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Method for extracting tigogenin and/or hecogenin with composite bacteria method

A technology of sisal saponin and compound bacteria, which is applied in the field of extracting sisal saponin and/or hecogenin, can solve the problems of low product yield and purity, environmental pollution toxicity, complicated process, etc., and achieves product yield and purity. The effect of improving, high economic efficiency and simple process operation

Active Publication Date: 2016-04-20
北京颐方生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of low product yield and purity in the existing methods for extracting sisal saponin and hecoginine, the use of strong acid, strong base or organic solvent not only pollutes the environment but also has high toxicity, complicated process and high cost. The invention provides a method for extracting sisal saponin or sea cochinine by compound bacteria method

Method used

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  • Method for extracting tigogenin and/or hecogenin with composite bacteria method
  • Method for extracting tigogenin and/or hecogenin with composite bacteria method
  • Method for extracting tigogenin and/or hecogenin with composite bacteria method

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Experimental program
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Effect test

Embodiment 1

[0030] (1) Take 50Kg of sisal dry residue, add 100Kg of water, adjust the pH to 4.5 with citric acid-sodium hydroxide-hydrochloric acid buffer solution, add 500g of pectinase and 500g of cellulase, and stir for 4-5h to obtain a clear liquid 1.

[0031] (2) Appropriate amounts of Aspergillus niger and Aspergillus oryzae were inoculated into respective culture solutions (each 100 L) and fermented until the bacteria amount reached 15 mg / mL. The two fermentation broths were mixed with clear liquid 1, and stirred and reacted at a temperature of 37° C. for 2 days. Stand still for 48 hours, remove the supernatant, centrifuge the lower layer at 4000 rpm, remove the supernatant to obtain a total of 9.42Kg of precipitate 1, collect the supernatant and discard it after high-temperature sterilization.

[0032] (3) Mix the precipitate 1 with 1.884Kg quicklime, add 94.2Kg85% ethanol aqueous solution, heat and reflux at 79°C for 4h, add 1.413Kg gac, after reflux for 2h, centrifuge at 4500 r...

Embodiment 2

[0039] (1) Take 50Kg of sisal dry residue, add 100Kg of water, adjust the pH to 4.5 with citric acid-sodium hydroxide-hydrochloric acid buffer solution, add 500g of pectinase and 500g of cellulase, and stir for 4-5h to obtain a clear liquid 1.

[0040] (2) Inoculate an appropriate amount of Trichoderma reesei (Trichordermareesei), Aspergillus oryzae (Aspergillus oryzae) and Penicillium sp. into respective culture fluids respectively, wherein the culture fluid of Trichoderma reesei and Aspergillus oryzae is 100L, Penicillium sp. 50L of culture solution, fermented until the amount of bacteria is 15mg / mL. The three fermentation broths were evenly mixed with clear liquid 1, and stirred and reacted at a temperature of 37° C. for 2 days. Stand still for 48 hours, remove the supernatant, centrifuge the lower layer at 4000 rpm, remove the supernatant to obtain a total of 10.6Kg of precipitate 1, collect the supernatant and discard it after high-temperature sterilization.

[0041] (3...

Embodiment 3

[0047] 3.1 Replace Aspergillus niger+Aspergillus oryzae in embodiment 1 with Aspergillus niger

[0048] (1) Take 50Kg of sisal dry residue, add 100Kg of water, adjust the pH to 4.5 with citric acid-sodium hydroxide-hydrochloric acid buffer solution, add 500g of pectinase and 500g of cellulase, and stir for 4-5h to obtain a clear liquid 1.

[0049] (2) An appropriate amount of Aspergillus niger was inoculated into the culture medium (200 L) and fermented until the bacteria amount was 15 mg / mL. The supernatant liquid 1 was mixed with the fermentation broth, and stirred and reacted at a temperature of 37° C. for 2 days. Stand still for 48 hours, remove the supernatant, centrifuge the lower layer at 4000 rpm, remove the supernatant to obtain a total of 6.32Kg of precipitate 1, collect the supernatant and discard it after high-temperature sterilization.

[0050] (3) Mix the precipitate 1 with 1.264Kg quicklime, add 63.2Kg85% ethanol aqueous solution, heat and reflux at 79°C for 4...

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Abstract

The invention discloses a method for extracting tigogenin and / or hecogenin with a composite bacteria method. The method comprises steps as follows: pectin in agave sisal dregs is hydrolyzed with pectinase and cellulase, saponin in the sisal dregs is released while the viscosity of the sisal dregs is reduced, and then the released saponin is effectively degraded by the aid of compound enzyme produced from mould composition after fermentation. The method can be used for extracting tigogenin or hecogenin from the sisal dregs, can be used for extracting tigogenin and hecogenin from the sisal dregs simultaneously, and has the advantages of simplicity in operation, mild extraction conditions, small environmental pollution, high product yield and purity, low cost and the like.

Description

technical field [0001] The invention relates to the field of production of pharmaceutical raw materials, in particular to a method for extracting sisal saponin and / or hecoginine. Background technique [0002] Sisal, also known as pineapple hemp, sisal hemp, and agave hemp, is a plant of the genus Agave in the family Agaveaceae. According to statistics, at the end of 2010, the planting area of ​​sisal in my country was 20,500 hectares, the harvested area was 18,300 hectares, and the total fiber output was 46,300 tons. The weight of dry fiber in sisal accounts for about 6% of the whole plant, and more than 90% of the other is leaf juice, leaf residue, hemp stem, and flower axis. At present, the most important use of sisal is to extract sisal fiber. During the processing, a large amount of hemp residue will be produced, which will not only cause waste of resources, but also cause environmental pollution. In order to solve this problem, domestic scientific researchers have don...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P33/20C12R1/685C12R1/69
CPCC12P33/20C12P39/00
Inventor 石清东王姣
Owner 北京颐方生物科技有限公司
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