A kind of preparation method of dna base pair cross-linked supramolecular hydrogel

Abstract

A method for preparing a DNA base-pair cross-linked supramolecular hydrogel of the present invention, respectively modifying adenine and thymine to obtain base monomers connected with double bonds, using water as a solvent, and mixing with hydrophilic Acrylamide, a non-reactive monomer, is thermally initiated by the initiator potassium persulfate, and a polymer with a three-dimensional network structure is formed by free radical copolymerization. The polymer relies on the hydrogen bond of complementary pairing of DNA base pairs to obtain DNA base pair interactions linked supramolecular hydrogels. The present invention introduces the base pairs contained in the organism into the polyacrylamide hydrogel, not just the terminal insertion, but the base pairs are embedded in the polymer chain segment, so that there are a large number of bases between the chains The base has a hydrogen bond effect, affects the mechanical properties and has a stronger and more sensitive response to salt and pH. And exclude the influence of organic solvents, have good biocompatibility, viscosity, pH and salt responsiveness, and can be used in fields such as gene delivery, gene therapy, drug controllable release and tissue engineering.

Description

technical field [0001] The invention belongs to the technical field of polymer hydrogel, and relates to a method for preparing DNA base-pair cross-linked supramolecular hydrogel. Background technique [0002] With the establishment of supramolecular science, supramolecular hydrogels have also begun to attract widespread attention. Compared with traditional chemically cross-linked hydrogels, the three-dimensional network structure of supramolecular hydrogels is induced by weak interactions. Weak interactions are sensitive to external stimuli and often have certain reversibility. Therefore, supramolecular hydrogels not only maintain the properties of traditional hydrogels such as high water content, biocompatibility, and environmental friendliness, but also often have stress Response, self-healing and other excellent properties. At present, the hydrogen bonding in supramolecular hydrogels is mostly intermolecular hydrogen bonding in non-biological organisms, while the hydroge...

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Problems solved by technology

[0003] At present, the reports about base pair hydrogels in the literature all introduce base pairs into one or both ends of the macromolecular chain segments in the hydrogel system to form hydrogen bonds between the chain segments, but this type of hydrogel The main cross-linking effect of the glue is the directional arrangement and stacking effect between the chain segments, which has the disadvantage of unstable structure

Moreover, most of these hydrogels are gelled in organic solvents and then removed with deionized water. However, there will be solvent residues after washing, and organic solvents are irritating and toxic to the human body and will cause adverse reactions. This limits the biological applications of such hydrogels

[0004] Polyacrylamide hydrogel has certain biocompatibility and can be applied to human soft tissue filling materials. It has been approved for cosmetic operations such as breast augmentation and hip augmentation, but its structure is unstable, and it cannot maintain its original shape after long-term water absorption and swelling. shape, and become a sol state, which limits its application range to a large extent

[0005] Chinese patent (CN 103694426 A) describes a base pair modification that is introduced into the end of the polymer, through the formation of complementary base pairs between the two bases, and the hydrogen bond formed between the base pairs has a salt and pH responsiveness, although the polymer can be used in seawater desalination and personal health care, etc., but the invention has the disadvantages of unstable structure and slow response to salt and pH due to low base content

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