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Application of endophytic bacteria b29 in Sophora japonica in the control of Panax notoginseng black spot

A technology of Panax notoginseng black spot and endophytic bacteria, applied in bacteria, applications, microorganism-based methods, etc., can solve the problems of pollution, threat to health, economic loss of farmers, etc., and achieve broad application prospects and strong inhibitory effects. Effect

Active Publication Date: 2019-02-26
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional authentic production area of ​​Sanqi in Guangxi, due to the low altitude and high temperature and humidity weather conditions, this disease often occurs at the same time, resulting in a large amount of production reduction or even failure of Sanqi, and brought huge economic losses to the growers.
In order to control this fungal disease, a large amount of chemical pesticides are used, resulting in a large amount of pesticide residues in Panax notoginseng products, seriously polluting the environment, and greatly threatening people's health

Method used

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  • Application of endophytic bacteria b29 in Sophora japonica in the control of Panax notoginseng black spot
  • Application of endophytic bacteria b29 in Sophora japonica in the control of Panax notoginseng black spot
  • Application of endophytic bacteria b29 in Sophora japonica in the control of Panax notoginseng black spot

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Isolation, screening and identification of strains

[0023] 1. Isolation of strains

[0024] Test material: wild Vietnamese pagoda tree collected from the limestone area of ​​Tiandeng County, Guangxi.

[0025] Tested strains: provided by Institute of Plant Pathology, Guangxi University.

[0026] Medium: 1000ml NA medium: beef extract 3g, yeast extract 1g, peptone 5g, sucrose 10g, agar 15g, pH 7.0.

[0027] Surface disinfection: Rinse the fresh and healthy Vietnamese Sophorae root with a length of 6-8cm and a width of 1-2cm with running water for 30 minutes to wash away the sediment, then rinse the washed Vietnamese Sophorae root with sterile water twice, and air-dry the surface moisture , moved to the ultra-clean workbench, and under aseptic conditions, the Sophora japonica root was soaked in 75% ethanol for 1 min, rinsed with sterile water twice, soaked in sodium hypochlorite (available chlorine 1%) for 2 min, washed with sterile water for 3 times , dry the surface ...

Embodiment 2

[0079] Inhibitory effect of metabolites of endophytic bacteria B29 from Sophora vietnamese against Panax notoginseng black spot

[0080] 1. Fermentation of strains and extraction of metabolites

[0081] Inoculate the endophytic bacteria B29 of Sophora vietnamese into a 2-liter Erlenmeyer flask containing 1000ml NB liquid medium (3g of beef extract, 1g of yeast extract, 5g of peptone, 10g of sucrose, pH 7.0) 130r / min shaking table fermentation culture for 10 days, the obtained fermented product was ultrasonically ultrasonicated for 40min, and then extracted twice with ethyl acetate, and the obtained ethyl acetate phase was concentrated under reduced pressure twice to obtain the crude ethyl acetate extraction of the fermented product of the strain The product is the metabolite of endophytic bacteria B29 in Sophora vietnamese.

[0082] 2. Inhibition of the metabolites of endophytic bacteria B29 from Sophora vietnamese on the mycelial growth of Panax notoginseng black spot fungus...

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Abstract

The invention discloses an endophytic bacterium B29 of Sophora japonica. The classification of the endophytic bacterium B29 of Sophora japonica is named as Burkholderia sp. B29, and the 16S rDNA gene sequence table of the strain is shown as SEQ ID NO.1 As stated, depository unit: General Microbiology Center of China Microbial Culture Collection Management Committee, deposit address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, deposit date: May 13, 2015, deposit number : CGMCC No.10807. In the present invention, for the first time, an endophytic bacterium (Burkholderia sp.) B29 was isolated and screened from the root of the medicinal plant Sophora japonica. field has broad application prospects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of an endophytic bacterium B29 of Sophora japonica in the prevention and treatment of Panax notoginseng black spot. Background technique [0002] The prevention and control of plant diseases in modern agriculture is overly dependent on the use of chemical pesticides. The application of a large number of chemical pesticides not only has long-term damage to the ecological environment, but also causes the quality of agricultural products to decline, pesticide residues exceed the standard, pathogenic bacteria are resistant to drugs, and are harmful to humans and animals. Many questions. It is of great significance to find safer and more effective methods of disease control, and the use of biological methods to prevent and control plant diseases can effectively solve the above problems. [0003] Sanqi (Panax notoginseng F.H.chen), also known as Tianqi, Jinbuhuan, etc., ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04A01N63/02A01P3/00C12R1/01
CPCA01N63/10C12P1/04C12N1/205C12R2001/01
Inventor 黄荣韶姚裕群蓝芳李良波
Owner GUANGXI UNIV