Method for simultaneously detecting two mycotoxins through magnetic control electrochemical adapter sensor

An aptamer sensor and mycotoxin technology, applied in the field of electrochemical detection, can solve the problems of single detection, low sensitivity and long detection time, etc.

Inactive Publication Date: 2016-03-23
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The present invention aims at inventing a magneto-controlled electrochemical aptamer sensor with the advantages of high throughput, high selectivity, simple operation, and low cost, and provides a simple preparation process, high sensitivity, wide

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  • Method for simultaneously detecting two mycotoxins through magnetic control electrochemical adapter sensor
  • Method for simultaneously detecting two mycotoxins through magnetic control electrochemical adapter sensor

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[0039] Example 1

[0040] (1) Preparation of MB-aptamer1 and MB-aptamer2 capture probes:

[0041] Prepare Fe according to existing methods 3 O 4 Microspheres, specifically as follows: 0.5536gFeCl 3 ·6H 2 O was dissolved in a mixture of 10mL of ethylene glycol (EG) and 10mL of diethylene glycol (DEG), then 1.5g of sodium acetate and 1.0g of polyethylene glycol were added, stirred at room temperature for 30min, and transferred to the hydrothermal reactor React at 200°C for 2.5h, then cool to room temperature. The mixture is magnetically separated and washed with water and ethanol several times to obtain Fe 3 O 4 Microspheres, put in an oven, volatilize the solvent at 50℃ to obtain dry Fe 3 O 4 Microspheres, spare.

[0042] Prepare Fe coated with gold nanoparticles according to the existing method 3 O 4 Microspheres (MB), specifically as follows: 0.14g of Fe prepared above 3 O 4 The microspheres were dissolved in 40 mL of ethanol, and 400 μL of 3-aminopropyltriethoxysilane (APTS) stock...

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Abstract

The invention provides a method for simultaneously detecting two mycotoxins through a magnetic control electrochemical adapter sensor. The method comprises the following steps: preparing an aqueous dispersion of Fe3O4 microballoons coated with gold nanoparticles; preparing a water-soluble cadmium telluride quantum dot solution and a water-soluble lead sulfide quantum dot solution; preparing a monodisperse silanization SiO2 nanoparticle aqueous dispersion; preparing the aqueous dispersion of SiO2 nano-balloons coated with cadmium telluride quantum dots and the aqueous dispersion of SiO2 nano balloons coated with lead sulfide quantum dots; preparing MB-aptamer1 and MB-aptamer2 capturing probes; coupling the complementary chain of the adapter 1 with SiO2@PbS, thereby acquiring cDNA1-SiO2@PbS; coupling the complementary chain of the adapter 2 with SiO2@CdTe, thereby acquiring cDNA1-SiO2@CdTe nanometer biological probe; preparing MB-aptamer1/DNA1-SiO2@PbS and MB-aptamer2/DNA2-SiO2@CdTe nanometer biological compound material; constructing the sensor and detecting the sample. The screen-printed electrode adopted in the method provided by the invention is simple in structure; the processing method is simple and convenient; the cost is low; the method has the characteristics of operation convenience of detection method, high detection sensitivity, and the like.

Description

technical field [0001] The invention belongs to the field of electrochemical detection, in particular to a construction method and application of a magnetically controlled electrochemical aptamer sensor for simultaneously detecting two mycotoxins. Background technique [0002] Ochratoxin A (ochratoxinA, OTA) is a kind of mold nephrotoxin produced by Aspergillus chraceus and Penicillium viridicatum, which is a typical food-borne mycotoxin. OTA can exist stably in polar organic solvents. For example, the ethanol solution of OTA can exist stably for more than one year under refrigerated conditions, but OTA will decompose soon under ultraviolet irradiation. In animals, OTA is very stable and not easily degraded by metabolism, and OTA is toxic in many kinds of animals. Studies have found that its toxicity mainly manifests as renal toxicity, liver toxicity, immunotoxicity, teratogenic toxicity and carcinogenicity. In 1993, OTA was classified as a Class 2B carcinogen by the Intern...

Claims

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Application Information

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IPC IPC(8): G01N27/327B82Y30/00B82Y40/00
CPCB82Y30/00B82Y40/00G01N27/3277G01N27/3278
Inventor 王成全钱静王坤黄星奕华梦娟刘倩郝楠
Owner JIANGSU UNIV
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