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Metabolic marker for diagnosis of coronary atherosclerosis

A technology of coronary atherosclerosis and metabolic markers, applied in the field of biochemistry, to achieve the effect of improving the convenience of diagnosis, accurate and reliable results, and promoting standardization

Active Publication Date: 2016-03-23
苏州帕诺米克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Coronary atherosclerosis has not been diagnosed using plasma metabolite levels

Method used

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  • Metabolic marker for diagnosis of coronary atherosclerosis
  • Metabolic marker for diagnosis of coronary atherosclerosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Screening and Characterization of Differential Metabolites in Plasma Between Patients with Coronary Atherosclerosis and Healthy Individuals

[0026] 1. Objects and methods

[0027] 1. The source of the specimen

[0028] After obtaining the consent of the patients, the peripheral venous blood plasma of 480 patients with coronary atherosclerosis and 350 healthy people in Jiangsu Provincial People's Hospital from September 2010 to June 2015 was collected. All patients or healthy people were confirmed by coronary angiography. The age and sex of healthy people were matched with patients with coronary atherosclerosis. All patients with coronary atherosclerosis and healthy people have normal heart, lung, liver, kidney and hematopoietic functions.

[0029] The time of blood collection was in the morning on an empty stomach.

[0030] 2. Main reagents

[0031] Acetonitrile and formic acid (UPLC pure) were purchased from ROE Company in the United States; chromatogra...

Embodiment 2

[0052] Example 2: Constructing a ROC curve to compare the ability of 11 differential metabolites to diagnose and distinguish coronary atherosclerosis patients from healthy people

[0053] The receiver operating curve (ROC) method was used to verify the ability of diagnosing coronary atherosclerosis by the expression levels of 11 differential metabolites in the plasma of patients with coronary atherosclerosis and healthy people. The results showed that 1-arachidonoyl-2-hydroxy-sn-glycero-3-phosphatidylethanolamine, 1-eicosenoyl-2-hydroxy-sn-glycerol-3-phosphatidylethanolamine, 1-oleoyl -sn-glycerol-3-phosphatidylcholine, 1-docosahexaenoyl-2-hydroxy-sn-glycerol-3-phosphatidylethanolamine, 1-docosapentaenoyl-2-hydroxy- sn-glycerol-3-phosphatidylethanolamine, 1-docosapentaenoyl-sn-glycero-3-phosphatidylcholine, 1-stearoyl-sn-glycero-3-phosphatidylcholine, 1-olein Acyl-2-hydroxy-sn-glycerol-3-phosphatidylethanolamine, 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphatidylethanolamine, 1...

Embodiment 3

[0064] Example 3: Application in Screening Drugs for Treatment or Alleviation of Coronary Atherosclerosis

[0065] The plasma of 155 patients with coronary atherosclerosis before and after drug treatment was detected, and the results showed that: with the increase of the course of drug treatment, the patient's condition was gradually relieved, and the concentration of 11 metabolic markers provided by the present invention in plasma The level tends to the level of healthy people gradually, and there is no significant difference between the plasma level of healthy people after complete cure (P>0.05). This result is consistent with the detection results of coronary angiography. Therefore, the metabolic markers provided by the present invention can be used to screen drugs for treating or alleviating coronary atherosclerosis.

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Abstract

The invention discloses a metabolic marker for diagnosis of coronary atherosclerosis. The metabolic marker includes one or more of 1-arachidonoyl-2-hydroxy-sn-glycerol-3-phosphatidylethanolamine, 1-eicosenoyl-2-hydroxy-sn-glycerol-3-phosphatidylethanolamine, 1-oleoyl-sn-glycerol-3-phosphatidylcholine, 1- docosahexanoyl-2-hydroxy-sn-glycerol-3-phosphatidylethanolamine, 1-docosapentanoyl-2-hydroxy-sn-glycerol-3-phosphatidylethanolamine, 1-docosapentanoyl-sn-glycerol-3-phosphatidylcholine, 1-stearoyl-sn-glycerol-3 phosphatidylcholine, 1-oleoyl-2-hydroxy-sn-glycerol-3-phosphatidylethanolamine, 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphatidylethanolamine, 1-palmitoyl-sn-glycerol-3-phosphatidylcholine, and 1-octadecanoyl-2-hydroxy-sn-glycerol-3-phosphatidylethanolamine. The metabolic marker provided by the invention can accurately diagnose and distinguish coronary atherosclerosis patients and healthy people, and has high accuracy, strong sensitivity and specificity.

Description

technical field [0001] The invention belongs to the field of biochemistry, relates to disease diagnosis markers, in particular to a group of metabolic markers for diagnosing coronary atherosclerosis. Background technique [0002] Coronary atherosclerosis is a common progressive arterial disease. The lesions mainly involve medium-sized muscular arteries. Lipid deposition in the arterial intima, proliferation of smooth muscle cells, and the formation of localized plaques can harden the arterial wall. Severe When plaque rupture leads to thrombus, embolism, hemorrhage, partial or complete occlusion of the involved lumen, the clinical manifestation is the occurrence of atherosclerotic vascular complications. The most common and serious vascular complication in elderly patients is coronary heart disease (unstable angina pectoris and myocardial infarction) is the main cause of coronary heart disease. Early lesions of atherosclerosis can occur before the age of 10, and it takes 20 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N33/92G01N30/02
Inventor 齐炼文赵艺静范勇朱伟李萍陈彦
Owner 苏州帕诺米克生物科技有限公司
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