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A kind of microbial film bacterial agent for degrading polycyclic aromatic hydrocarbons and preparation method thereof

A technology for microbial membranes and polycyclic aromatic hydrocarbons, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as quantity or activity attenuation or even disappearance, slow initiation of biochemical reactions, and inability to ensure repair efficiency, etc. Achieve the effect of fast growth, convenient operation and short fermentation period

Active Publication Date: 2019-05-28
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because exogenous functional bacteria are not suitable for site conditions and complex natural environment, it is difficult to compete with native bacteria, and their number or activity usually rapidly decays or even disappears; the bacteria are easy to lose; the concentration of effective bacteria is low; resulting in slow start-up of biochemical reactions; Poor environmental tolerance; ultimately does not ensure consistently high remediation efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1-1. Preparation of microbial film agent

[0024] The bacterial strain Mycobacterium sp.NJS-P is activated, and then inoculated in 250mL inorganic salt medium, with a concentration of 3g L -1 The phenanthrene is the carbon source, at a temperature of 30°C and a rotational speed of 160r min -1 Conditioned for 72h. Then use inorganic salts to clean and adjust the OD 600 Value to 0.7, the volume is about 200mL. Then, transfer it to the fermenter according to the ratio of 1:50, maintain the temperature at 30°C, the pressure at 0.05MPa, and the ventilation rate at 0.4V V -1 min -1 , stirring speed 300rpm min -1 , ferment for 15 hours to obtain the microbial fermentation bacteria suspension. Then, take 100g of humin and bake at 280°C for 2h, add 300mL of microbial fermentation suspension after cooling, then shake and culture for 12h, the culture temperature is 28°C, and the speed is set at 100r min -1 , and finally obtain the microbial film inoculant.

[0025] 1-2. De...

Embodiment 2

[0030] 2-1. Preparation of microbial film agent

[0031] Biofilm agent preparation process: the bacterial strain Mycobacterium sp.NJS-1 is activated, then inoculated in 250mL inorganic salt medium, with a concentration of 3g L -1 The phenanthrene is the carbon source, at a temperature of 30°C and a rotational speed of 160r min -1 Conditioned for 72h. Then use inorganic salts to clean and adjust the OD 600 Value to 0.7, the volume is about 200mL. Then, transfer it to the fermenter according to the ratio of 1:100, maintain the temperature at 30°C, the pressure at 0.03MPa, and the ventilation rate at 0.4V V -1 min -1 , stirring speed 300rpm min -1 , ferment for 15 hours to obtain the microbial fermentation bacteria suspension. Then, take 100g of humin and bake at 280°C for 3h, add 400mL of microbial fermentation suspension after cooling, then shake and culture for 12h, the culture temperature is 28°C, and the speed is set at 100r min -1 , and finally obtain the microbial f...

Embodiment 3

[0037] 3-1. Preparation of microbial film agent

[0038] Biofilm agent preparation process: the bacterial strain Sphingobium sp.PHE3 is activated, then inoculated in 250mL inorganic salt medium, with a concentration of 3g L -1 The phenanthrene is the carbon source at a temperature of 28°C and a rotational speed of 160r min -1 Conditioned for 24h. Then use inorganic salts to clean and adjust the OD 600 Value to 0.8, the volume is about 200mL. Then, transfer it to the fermenter according to the ratio of 1:50, maintain the temperature at 28°C, the pressure at 0.03MPa, and the ventilation rate at 0.4V V -1 min -1 , stirring speed 300rpm min -1 , ferment for 15 hours to obtain the microbial fermentation bacteria suspension. Then, take 100g of humin and bake at 280°C for 2h, add 300mL of microbial fermentation suspension after cooling, then shake and culture for 12h, the culture temperature is 28°C, and the speed is set at 100r min -1 , and finally obtain the microbial film i...

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Abstract

The invention relates to an efficient microbial film inoculant for degrading polycyclic aromatic hydrocarbon in contaminated soil and a preparation method of the microbial film inoculant. Humin is adopted as a carrier, microorganisms adhere to the carrier, a microbial film is formed, and the microbial film inoculant is obtained. The microbial film inoculant has the advantages of being simple in preparation method, convenient to operate, short in fermentation period, high in growing speed, high in enzyme activity and the like, has the characteristic of efficiently utilizing and degrading polycyclic aromatic hydrocarbon, and provides a biotechnology for efficiently removing polycyclic aromatic hydrocarbon in the contaminated environment.

Description

technical field [0001] The invention relates to a construction technology of a microbial film bacterial agent for degrading polycyclic aromatic hydrocarbons, in particular to a high-efficiency microbial film bacterial agent for degrading polycyclic aromatic hydrocarbons in polluted soil and a preparation method thereof. technical background [0002] With the rapid development of my country's industrialization, agricultural intensification, urbanization, and transportation modernization, the discharge of polycyclic aromatic hydrocarbons (PAHs) has increased sharply, resulting in increasingly serious soil PAHs pollution, which seriously threatens the soil ecological environment, agricultural product safety and environmental protection. Human health, and affect the sustainable development of my country's social economy. Therefore, according to the characteristics of soil pollution, regulating the key processes that affect the soil purification function, and developing PAHs-conta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/02B09C1/10C12R1/265C12R1/32C12R1/01
CPCB09C1/10C12N11/02
Inventor 张银萍张杰朱晓舒黄鹤勇
Owner NANJING NORMAL UNIVERSITY