GC-Q-TOF/MS detection technology of 708 pesticide residues in root vegetables and tuber vegetables

Abstract

The invention relates to the field of detection of pesticide residues, and provides a detection method for screening 708 pesticide residues in root vegetables and tuber vegetables. The method comprises the steps of collecting full-scan mass spectrum of fragment ions under specified requirements of pesticide standards in a GC-Q-TOF / MS first-class mode and obtaining chemical formula, retention time, accurate mass number of fragment ions and ion abundance ratio of a pesticide, importing PCDL software, correlating with the corresponding pesticide information to form a database, measuring full spectrum data of a sample to be detected under the same setting condition, so as to obtain the full spectrum data; performing accurate quality database retrieval on a sample determine result, accurately identifying the detected pesticide accorded with the identification basis, so as to obtain the types of pesticide residues in the in root vegetables and tuber vegetables. A detection method established by a GC-Q-TOF / MS high resolution mass spectrum is adopted to detect the pesticide residues in the root vegetables and the tuber vegetables, specific pretreatment is preferably combined, and the characteristics of speediness, high throughput, high precision and high reliability detection are achieved. A standard substance as contrast is not required, and meanwhile the 708 pesticides in the root vegetables and the tuber vegetables are subjected to qualitative detection and confirmation.

Description

technical field [0001] The invention relates to the field of pesticide residue detection, in particular to a detection method for screening 708 kinds of pesticide residues in rhizome and yam vegetables by using GC-Q-TOF / MS. Background technique [0002] Fruits and vegetables are closely related to people’s lives, especially the nutritional value of root and tuber vegetables (potatoes, radishes, carrots, taro, purple potatoes, bellflowers, sweet potatoes, yacon, ginger and yams, etc.) are favored by consumers . This kind of vegetable is mainly starch, with high sugar content, such as purple sweet potato, taro, potato, yam, etc., which can partially replace the staple food. Other nutrients are different, such as iodine, bromine, amylase in radish; potassium salt and vitamin C in potato; high carotene in carrot. However, at present, there are more than 1100 kinds of pesticides and chemicals that may be used in the planting, growth and preservation of rhizome and yam vegetable...

AI Technical Summary

Problems solved by technology

In the actual work of pesticide and veterinary drug residue laboratories, most laboratories do not store hundreds of pesticide standard products. The reason is that pesticide standard products are not only expensive, but also valid for only 2-3 years, requiring repeated investment.

There are only dozens of standard pesticides in general laboratories, and the types of pesticides that are routinely monitored are limited to these dozens, resulting in loopholes in food safety monitoring

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