultry such as pigs and chickens to improve the efficiency of livestock and poultry. The digestion and utilization rate of starch by poultry can solve the problem of low digestion and utilization rate of starch by livestock and poultry, and save feed resources

Method used

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  • Preparation method for special low-temperature amylase for livestock feedstuff
  • Preparation method for special low-temperature amylase for livestock feedstuff

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Embodiment 1

[0029] A kind of preparation method of low-temperature amylase special for livestock and poultry feed of the present embodiment, comprises the following steps:

[0030]1) Primary slant culture: inoculate the low-temperature amylase-producing bacteria on the slant medium, and culture it at 25°C to 35°C for 4-8 days to obtain the first-grade slant seed spores; the slant medium is a mixture prepared according to the following ratio Solution: sodium nitrate 3.0~5.0g / L, dipotassium hydrogen phosphate 0.5~1.5g / L, magnesium sulfate 0.5~5g / L, potassium chloride 1.0~10g / L, ferrous sulfate 1~5g / L, sucrose 10 ~30g / L, agar 15g;

[0031] 2) Secondary cultivation: inoculate the spores of the primary slant seeds in step 1) into the liquid culture medium, the liquid filling volume is 50-100mL / 500mL, and cultivate at 25°C-35°C, 160-300rpm / min for 48- 55h to obtain the secondary seed liquid; the liquid medium is a mixed liquid prepared according to the following ratio: corn starch 5-40g / L, soy...

Embodiment 2

[0035] A kind of preparation method of low-temperature amylase special for livestock and poultry feed of the present embodiment, comprises the following steps:

[0036] 1) Primary slant culture: inoculate the low-temperature amylase-producing bacteria on the slant medium, and culture it at 25°C to 35°C for 4-8 days to obtain the first-grade slant seed spores; the slant medium is a mixture prepared according to the following ratio Solution: sodium nitrate 3.0~5.0g / L, dipotassium hydrogen phosphate 0.5~1.5g / L, magnesium sulfate 0.5~5g / L, potassium chloride 1.0~10g / L, ferrous sulfate 1~5g / L, sucrose 10 ~30g / L, agar 15g;

[0037] 2) Secondary cultivation: inoculate the spores of the primary slant seeds in step 1) into the liquid culture medium, the liquid filling volume is 50-100mL / 500mL, and cultivate at 25°C-35°C, 160-300rpm / min for 48- 55h to obtain the secondary seed liquid; the liquid medium is a mixed liquid prepared according to the following ratio: corn starch 5-40g / L, so...

Embodiment 3

[0041] A kind of preparation method of low-temperature amylase special for livestock and poultry feed of the present embodiment, comprises the following steps:

[0042] 1) Primary slant culture: inoculate the low-temperature amylase-producing bacteria on the slant medium, and culture it at 25°C to 35°C for 4-8 days to obtain the first-grade slant seed spores; the slant medium is a mixture prepared according to the following ratio Solution: sodium nitrate 3.0~5.0g / L, dipotassium hydrogen phosphate 0.5~1.5g / L, magnesium sulfate 0.5~5g / L, potassium chloride 1.0~10g / L, ferrous sulfate 1~5g / L, sucrose 10 ~30g / L, agar 15g;

[0043] 2) Secondary cultivation: inoculate the spores of the primary slant seeds in step 1) into the liquid culture medium, the liquid filling volume is 50-100mL / 500mL, and cultivate at 25°C-35°C, 160-300rpm / min for 48- 55h to obtain secondary seed liquid; maltodextrin 5-20g / L, corn starch 5-40g / L, soybean meal powder 5-20g / L, glucose 3-12g / L, tryptone 10-25g / L...

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Abstract

The invention provides a preparation method for special low-temperature amylase for livestock feedstuff. The preparation method includes the steps that 1, first-stage slant culture is performed; 2, second-stage culture is performed; 3, third-stage enlarged culture is performed; 4, solid fermentation is performed, or the preparation method includes the steps that 1' first-stage slant culture is performed; 2' second-stage slant culture is performed; 3' solid fermentation is performed. A solid fermentation technology is adopted for research and production, the most suitable operation temperature is low, the low-temperature amylase is high in activity at animal body temperature (38 DEG C-42 DEG C), the pH value (4-6) range of special low-temperature amylase exerting the optimal activity is matched with the pH value of livestock gastrointestinal tracts, and the low-temperature amylase has excellent stomach-passing performance, can resist damage of gastric acid and pepsin, is good in heat resistance, can resist high temperature in the granulation process of the feedstuff, is good in storing stability, is efficient incision type amylase, and is an environmentally-friendly feedstuff additive which is free of toxic and side effects, contamination and residues, safe and efficient.

Description

technical field [0001] The invention belongs to the field of feed additives, and in particular relates to a preparation method of low-temperature amylase special for livestock and poultry feed. Background technique [0002] The digestion, absorption and metabolism of nutrients by livestock and poultry are all carried out under the action of enzymes. Feed enzyme preparation is a natural, green, safe and efficient new feed additive produced by microbial fermentation. It has many important biological functions for livestock and poultry, and the most important and direct function is to help livestock and poultry improve Digestion and utilization of nutrients. Adding exogenous digestive enzymes to feed can promote the digestion, absorption and utilization of feed nutrients by livestock and poultry, which has been confirmed by a large number of experiments. Amylase is the key enzyme in digestive enzymes. The digestion and utilization of starch by livestock and poultry mainly dep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/26A23K20/189
CPCC12N9/2411
Inventor 王云龙吴勃高研
Owner HANGZHOU BIOCOM BIOLOGICAL TECH
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