A single-chip cultivation method of jade fungus in a greenhouse with hanging bags and small holes
A cultivation method, the technology of jade fungus, applied in cultivation, plant cultivation, mushroom cultivation, etc., can solve the problems of low yield of jade fungus, difficulty in ensuring ear shape, loss of commerciality, etc., and achieve good color, increased yield, and high commodity value Effect
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Embodiment 1
[0023] A method for single-sheet cultivation of jade fungus in hanging bag small holes in greenhouses, comprising the following steps:
[0024] (1) Mother seed preparation: transfer the purified bacterial strains to sterilized PDA medium, culture at 24°C for 10 days, and wait until the mycelium is overgrown with the medium for later use;
[0025] (2) Original seed preparation: put the sterilized original seed medium into a propylene bag with a size of 17cm×35cm, sterilize at 0.11MPa, 121°C for 4 hours, cool to room temperature after sterilization, and store under aseptic conditions. Insert the mother species prepared in the step (1) into the original seed medium in the propylene bag, cultivate for 25 days at 24°C, and wait until the hyphae are full; the original seed medium is composed of the following ingredients by weight percentage: Corn cob 77%, wheat bran 20%, soybean flour 2%, gypsum 1%;
[0026] (3) Cultivar preparation: put the sterilized cultivar culture medium into ...
Embodiment 2
[0035] A method for single-sheet cultivation of jade fungus in hanging bag small holes in greenhouses, comprising the following steps:
[0036] (1) Mother seed preparation: transfer the purified bacterial strain to sterilized PDA medium, culture for 13 days at 26°C, and wait until the mycelium is overgrown with the medium for later use;
[0037] (2) Original seed preparation: put the sterilized original seed medium into a propylene bag with a size of 17cm×35cm, sterilize at 0.13MPa, 121°C for 4h, cool to room temperature after sterilization, and store under aseptic conditions. Insert the mother species prepared in the step (1) into the original seed culture medium in the propylene bag, culture at 26°C for 28 days, and wait until the hyphae are full; the original seed culture medium is composed of the following ingredients by weight percentage: Corn cob 77%, wheat bran 20%, soybean flour 2%, gypsum 1%;
[0038] (3) Cultivar preparation: put the sterilized cultivar culture medi...
Embodiment 3
[0047] A method for single-sheet cultivation of jade fungus in hanging bag small holes in greenhouses, comprising the following steps:
[0048] (1) Mother seed preparation: transfer the purified bacterial strain to sterilized PDA medium, culture for 15 days at 28°C, and wait until the mycelium is overgrown with the medium for later use;
[0049] (2) Original seed preparation: put the sterilized original seed medium into a propylene bag with a size of 17cm×35cm, sterilize at 0.15MPa, 121°C for 4h, cool to room temperature after sterilization, and store under aseptic conditions. Insert the mother species prepared in the step (1) into the original seed culture medium in the propylene bag, cultivate for 30 days at 28°C, and wait until the hyphae are full; the original seed culture medium is composed of the following ingredients by weight percentage: Corn cob 77%, wheat bran 20%, soybean flour 2%, gypsum 1%;
[0050] (3) Cultivar preparation: put the sterilized cultivar culture me...
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