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Rapid detection method for residual trithion in fruits and vegetables

A detection method and technology of trithion, applied in Raman scattering, material excitation analysis, etc., can solve the problems of easy occurrence of false positives, inability to identify organic phosphorus, etc., and achieve the effects of environmental friendliness, short detection time, and easy operation.

Inactive Publication Date: 2016-05-11
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with existing domestic standards, although the pre-treatment process is simplified and no professional technicians are required, it still uses chromatography for detection, and terminal testing still requires professionals and cannot leave the laboratory
[0005] At present, there are also rapid test cards for organophosphorus pesticides on the market, which use cholinesterase, which is highly sensitive to organophosphate, and a color reagent to make enzyme test paper. Although this method is easy to operate, it is prone to false positives, and can only Determination of organophosphates that are highly sensitive to cholinesterase, unable to identify the specific organophosphate

Method used

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  • Rapid detection method for residual trithion in fruits and vegetables
  • Rapid detection method for residual trithion in fruits and vegetables
  • Rapid detection method for residual trithion in fruits and vegetables

Examples

Experimental program
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Effect test

Embodiment 1

[0023] This embodiment includes the following steps:

[0024] 1) Put the cabbage sample into a container, add extractant and water storage agent, and extract by ultrasonic to obtain the extract; the cabbage sample uses 2g; the ratio of the extractant and water storage agent can be 5-10ml : 0.5~2g, preferably 8ml: 1g, wherein extractant is calculated by volume, and water storage agent is calculated by mass; Described extractant can adopt acetonitrile or acetonitrile and acetic acid, and described acetic acid can be 0.1%~1% of acetonitrile by volume percentage %; the water storage agent can be selected from one of anhydrous magnesium sulfate, anhydrous sodium sulfate, etc.; the time of the ultrasonic extraction can be 2-10 minutes, preferably 5 minutes. Source of cabbage samples: commercially available cabbage spiked with phosphorus trithion.

[0025]2) Put the extract obtained in step 1) into a container with a cover, add anhydrous magnesium sulfate, graphitized carbon and bon...

Embodiment 2

[0028] Similar to Example 1, the difference lies in the source of the fruit and vegetable samples: commercially available Chinese cabbage spiked with trithion samples.

[0029] Trithion test curve in the Chinese cabbage of embodiment 2 sees figure 2 , Raman spectrometer power 280mW, laser wavelength 785nm, integration time 5s.

Embodiment 3

[0031] Similar to Example 1, the difference lies in the source of the fruit and vegetable samples: commercially available orange peels spiked with trithion samples.

[0032] The test curve of trithion in the orange of embodiment 3 is referring to image 3 , Raman spectrometer power 180mW, laser wavelength 785nm, integration time 5s.

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Abstract

The invention discloses a rapid detection method for residual trithion in fruits and vegetables, and relates to fruit and vegetable residue detection. A fruit and vegetable sample is placed into a container, an extracting agent and a water storing agent are added, and ultrasonic extraction is performed to obtain an extracting solution; the extracting solution is placed into a container with a cover, anhydrous magnesium sulfate, graphitized carbon and bonded silica gel PSA are added, standing is performed after oscillation is performed, a supernate is placed into another container with a cover, a low-polarity organic solvent is added, standing is performed for the second time after oscillation is performed for the second time, 0.5-4 ml of the supernate is taken to be placed into a test tube, nitrogen drying is performed, an eluent is added again, oscillation is performed for the third time, the eluent is taken to be placed into a detection cell, metal sol and an inorganic salt flocculating agent are added and uniformly mixed, and detection is performed inside a Raman spectrum instrument detection room. Operation is easy and convenient, and detection can be performed with no professionals; the detection time is short, and rapid screening of large-batch samples can be achieved; the precision is high, and no false positive phenomenon exists; no large amount of organic solvent or toxic reagent is needed, and no damage is caused to environment or body health of a detector.

Description

technical field [0001] The invention relates to the detection of pesticide residues in fruits and vegetables, in particular to a rapid detection method for trithion residues in fruits and vegetables. Background technique [0002] There are many kinds of organophosphorus pesticides, which are highly toxic and enter the human body mainly through skin contact, inhalation and direct consumption. At present, there are hundreds of organophosphorus pesticides registered in the world, and dozens of organophosphorus pesticides are widely used in my country. The general structural formula of organophosphorus pesticides is X=PZ(R1)(R2), where X represents =O or =S, Z represents halogen, alkoxy or other substituents, etc., R1 and R2 represent methoxy (CH 3 o - ) or ethoxy (C 2 h 5 o - ). The molecular structure of organophosphorus pesticides is divided into two categories: one is P=O, such as dichlorvos, omethoate, etc.; the other is P=S, such as phorate, pirimiphos-methyl, etc. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 康怀志刘国坤曾勇明陈启振
Owner XIAMEN UNIV