Application of At1g52340 gene of arabidopsis in control over plant leaf ageing

A plant leaf, Arabidopsis technology, applied in the directions of botanical equipment and methods, applications, plant products, etc., can solve the problem of negative regulation of leaf senescence and no relevant reports have been reported.

Inactive Publication Date: 2016-04-06
NANYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The length of the CDS of this gene is 858 bases, which encodes a protein of 252 amino acids. Studies have shown that it is related to the germination of plant seeds, plant development and the movement of stomata, but there are few reports on the senescence of plant leaves. However, there is no relevant report on the negative regulation of leaf senescence mediated by it.

Method used

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  • Application of At1g52340 gene of arabidopsis in control over plant leaf ageing
  • Application of At1g52340 gene of arabidopsis in control over plant leaf ageing
  • Application of At1g52340 gene of arabidopsis in control over plant leaf ageing

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Arabidopsis At1g52340 gene is associated with leaf senescence

[0025] refer to figure 1 , Arabidopsis wild-type (wildtype, WT) and mutant (eas1-1) seeds were inoculated in 0.8% agar MS medium, cultured in light, the light / dark cycle was 16 / 8h, the temperature was 22°C, relative The humidity was 70%, WT and eas1-1 seeds germinated and grew for 7 days, then transferred to nutrient soil and placed in the greenhouse for cultivation. The maximum photochemical rate (Fv / Fm) and chlorophyll content of the leaves of WT and eas1-1 plants grown in different periods were measured, and the results showed that compared with the leaves of WT plants, the leaves of mutant eas1-1 had higher chlorophyll content and higher photosynthetic efficiency, the chlorophyll content and photosynthetic efficiency decreased significantly after 30 days ( figure 1 A-C), Premature senescence of leaves. In addition, compared with WT plants of WT and eas1-1 plants normally grown in soil, the leaf senes...

Embodiment 2

[0028] Arabidopsis At1g52340 gene regulates leaf senescence affected by osmotic and oxidative stress

[0029] refer to figure 2 , using artificial adversity stress to treat the isolated fifth leaves of WT and eas1-1 mutants with osmotic and oxidative stress, and detect their senescence phenotype, chlorophyll content and ion leakage of leaves. The data showed that compared with the WT, the leaves of the eas1-1 mutant showed significantly earlier symptoms of senescence, such as yellowing and excessive death spots ( figure 2 A); the DNA content of leaves also decreased significantly faster ( figure 2 B). The data of chlorophyll content and ion leakage also supported this view. With the prolongation of the stress time, the chlorophyll content of mutant eas1-1 detached leaves decreased significantly faster, while the ion leakage increased significantly ( figure 2 C-F).

[0030] The above results indicated that the Arabidopsis At1g52340 gene was involved in the regulation of...

Embodiment 3

[0032] Cytological Evidence for the Regulation of Leaf Senescence by the Arabidopsis At1g52340 Gene

[0033] refer to image 3 , by comparing the physiological evidence of leaf senescence staining and leaf cell death, it was found that the leaves of the mutant eas1-1 had a larger and more obvious area of ​​decay spots than WT leaves under the condition of trypan blue staining ( image 3 A) DAPI and PI staining can more clearly show that the leaves of mutant eas1-1, whether it is mesophyll cells, leaf epidermal cells or guard cells, all show the phenomenon that the cell membrane is destroyed earlier ( image 3 B).

[0034] In order to further illustrate the senescence of the leaf cells of eas1-1, the present invention analyzes the cell loss of the mesophyll cells, observes and counts their apoptosis rate, and the results show that after 25 days, the apoptosis of the leaf cells of eas1-1 The rate was significantly higher than that of WT ( image 3 C-D).

[0035] It can be pr...

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Abstract

The invention relates to application of an At1g52340 gene of arabidopsis in control over plant leaf ageing, and belongs to the technical field of gene engineering. The At1g52340 gene is connected to different kinds of vectors, transgenic crops related to ageing resisting are obtained through conversion of the different expression vectors, the CDS length of the At1g52340 gene is 858 basic groups, a protein formed by 252 amino acids is encoded, and dehydrogenization reductase positioned in cell cytoplasm is encoded. It is proved that the At1g52340 gene of the arabidopsis can influence plant ageing by regulating expression of the gene related to plant ageing and play a role on regulating ageing and apoptosis of plant leaf cells at a cellular level, and the regulating mechanism also participates in response of plants to adverse situations such as permeation and oxidative stress. Through a transgenic technology, the gene can be used for performing crop breeding, premature aging of the crops is delayed, and yield increasing and harvesting are achieved.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to an application of Arabidopsis thaliana At1g52340 gene in regulating plant leaf senescence. Background technique [0002] Leaf is an important organ for photosynthesis, synthesis of organic matter and increase of crop yield. Premature senescence of plant leaves often leads to crop yield reduction or even infertility. The senescence of plant leaves is regulated by growth environment and internal plant hormone signals. Numerous studies have shown that the plant hormone abscisic acid (ABA) is an important stress hormone produced by plants in response to external environmental stress, which can improve plant resistance to stress (such as drought, high temperature, cold damage, salinity, etc.). ABA has long been known to promote leaf senescence because ABA is present in higher levels in senescent leaves compared to young leaves and spraying ABA on leaves can p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00
CPCC12N15/8261
Inventor 宋玉伟向福友苗雨晨宋纯鹏
Owner NANYANG NORMAL UNIV
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