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A kind of preparation method of L-malic acid

A technology of malic acid and calcium carbonate, applied in the direction of fermentation, etc., can solve the problems of environmental pollution, large amount of calcium sulfate solid waste, etc., and achieve the effect of reducing production cost, high cost and saving cost

Active Publication Date: 2020-09-04
ANHUI XUELANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to: provide a green production method of L-malic acid, solve the problems of large solid waste of calcium sulfate and serious environmental pollution in the preparation of malic acid, so as to achieve the purpose of green industrial production

Method used

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  • A kind of preparation method of L-malic acid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A preparation method of L-malic acid, the steps are as follows:

[0035] 1) Preparation of enzyme solution

[0036] Mix 10g of malonic acid, 15g of diammonium hydrogen citrate, 0.2g of magnesium sulfate, 1g of potassium dihydrogen phosphate, and 25g of corn steep liquor, adjust the pH to 7.25 with 20% sodium hydroxide, add water to 1000ml, and pack to Erlenmeyer flask: Sterilize the Erlenmeyer flask at 121°C for 30 minutes. After the temperature drops, insert Brevibacterium flavum and culture on a shaking table for 32-36 hours to obtain enzyme solution I, whose pH is between 6.5 and 7.5.

[0037] 2) Enzyme conversion

[0038] In a stirred reactor, 232g of fumaric acid and 200g of calcium carbonate were stirred and reacted in an aqueous solution at 80°C for 1 hour, and the pH was controlled between 6.5 and 7.0 during the reaction to generate 308g of calcium fumarate;

[0039] In a conversion tank with stirring, add the enzyme liquid I prepared in step 1) to the obtaine...

Embodiment 2

[0046] A preparation method of L-malic acid, the steps are as follows:

[0047] 1) Preparation of enzyme solution

[0048] Mix 5g of malonic acid, 20g of diammonium hydrogen citrate, 0.1g of magnesium sulfate, 0.5g of potassium dihydrogen phosphate, and 20g of corn steep liquor, adjust the pH to 7.48 with 20% sodium hydroxide, add water to 1000ml, and pack Transfer to the Erlenmeyer flask; sterilize the Erlenmeyer flask at 121°C for 30 minutes. After the temperature drops, insert Brevibacterium flavum and culture on a shaking table for 32-36 hours to obtain enzyme solution II, whose pH is between 7.5 and 8.5.

[0049] 2) Enzyme conversion

[0050] In a stirred reactor, 232g of fumaric acid and 200g of calcium carbonate were stirred and reacted in an aqueous solution at 90°C for 2 hours. During the reaction, the pH was controlled between 7.5 and 8.0 to generate 308g of calcium fumarate;

[0051] In the conversion tank with stirring, add the enzyme solution II prepared in step...

Embodiment 3

[0058] A kind of preparation method of L-malic acid, such as figure 1 As shown, the steps are as follows:

[0059] 1) Preparation of enzyme solution

[0060] Mix 15g of malonic acid, 10g of diammonium hydrogen citrate, 0.3g of magnesium sulfate, 2g of potassium dihydrogen phosphate, and 30g of corn steep liquor, adjust the pH to 7.45 with 20% sodium hydroxide, add water to 1000ml, and pack to Erlenmeyer flask: Sterilize the Erlenmeyer flask at 121°C for 30 minutes. After the temperature drops, insert Brevibacterium flavum and culture on a shaking table for 32-36 hours to obtain enzyme solution III, whose pH is between 7.5 and 8.5.

[0061] 2) Enzyme conversion

[0062] In a stirred reactor, 464g of fumaric acid and 400g of calcium carbonate were stirred and reacted in an aqueous solution at 85°C for 3 hours, and the pH was controlled between 7.5 and 8.0 during the reaction to generate 616g of calcium fumarate;

[0063] In the conversion tank with stirring, add the enzyme so...

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Abstract

The invention provides a preparation method of L-malic acid. The preparation method comprises steps as follows: firstly, fumaric acid and calcium carbonate react to generate calcium fumarate, and generated calcium fumarate is converted into calcium malate under the action of fumarase; secondly, calcium malate and carbonate react in an aqueous solution, and a malate solution is generated; finally, the generated malate solution is converted into a malic acid solution with an electrodialysis method, and L-malic acid is obtained. According to the method, sulfuric acid is not utilized, no byproducts are produced, the method is environment-friendly, an extraction process is simple, intermediate products can be recycled, the cost is saved, and the enzymatic conversion rate is higher than 99.9%.

Description

technical field [0001] The invention belongs to the technical field of food industry raw material production, relates to a green production method of L-malic acid, and particularly relates to the improvement of refining technology. Background technique [0002] L-malic acid, also known as hydroxysuccinic acid, is a very important organic acid, which widely exists in various organisms such as animals, plants and microorganisms. It has important physiological functions in organisms and can be used in food, Daily chemical and pharmaceutical industries and other fields. [0003] In the prior art, the artificial preparation of L-malic acid is mainly realized by various methods such as microbial fermentation method, traditional enzyme immobilization method and integrated technology (reaction and separation coupling technology). However, these methods have limitations to varying degrees. When L-malic acid is prepared by microbial fermentation, the acid production and conversion r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/42C12P7/46
CPCC12P7/42C12P7/46
Inventor 李云政李正华万玉青孟庆强程晋超刘二丹
Owner ANHUI XUELANG BIOTECHNOLOGY CO LTD
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